PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA1702 [new locus tag: SA_RS09765 ]
• pan locus tag^?: SAUPAN004901000
• symbol: SA1702
• pan gene symbol^?: vraT
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA1702 [new locus tag: SA_RS09765 ]
• symbol: SA1702
• product: hypothetical protein
• replicon: chromosome
• strand: -
• coordinates: 1948401..1949102
• length: 702
• essential: no DEG other strains

⊟Accession numbers

• Gene ID: 1124586 NCBI
• RefSeq: NP_374993 NCBI
• BioCyc: see SA_RS09765
• MicrobesOnline: 104019 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  
  ATGACACACAAATATATATCAACGCAAATGTTGATCATTTTTACTGCATTAATGATTATT
  GCCAATTTTTACTACATATTTTTTGAAAAAATTGGCTTTTTACTCGTTCTATTATTGGGA
  TGTGTATTAGTTTATGTAGGATATCTTTATTTTCATAAAATACGTGGCCTTTTAGCGTTT
  TGGATAGGCGCGCTATTAATTGCATTCACATTATTGTCTAATAAGTATACAATCATCATC
  TTGTTCGTCTTTTTATTACTACTTATTGTGCGTTATTTAATACATAAATTTAAACCAAAA
  AAAGTAGTTGCGACGGATGAGGTTATGACTTCACCATCTTTTATTAAACAAAAGTGGTTT
  GGTGAGCAACGTACACCAGTTTATGTATATAAGTGGGAAGATGTCCAAATTCAACATGGA
  ATTGGCGACCTACATATTGACTTAACAAAAGCTGCAAATATTAAGGAAAATAATACCATT
  GTTGTTAGACACATTTTAGGTAAAGTGCAGGTTATATTGCCGGTTAATTACAATATTAAT
  TTACATGTAGCTGCTTTTTATGGAAGTACTTACGTGAATGAAAAATCATATAAAGTTGAA
  AATAACAATATTCATATTGAAGAAATGATGAAACCGGATAACTATACAGTTAATATCTAC
  GTATCAACGTTTATCGGAGACGTAGAGGTGATTTATCGATGA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  702
  

⊟Protein

⊟General

• locus tag: SA1702 [new locus tag: SA_RS09765 ]
• symbol: SA1702
• description: hypothetical protein
• length: 233
• theoretical pI: 9.49778
• theoretical MW: 27204.1
• GRAVY: 0.479399

⊟Function

• TIGRFAM:
  flavivirus envelope glycoprotein E, stem/anchor domain (TIGR04240; HMM-score: 9.2)
• TheSEED  :
  • Membrane protein LiaF(VraT), specific inhibitor of LiaRS(VraRS) signaling pathway
  CBSS-176280.1.peg.1561  Transporter associated with VraSR
• PFAM:
  no clan defined DUF2154; Cell wall-active antibiotics response 4TMS YvqF (PF09922; HMM-score: 109.1)
  and 2 more
  MerC; MerC mercury resistance protein (PF03203; HMM-score: 8.9)
  SdpI; SdpI/YhfL protein family (PF13630; HMM-score: 6.6)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 4
• LocateP: Multi-transmembrane
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.0013
  • TAT(Tat/SPI): 0.00021
  • LIPO(Sec/SPII): 0.07494
• predicted transmembrane helices (TMHMM): 4

⊟Accession numbers

• GI: 15927460 NCBI
• RefSeq: NP_374993 NCBI
• UniProt: A0A0H3JMN7 UniProt

⊟Protein sequence

• MTHKYISTQMLIIFTALMIIANFYYIFFEKIGFLLVLLLGCVLVYVGYLYFHKIRGLLAFWIGALLIAFTLLSNKYTIIILFVFLLLLIVRYLIHKFKPKKVVATDEVMTSPSFIKQKWFGEQRTPVYVYKWEDVQIQHGIGDLHIDLTKAANIKENNTIVVRHILGKVQVILPVNYNINLHVAAFYGSTYVNEKSYKVENNNIHIEEMMKPDNYTVNIYVSTFIGDVEVIYR

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: vraR < vraS < SA1702 < SA1703

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications

Susan Boyle-Vavra, Shouhui Yin, Dae Sun Jo, Christopher P Montgomery, Robert S Daum
VraT/YvqF is required for methicillin resistance and activation of the VraSR regulon in Staphylococcus aureus.
Antimicrob Agents Chemother: 2013, 57(1);83-95
[PubMed:23070169] [WorldCat.org] [DOI] (I p)