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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_000169
  • pan locus tag?: SAUPAN001093000
  • symbol: JSNZ_000169
  • pan gene symbol?: fadB
  • synonym:
  • product: 3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA hydratase family protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_000169
  • symbol: JSNZ_000169
  • product: 3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA hydratase family protein
  • replicon: chromosome
  • strand: -
  • coordinates: 205219..207480
  • length: 2262
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGACAATTAATAAAGTAATCGTTCTTGGCGCAGGCACGATGGGCGCTCAACTGGCAGCA
    CTCTTTGTGAATGCTGGACTTAAAGTAAAACTATTAGATATTGTAGTGGACAAAAACGAT
    CCAAATCTCATTGCGAAAAAATCTTACGATAAAATTACAGATAAGAAACGGCCGCTACTA
    TTCGACTTAAATCTAGCGAGTCATTTAACATATGGTAATTTTGATGATGACTTGGTAAAT
    GATGATGCTGATTTATATATCGAAGCAGTCAAAGAAGATATTGAAATTAAGCATGCTGTT
    TGGCAACAAGTTCTACAACATGCTAAAGAAGATGCTTTATTTGCTACAAATACATCAGGT
    ATTCCAATTAATGCGATTGCTCAAGCATTTAACGAGAAGGATCAAGAACGATTCTTTGGT
    CTACATTTCTTTAACCCACCACGTATTATGAAATTAGTGGAGTTAATACCTACGTCACAC
    ACGAAGGAATCTATTATATTAGATGTAAAAAATTTCGCGCAAAATGTGTTAGGTAAAGGT
    GTCATTGTCGTCAATGATGTGCCTGGCTTTGTCGCAAATAGAGTCGGCACGCAAACAATG
    AATGATATTATGTATCGCGCCGAGCAACACAAGATAAGCATTGTAGATGTGGATGCTTTA
    ACTGGGCAAGCGATTGGTCGTCCTAAAACAGGTACATATGCGCTATCTGACCTAGTCGGT
    TTAGATATTGCAGTGTCTGTAATTAAAGGCATGCAACAAGTACCTGAAGAAACACCTTAT
    TTTCATGATGTCAAAATTGTAAATACGTTGTTTGAAAATGGCGCACTCGGACGTAAAACG
    AAACAAGGATTTTACAAAAAGGATAAAGAAACTAAAGCTCGGCTTGTTTACGATGTTGAA
    AAACAAGATTATGTACCTGTATCGCAACCACAATTACCAATTTTAAATGAATTTAATAAA
    GACTTAGTGCATAACCTTGATACCATATTCAATGCGCAAGACGAAGCGGGACTATTTTTA
    TGGGAGACATTACGTAATAATTTCTATTACTCTGCTATCAATGTACCTAAAGCTACCGAT
    GATTTCCGAGACATAGACCGTGCGCTTGTCTGGGGGTTCAACTGGAAACTTGGTCCATTC
    CAATTATGGGATGCAATGGGATATGAACGTGTTAAAAAACGTATAGAAGACGAACTTGGA
    GACTTACCACAATGGATTAGTGATTTAGATGGTGGCTTTTATAAACAAGATGAGACCATC
    GAATATGCAACACCTGTTTCTCACTTCGTAAAAGATGAACTTTGGGATAAAGGTGATGCC
    AAACTTTCCGTAACCCATGATAATCAACTGTTACTGAAATTACAAAGTAAAAATAATGTC
    ATTACCGATGAATTCAACGATGCGTTAGTTGATGCCATTGATTTACTGGAAAATGACCAT
    TACACAAGTATGGTCATTTATACAGATGGTAACAATTTCAGTGTGGGTGCTAACCTTTTC
    TTAATGAAAAAGGCGCATGAAGACGGTCTTGTAGATGATGTCGTTGCACAATCAATTGAT
    AAATTACATTATAGCTTTAATCGTTTGAAGTATAGTTTGAAACCAGTAGTCACAGCTGTT
    CAAGGTCGTGCCTTAGGCGGTGGCTGTGAGCTTGTACTTTACTCACCTATTGTTGTTTCT
    GCAAGTGAAACGTATATCGGTCTTGTTGAAGCAGGCGTTGGCTTATTACCAAGTGGCGGT
    GGCCTTGCAGAAATGGCTGATCGCATATTACGCACATCGCATAAGTTTGATGACAAACAA
    GCTTCCATGACAAAAGTATTGACGAATATCGCATTTGCGAAAGTCTCTACAAATGCCTTT
    GAGGCACGTCGTTATGGTTATTTACGTGATACAGATACGATTATTTTCAATACAGCACAA
    CGTGTCGAAGTTGCGCTCAAACGTGCGAAATATGAAGCAGAAACAAACTATATTCCGAAT
    CCTAGACATCAATATATCGCTTTAGGTGAAGACTTCAAAGCATTGATCCAAGGACAATTA
    GATGCGCAAAGACGGGGTCATTTTATTAGCGACCATGATTATCATATCGCCTTAAATATT
    GCCACAATTTTAACGGGTGGTGATTTACCAAGAAATACATTTATCAATCAACGTTACATT
    CAATCGTTGGAGAAAATTGGCTTTATTGACTTACTAAAATCTAAAAAATCATATGAAAGA
    ATTGCACATATGTTAAAAACTGGTAAGCCATTACGTAATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2160
    2220
    2262

