NCBI: 26-AUG-2013

⊟Summary[edit | edit source]

organism: Staphylococcus aureus N315

locus tag: SA2327 [new locus tag: SA_RS13340 ]

pan locus tag^?: SAUPAN006196000

symbol: SA2327

pan gene symbol^?: cidC

synonym:
product: pyruvate oxidase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

type: CDS
locus tag: SA2327 [new locus tag: SA_RS13340 ]
symbol: SA2327
product: pyruvate oxidase
replicon: chromosome
strand: -
coordinates: 2611919..2613658
length: 1740
essential: no DEG other strains

⊟Accession numbers[edit | edit source]

Gene ID: 1125254 NCBI
RefSeq: NP_375651 NCBI
BioCyc: see SA_RS13340
MicrobesOnline: 104677 MicrobesOnline

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

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ATGGCAAAAATAAAAGCAAATGAAGCATTAGTTAAAGCATTACAAGCATGGGATATAGAT
CACTTGTATGGTATTCCAGGAGACTCAATCGACGCAGTTGTCGATAGTTTACGTACAGTG
AGAGATCAATTTAAATTTTATCATGTACGTCATGAAGAAGTAGCAAGCTTAGCGGCTGCT
GGTTACACAAAATTAACTGGTAAAATCGGTGTGGCATTAAGTATCGGTGGCCCTGGTTTA
ATTCATTTATTAAATGGTATGTACGATGCCAAAATGGATAATGTACCGCAATTAATATTA
TCTGGACAAACGAATAGTACAGCACTTGGAACGAAAGCATTTCAAGAAACAAATTTACAA
AAATTATGTGAAGATGTAGCCGTTTATAATCACCAAATTGAAAAAGGTGACAATGTGTTT
GAAATCGTTAACGAAGCAATTCGTACGGCATATGAACAAAAAGGTGTCGCTGTTGTTATT
TGTCCTAACGACTTATTAACTGAAAAAATTAAAGATACAACGAATAAACCAGTAGATACA
TCAAGACCAACAGTTGTATCACCAAAATATAAAGACATCAAAAAAGCGGTTAAACTAATT
AATAAAAGTAAAAAGCCTGTCATGTTAATTGGTGTAGGTGCAAAACATGCGAAAGATGAG
CTACGTGAATTTATTGAAATGGCTAAAATTCCTGTCATTCATTCATTACCAGCTAAAACA
ATCTTGCCGGATGATCATCCATATAGTATCGGTAACTTAGGTAAAATTGGTACCAAAACA
TCTTATCAAACAATGCAGGAAGCGGATTTATTAATTATGGTTGGTACAAACTATCCATAT
GTGGATTACTTACCTAAGAAAAATATTAAAGCAATCCAAATCGACACAAATCCTAAAAAT
ATCGGACATCGTTTTAATATTAATGTAGGAATTGTTGGAGACAGTAAAATTGCATTGCAT
CAGTTAACTGAAAATATTAAACATGTTGCGGAAAGACCATTTTTAAACAAAACGTTAGAA
CGTAAAGCGGTTTGGGATAAATGGATGGAACAAGATAAAAATAATAATAGCAAACCATTA
CGTCCAGAACGTTTAATGGCATCAATTAACAAATTTATTAAAGATGATGCAGTAATTTCA
GCAGATGTTGGTACTGCAACAGTTTGGTCTACACGTTATTTAAATCTTGGTGTGAACAAC
AAGTTCATCATTTCAAGTTGGTTAGGTACAATGGGTTGCGGTCTTCCAGGAGCTATGGCA
TCAAAAATTGCATATCCAAATAGACAAGCGATTGCCATTGCTGGTGACGGTGCATTCCAA
ATGGTAATGCAAGACTTCGCTACAGCAGTACAATATGATTTACCTTTAACTGTATTTGTA
CTTAATAACAAACAGTTAGCATTTATTAAATATGAACAACAAGCAGCTGGTGAATTAGAA
TATGCAGTTGATTTTTCTGATATGGATCATGCAAAATTTGCTGAGGCAGCAGGTGGTAAA
GGTTATACAATTAAGAGTGCTAGCGAAGTAGATGCTATAGTCGAAGAGGCATTAGCACAA
GATGTACCAACGATTGTAGATGTATATGTTGATCCTAATGCTGCGCCATTACCAGGTAAA
ATTGTAAATGAAGAAGCGCTTGGTTATGGTAAATGGGCATTTAGATCTATTACTGAAGAT
AAATATTTAGATTTAGATCAAATTCCACCTATTTCAGTTGCAGCAAAACGTTTCTTATAA
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⊟Protein[edit | edit source]

⊟General[edit | edit source]

locus tag: SA2327 [new locus tag: SA_RS13340 ]
symbol: SA2327
description: pyruvate oxidase
length: 579
theoretical pI: 7.40736
theoretical MW: 63801
GRAVY: -0.173402

⊟Function[edit | edit source]

⊞⊞reaction:
EC 1.2.3.3? ExPASy
Pyruvate oxidase Pyruvate + phosphate + O₂ = acetyl phosphate + CO₂ + H₂O₂
⊞⊞TIGRFAM:
Metabolism Energy metabolism Aerobic pyruvate oxidase (TIGR02720; EC 1.2.3.3; HMM-score: 491.3)
and 14 more
Metabolism Amino acid biosynthesis Pyruvate family acetolactate synthase, large subunit, biosynthetic type (TIGR00118; EC 2.2.1.6; HMM-score: 367.2)
Metabolism Energy metabolism Fermentation acetolactate synthase, catabolic (TIGR02418; EC 2.2.1.6; HMM-score: 269.6)
Metabolism Central intermediary metabolism Other sulfoacetaldehyde acetyltransferase (TIGR03457; EC 2.3.3.15; HMM-score: 257.7)
glyoxylate carboligase (TIGR01504; EC 4.1.1.47; HMM-score: 187.8)
Cellular processes Cellular processes Detoxification oxalyl-CoA decarboxylase (TIGR03254; EC 4.1.1.8; HMM-score: 182.6)
Metabolism Energy metabolism Sugars 3,5/4-trihydroxycyclohexa-1,2-dione hydrolase (TIGR04377; EC 3.7.1.-; HMM-score: 174.8)
indolepyruvate/phenylpyruvate decarboxylase (TIGR03394; EC 4.1.1.43,4.1.1.74; HMM-score: 95.1)
Metabolism Central intermediary metabolism Other indolepyruvate decarboxylase (TIGR03393; EC 4.1.1.74; HMM-score: 93.5)
Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylic-acid synthase (TIGR00173; EC 2.2.1.9; HMM-score: 29)
phosphonopyruvate decarboxylase (TIGR03297; EC 4.1.1.82; HMM-score: 27.4)
sulfopyruvate decarboxylase, beta subunit (TIGR03846; EC 4.1.1.79; HMM-score: 19.9)
Metabolism Energy metabolism Chemoautotrophy CO dehydrogenase/acetyl-CoA synthase complex, epsilon subunit (TIGR00315; EC 1.2.99.2; HMM-score: 15)
Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other sulfopyruvate decarboxylase, alpha subunit (TIGR03845; EC 4.1.1.79; HMM-score: 14.6)
Metabolism Energy metabolism Methanogenesis sulfopyruvate decarboxylase, alpha subunit (TIGR03845; EC 4.1.1.79; HMM-score: 14.6)
⊞⊞
TheSEED :
- Pyruvate dehydrogenase (quinone) (EC 1.2.5.1)
- Pyruvate oxidase, CidC
Carbohydrates Central carbohydrate metabolism Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate Pyruvate oxidase [ubiquinone, cytochrome] (EC 1.2.2.2)
and 1 more
Regulation and Cell signaling Programmed Cell Death and Toxin-antitoxin Systems Murein hydrolase regulation and cell death Pyruvate oxidase, CidC
⊞⊞PFAM:
THDP-binding (CL0254) TPP_enzyme_C; Thiamine pyrophosphate enzyme, C-terminal TPP binding domain (PF02775; HMM-score: 147.5)
NADP_Rossmann (CL0063) TPP_enzyme_M; Thiamine pyrophosphate enzyme, central domain (PF00205; HMM-score: 128.7)
and 4 more
THDP-binding (CL0254) TPP_enzyme_N; Thiamine pyrophosphate enzyme, N-terminal TPP binding domain (PF02776; HMM-score: 99.9)
POR_N; Pyruvate flavodoxin/ferredoxin oxidoreductase, thiamine diP-bdg (PF01855; HMM-score: 16.1)
no clan defined PrpR_N; Propionate catabolism activator (PF06506; HMM-score: 13.5)
NADP_Rossmann (CL0063) CO_dh; CO dehydrogenase beta subunit/acetyl-CoA synthase epsilon subunit (PF02552; HMM-score: 13.3)

