PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

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⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA0463 [new locus tag: SA_RS02670 ]
• pan locus tag^?: SAUPAN002246000
• symbol: SA0463
• pan gene symbol^?: —
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA0463 [new locus tag: SA_RS02670 ]
• symbol: SA0463
• product: hypothetical protein
• replicon: chromosome
• strand: +
• coordinates: 536239..537432
• length: 1194
• essential: no DEG other strains

⊟Accession numbers

• Gene ID: 1123254 NCBI
• RefSeq: NP_373715 NCBI
• BioCyc: see SA_RS02670
• MicrobesOnline: 102741 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

• 1
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  1141
  
  ATGGCACATACCATTACGATTGTTGGCTTAGGAAACTATGGCATTGATGATTTGCCGCTA
  GGGATATATAAATTTTTAAAGACACAAGATAAAGTTTATGCAAGAACGTTAGATCATCCA
  GTTATAGAATCATTGCAAGATGAATTAACATTTCAGAGTTTTGACCATGTTTATGAAGCA
  CATGACCAATTTGAAGATGTCTATAACGATATTGTGGCGCAATTGGTTGAAGCTGCTAAT
  GAAAAAGATATTGTCTATGCGGTTCCGGGTCATCCTAGAGTTGCTGAGACAACTACAGTG
  AAATTACTGGCTTTAGCAAAGGACAATACTGATATAGATGTGAAAGTTTTAGGTGGTAAA
  AGCTTTATTGATGATGTGTTTGAAGCAGTTAATGTAGATCCAAATGATGGCTTCACACTG
  TTAGATGCGACATCATTACAAGAAGTAATACTTAATGTTAGAACGCATACATTGATTACG
  CAAGTTTATAGTGCAATGGTTGCTGCTAATTTGAAAATCACTTTAATGGAACGATATCCT
  GATGATTACCCTGTTCAAATTGTCACTGGTGCACGAAGCGATGGTGCGGATAACGTTGTG
  ACATGCCCATTATATGAATTGGATCATGATGAAAATGCATTCAATAATTTGACGAGTGTA
  TTCGTACCAAAAATCATAACATCGACATATTTGTATCATGACTTTGATTTTGCAACGGAA
  GTGATTGATACTTTAGTTGATGAAGATAAAGGTTGTCCATGGGATAAAGTGCAAACGCAT
  GAAACGCTTAAGCGTTATTTACTTGAAGAAACATTTGAATTGTTCGAAGCTATTGACAAT
  GAAGATGATTGGCATATGATTGAAGAACTAGGAGATATTTTATTACAAGTGTTATTGCAT
  ACTAGTATTGGTAAAAAAGAAGGGTATATCGACATTAAAGAAGTGATTACAAGTCTTAAT
  GCTAAAATGATTCGTAGACACCCACACATATTTGGTGATGCCAATGCTGAAACTATCGAT
  GACTTAAAAGAAATTTGGTCTAAGGCGAAAGATGCTGAAGGTAAACAGCCAAGAGTTAAA
  TTTGAAAAAGTATTTGCAGAGCATTTTTTAAATTTATATGAGAAGACGAAGGATAAGTCA
  TTTGATGAGGCCTCGTTAAAGCAGTGGCTAGAAAAAGGGGAGAGTAATACATGA
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⊟Protein

⊟General

• locus tag: SA0463 [new locus tag: SA_RS02670 ]
• symbol: SA0463
• description: hypothetical protein
• length: 397
• theoretical pI: 4.34085
• theoretical MW: 45139.4
• GRAVY: -0.288413

⊟Function

• ⊞TIGRFAM:
  Unknown function General MazG family protein (TIGR00444; HMM-score: 132.4)
  and 2 more
  Genetic information processing Protein fate Protein modification and repair diphthine synthase (TIGR00522; EC 2.1.1.98; HMM-score: 31)
  Unknown function Enzymes of unknown specificity encapsulated protein (TIGR04536; HMM-score: 12.9)
• ⊞TheSEED  :
  • Nucleoside triphosphate pyrophosphohydrolase MazG (EC 3.6.1.8)
  Nucleosides and Nucleotides Detoxification Nucleoside triphosphate pyrophosphohydrolase MazG  Nucleoside triphosphate pyrophosphohydrolase MazG (EC 3.6.1.8)
  and 1 more
  Nucleosides and Nucleotides Detoxification Nucleoside triphosphate pyrophosphohydrolase MazG  possible tetrapyrrole methyltransferase domain
• ⊞PFAM:
  MazG (CL0231) MazG; MazG nucleotide pyrophosphohydrolase domain (PF03819; HMM-score: 101)
  and 3 more
  no clan defined TP_methylase; Tetrapyrrole (Corrin/Porphyrin) Methylases (PF00590; HMM-score: 64.3)
  MazG (CL0231) PRA-PH; Phosphoribosyl-ATP pyrophosphohydrolase (PF01503; HMM-score: 14.9)
  MazG-like; MazG-like family (PF12643; HMM-score: 13.1)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• ⊞PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• ⊞DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.9982
  • Cytoplasmic Membrane Score: 0.0006
  • Cell wall & surface Score: 0.0001
  • Extracellular Score: 0.0012
• ⊞LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• ⊞SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.002807
  • TAT(Tat/SPI): 0.000101
  • LIPO(Sec/SPII): 0.000241
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 15926182 NCBI
• RefSeq: NP_373715 NCBI
• UniProt: A0A0H3JKX8 UniProt

⊟Protein sequence

• MAHTITIVGLGNYGIDDLPLGIYKFLKTQDKVYARTLDHPVIESLQDELTFQSFDHVYEAHDQFEDVYNDIVAQLVEAANEKDIVYAVPGHPRVAETTTVKLLALAKDNTDIDVKVLGGKSFIDDVFEAVNVDPNDGFTLLDATSLQEVILNVRTHTLITQVYSAMVAANLKITLMERYPDDYPVQIVTGARSDGADNVVTCPLYELDHDENAFNNLTSVFVPKIITSTYLYHDFDFATEVIDTLVDEDKGCPWDKVQTHETLKRYLLEETFELFEAIDNEDDWHMIEELGDILLQVLLHTSIGKKEGYIDIKEVITSLNAKMIRRHPHIFGDANAETIDDLKEIWSKAKDAEGKQPRVKFEKVFAEHFLNLYEKTKDKSFDEASLKQWLEKGESNT

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: rplY > pth > mfd > SA0462 > SA0463 > SA0464 > SA0465 > SA0466

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications