From AureoWiki
Jump to navigation Jump to search

NCBI: 26-AUG-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA1702 [new locus tag: SA_RS09765 ]
  • pan locus tag?: SAUPAN004901000
  • symbol: SA1702
  • pan gene symbol?: vraT
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA1702 [new locus tag: SA_RS09765 ]
  • symbol: SA1702
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 1948401..1949102
  • length: 702
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGACACACAAATATATATCAACGCAAATGTTGATCATTTTTACTGCATTAATGATTATT
    GCCAATTTTTACTACATATTTTTTGAAAAAATTGGCTTTTTACTCGTTCTATTATTGGGA
    TGTGTATTAGTTTATGTAGGATATCTTTATTTTCATAAAATACGTGGCCTTTTAGCGTTT
    TGGATAGGCGCGCTATTAATTGCATTCACATTATTGTCTAATAAGTATACAATCATCATC
    TTGTTCGTCTTTTTATTACTACTTATTGTGCGTTATTTAATACATAAATTTAAACCAAAA
    AAAGTAGTTGCGACGGATGAGGTTATGACTTCACCATCTTTTATTAAACAAAAGTGGTTT
    GGTGAGCAACGTACACCAGTTTATGTATATAAGTGGGAAGATGTCCAAATTCAACATGGA
    ATTGGCGACCTACATATTGACTTAACAAAAGCTGCAAATATTAAGGAAAATAATACCATT
    GTTGTTAGACACATTTTAGGTAAAGTGCAGGTTATATTGCCGGTTAATTACAATATTAAT
    TTACATGTAGCTGCTTTTTATGGAAGTACTTACGTGAATGAAAAATCATATAAAGTTGAA
    AATAACAATATTCATATTGAAGAAATGATGAAACCGGATAACTATACAGTTAATATCTAC
    GTATCAACGTTTATCGGAGACGTAGAGGTGATTTATCGATGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    702

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA1702 [new locus tag: SA_RS09765 ]
  • symbol: SA1702
  • description: hypothetical protein
  • length: 233
  • theoretical pI: 9.49778
  • theoretical MW: 27204.1
  • GRAVY: 0.479399

⊟Function[edit | edit source]

  • TIGRFAM:
    flavivirus envelope glycoprotein E, stem/anchor domain (TIGR04240; HMM-score: 9.2)
  • TheSEED  :
    • Membrane protein LiaF(VraT), specific inhibitor of LiaRS(VraRS) signaling pathway
    CBSS-176280.1.peg.1561  Transporter associated with VraSR
  • ⊞PFAM:
    Pec_lyase-like (CL0268) LiaF-like_C; Cell wall-active antibiotics response LiaF, C-terminal (PF09922; HMM-score: 111.3)
    and 4 more
    no clan defined DUF7649; Domain of unknown function (DUF7649) (PF24661; HMM-score: 18.1)
    Pec_lyase-like (CL0268) DUF4097; Toastrack DUF4097 (PF13349; HMM-score: 14.5)
    no clan defined MerC; MerC mercury resistance protein (PF03203; HMM-score: 12.8)
    SdpI; SdpI/YfhL protein family (PF13630; HMM-score: 9.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 4
  • ⊞DeepLocPro: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.0002
    • Cytoplasmic Membrane Score: 0.9984
    • Cell wall & surface Score: 0
    • Extracellular Score: 0.0013
  • ⊞LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.0013
    • TAT(Tat/SPI): 0.00021
    • LIPO(Sec/SPII): 0.07494
  • predicted transmembrane helices (TMHMM): 4

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MTHKYISTQMLIIFTALMIIANFYYIFFEKIGFLLVLLLGCVLVYVGYLYFHKIRGLLAFWIGALLIAFTLLSNKYTIIILFVFLLLLIVRYLIHKFKPKKVVATDEVMTSPSFIKQKWFGEQRTPVYVYKWEDVQIQHGIGDLHIDLTKAANIKENNTIVVRHILGKVQVILPVNYNINLHVAAFYGSTYVNEKSYKVENNNIHIEEMMKPDNYTVNIYVSTFIGDVEVIYR

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Susan Boyle-Vavra, Shouhui Yin, Dae Sun Jo, Christopher P Montgomery, Robert S Daum
VraT/YvqF is required for methicillin resistance and activation of the VraSR regulon in Staphylococcus aureus.
Antimicrob Agents Chemother: 2013, 57(1);83-95
[PubMed:23070169] [WorldCat.org] [DOI] (I p)

Retrieved from "http://fungenwikiserver.biologie.uni-greifswald.de/aureowiki/index.php?title=SA1702&oldid=92837"