⊟Structure, modifications & cofactors[edit | edit source]

domains:
modifications:
cofactors:
effectors:

⊟Localization[edit | edit source]

⊞⊞
PSORTb: Cytoplasmic Membrane
- Cytoplasmic Score: 1.05
- Cytoplasmic Membrane Score: 8.78
- Cellwall Score: 0.08
- Extracellular Score: 0.09
- Internal Helices: 0
⊞⊞
DeepLocPro: Cytoplasmic Membrane
- Cytoplasmic Score: 0.4642
- Cytoplasmic Membrane Score: 0.5026
- Cell wall & surface Score: 0.001
- Extracellular Score: 0.0322
⊞⊞
LocateP: Intracellular
- Prediction by SwissProt Classification: Cytoplasmic
- Pathway Prediction: No pathway
- Intracellular possibility: 1
- Signal peptide possibility: -1
- N-terminally Anchored Score: -1
- Predicted Cleavage Site: No CleavageSite
⊞⊞
SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.004655
- TAT(Tat/SPI): 0.00018
- LIPO(Sec/SPII): 0.000379
predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

GI: 15928118 NCBI
RefSeq: NP_375651 NCBI
UniProt: A0A0H3JNN5 UniProt

⊟Protein sequence[edit | edit source]

MAKIKANEALVKALQAWDIDHLYGIPGDSIDAVVDSLRTVRDQFKFYHVRHEEVASLAAAGYTKLTGKIGVALSIGGPGLIHLLNGMYDAKMDNVPQLILSGQTNSTALGTKAFQETNLQKLCEDVAVYNHQIEKGDNVFEIVNEAIRTAYEQKGVAVVICPNDLLTEKIKDTTNKPVDTSRPTVVSPKYKDIKKAVKLINKSKKPVMLIGVGAKHAKDELREFIEMAKIPVIHSLPAKTILPDDHPYSIGNLGKIGTKTSYQTMQEADLLIMVGTNYPYVDYLPKKNIKAIQIDTNPKNIGHRFNINVGIVGDSKIALHQLTENIKHVAERPFLNKTLERKAVWDKWMEQDKNNNSKPLRPERLMASINKFIKDDAVISADVGTATVWSTRYLNLGVNNKFIISSWLGTMGCGLPGAMASKIAYPNRQAIAIAGDGAFQMVMQDFATAVQYDLPLTVFVLNNKQLAFIKYEQQAAGELEYAVDFSDMDHAKFAEAAGGKGYTIKSASEVDAIVEEALAQDVPTIVDVYVDPNAAPLPGKIVNEEALGYGKWAFRSITEDKYLDLDQIPPISVAAKRFL