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_000169
  • symbol: JSNZ_000169
  • description: 3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA hydratase family protein
  • length: 753
  • theoretical pI: 6.0383
  • theoretical MW: 84703.9
  • GRAVY: -0.247145

⊟Function[edit | edit source]

  • TIGRFAM:
    fatty acid oxidation complex, alpha subunit, mitochondrial (TIGR02441; EC 1.1.1.35,4.2.1.17; HMM-score: 173.3)
    Metabolism Fatty acid and phospholipid metabolism Degradation fatty oxidation complex, alpha subunit FadB (TIGR02437; EC 1.1.1.35,4.2.1.17,5.1.2.3,5.3.3.8; HMM-score: 167.6)
    Metabolism Fatty acid and phospholipid metabolism Degradation fatty oxidation complex, alpha subunit FadJ (TIGR02440; EC 1.1.1.35,4.2.1.17,5.1.2.3; HMM-score: 154.9)
    and 10 more
    3-hydroxyacyl-CoA dehydrogenase PaaC (TIGR02279; EC 1.1.1.-; HMM-score: 135.3)
    phenylacetate degradation probable enoyl-CoA hydratase PaaB (TIGR02280; EC 4.2.1.17; HMM-score: 39.4)
    cyclohexa-1,5-dienecarbonyl-CoA hydratase (TIGR03189; EC 4.2.1.100; HMM-score: 26.6)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone naphthoate synthase (TIGR01929; EC 4.1.3.36; HMM-score: 16.4)
    2-ketocyclohexanecarboxyl-CoA hydrolase (TIGR03210; EC 3.7.-.-; HMM-score: 16)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Chlorophyll and bacteriochlorphyll geranylgeranyl reductase family (TIGR02032; EC 1.3.1.-; HMM-score: 13.9)
    Metabolism Amino acid biosynthesis Glutamate family pyrroline-5-carboxylate reductase (TIGR00112; EC 1.5.1.2; HMM-score: 13.3)
    benzoyl-CoA-dihydrodiol lyase (TIGR03222; EC 4.1.2.44; HMM-score: 12.7)
    nucleotide sugar dehydrogenase (TIGR03026; HMM-score: 12.2)
    Metabolism Energy metabolism Amino acids and amines alanine dehydrogenase (TIGR00518; EC 1.4.1.1; HMM-score: 12.1)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    NADP_Rossmann (CL0063) 3HCDH_N; 3-hydroxyacyl-CoA dehydrogenase, NAD binding domain (PF02737; HMM-score: 163.1)
    and 6 more
    6PGD_C (CL0106) 3HCDH; 3-hydroxyacyl-CoA dehydrogenase, C-terminal domain (PF00725; HMM-score: 91.9)
    ClpP_crotonase (CL0127) ECH_1; Enoyl-CoA hydratase/isomerase (PF00378; HMM-score: 58)
    ECH_2; Enoyl-CoA hydratase/isomerase (PF16113; HMM-score: 33.7)
    NADP_Rossmann (CL0063) NAD_binding_2; NAD binding domain of 6-phosphogluconate dehydrogenase (PF03446; HMM-score: 14.7)
    Pyr_redox; Pyridine nucleotide-disulphide oxidoreductase (PF00070; HMM-score: 13.8)
    CheY (CL0304) NARF; Nucleotidyltransferase-Associated Rossmannoid Fold (PF20299; HMM-score: 11.2)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helix: 1
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9425
    • Cytoplasmic Membrane Score: 0.014
    • Cell wall & surface Score: 0.001
    • Extracellular Score: 0.0425
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.165436
    • TAT(Tat/SPI): 0.00868
    • LIPO(Sec/SPII): 0.025654
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MTINKVIVLGAGTMGAQLAALFVNAGLKVKLLDIVVDKNDPNLIAKKSYDKITDKKRPLLFDLNLASHLTYGNFDDDLVNDDADLYIEAVKEDIEIKHAVWQQVLQHAKEDALFATNTSGIPINAIAQAFNEKDQERFFGLHFFNPPRIMKLVELIPTSHTKESIILDVKNFAQNVLGKGVIVVNDVPGFVANRVGTQTMNDIMYRAEQHKISIVDVDALTGQAIGRPKTGTYALSDLVGLDIAVSVIKGMQQVPEETPYFHDVKIVNTLFENGALGRKTKQGFYKKDKETKARLVYDVEKQDYVPVSQPQLPILNEFNKDLVHNLDTIFNAQDEAGLFLWETLRNNFYYSAINVPKATDDFRDIDRALVWGFNWKLGPFQLWDAMGYERVKKRIEDELGDLPQWISDLDGGFYKQDETIEYATPVSHFVKDELWDKGDAKLSVTHDNQLLLKLQSKNNVITDEFNDALVDAIDLLENDHYTSMVIYTDGNNFSVGANLFLMKKAHEDGLVDDVVAQSIDKLHYSFNRLKYSLKPVVTAVQGRALGGGCELVLYSPIVVSASETYIGLVEAGVGLLPSGGGLAEMADRILRTSHKFDDKQASMTKVLTNIAFAKVSTNAFEARRYGYLRDTDTIIFNTAQRVEVALKRAKYEAETNYIPNPRHQYIALGEDFKALIQGQLDAQRRGHFISDHDYHIALNIATILTGGDLPRNTFINQRYIQSLEKIGFIDLLKSKKSYERIAHMLKTGKPLRN

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • data available for N315

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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