⊟Experimental data[edit | edit source]

experimentally validated: data available for COL, NCTC8325
protein localization: data available for COL
quantitative data / protein copy number per cell: data available for COL

⊟⊟interaction partners:

SA1305	(hu)	DNA-binding protein II ^[1] (data from MRSA252)
SA1112	(infB)	translation initiation factor IF-2 ^[1] (data from MRSA252)
SA1244	(odhB)	dihydrolipoamide succinyltransferase ^[1] (data from MRSA252)
SA0943-1	(pdhA)	pyruvate dehydrogenase E1 component subunit alpha ^[1] (data from MRSA252)
SA0944	(pdhB)	pyruvate dehydrogenase E1 component subunit beta ^[1] (data from MRSA252)
SA0946	(pdhD)	dihydrolipoamide dehydrogenase ^[1] (data from MRSA252)
SA0218	(pflB)	formate acetyltransferase ^[1] (data from MRSA252)
SA0496	(rplA)	50S ribosomal protein L1 ^[1] (data from MRSA252)
SA2044	(rplB)	50S ribosomal protein L2 ^[1] (data from MRSA252)
SA2047	(rplC)	50S ribosomal protein L3 ^[1] (data from MRSA252)
SA2033	(rplF)	50S ribosomal protein L6 ^[1] (data from MRSA252)
SA2029	(rplO)	50S ribosomal protein L15 ^[1] (data from MRSA252)
SA2040	(rplP)	50S ribosomal protein L16 ^[1] (data from MRSA252)
SA1084	(rplS)	50S ribosomal protein L19 ^[1] (data from MRSA252)
SA1473	(rplU)	50S ribosomal protein L21 ^[1] (data from MRSA252)
SA2042	(rplV)	50S ribosomal protein L22 ^[1] (data from MRSA252)
SA2045	(rplW)	50S ribosomal protein L23 ^[1] (data from MRSA252)
SA1099	(rpsB)	30S ribosomal protein S2 ^[1] (data from MRSA252)
SA2041	(rpsC)	30S ribosomal protein S3 ^[1] (data from MRSA252)
SAS052	(rpsD)	30S ribosomal protein S4 ^[1] (data from MRSA252)
SA2031	(rpsE)	30S ribosomal protein S5 ^[1] (data from MRSA252)
SA2016	(rpsI)	30S ribosomal protein S9 ^[1] (data from MRSA252)
SA2038	(rpsQ)	30S ribosomal protein S17 ^[1] (data from MRSA252)
SA2043	(rpsS)	30S ribosomal protein S19 ^[1] (data from MRSA252)
SA0506	(tuf)	elongation factor Tu ^[1] (data from MRSA252)
SA1709		hypothetical protein ^[1] (data from MRSA252)

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

MicrobesOnline: SA2327 < SA2328 < SA2329

⊟Regulation[edit | edit source]

data available for COL, NCTC8325, Newman

⊟Transcription pattern[edit | edit source]

S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

Aureolib: no data available

⊟Protein stability[edit | edit source]

half-life: no data available

⊞Biological Material[edit | edit source]

⊞Other Information[edit | edit source]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

↑ ^{Jump up to: 1.00} ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 ^1.12 ^1.13 ^1.14 ^1.15 ^1.16 ^1.17 ^1.18 ^1.19 ^1.20 ^1.21 ^1.22 ^1.23 ^1.24 ^1.25 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
J Proteome Res: 2011, 10(3);1139-50
[PubMed:21166474] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

[pubmed21166474-1] {Jump up to: 1.00} ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 ^1.12 ^1.13 ^1.14 ^1.15 ^1.16 ^1.17 ^1.18 ^1.19 ^1.20 ^1.21 ^1.22 ^1.23 ^1.24 ^1.25 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
J Proteome Res: 2011, 10(3);1139-50
[PubMed:21166474] [WorldCat.org] [DOI] (I p)

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