PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_0483 [new locus tag: SAUSA300_RS02595 ]
• pan locus tag^?: SAUPAN002246000
• symbol: SAUSA300_0483
• pan gene symbol^?: —
• synonym:
• product: tetrapyrrole methylase family protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_0483 [new locus tag: SAUSA300_RS02595 ]
• symbol: SAUSA300_0483
• product: tetrapyrrole methylase family protein
• replicon: chromosome
• strand: +
• coordinates: 542819..544012
• length: 1194
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3914009 NCBI
• RefSeq: YP_493192 NCBI
• BioCyc: see SAUSA300_RS02595
• MicrobesOnline: 1291998 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGGCACATACCATTACGATTGTTGGCTTAGGAAACTATGGCATTGATGATTTGCCGCTA
  GGGATATATAAATTTTTAAAGACACAAGATAAAGTTTATGCAAGAACGTTAGATCATCCA
  GTTATAGAATCATTGCAAGATGAATTAACATTTCAGAGTTTTGACCATGTTTATGAAGCA
  CATAACCAATTTGAAGATGTCTATATTGATATTGTGGCGCAATTGGTTGAAGCTGCTAAT
  GAAAAAGATATTGTCTATGCGGTTCCGGGTCATCCTAGAGTTGCTGAGACAACTACAGTG
  AAATTACTGGCTTTAGCAAAGGACAATACTGATATAGATGTGAAAGTTTTAGGTGGGAAA
  AGCTTTATTGATGATGTGTTTGAAGCAGTTAATGTAGATCCAAATGATGGCTTCACACTG
  TTAGATGCGACATCATTACAAGAAGTAACACTTAATGTTAGAACGCATACATTGATTACG
  CAAGTTTATAGTGCAATGGTTGCTGCTAATTTGAAAATCACTTTAATGGAACGATATCCT
  GATGATTACCCTGTTCAAATTGTCACTGGTGCACGAAGCGATGGTGCGGATAACGTTGTG
  ACATGCCCATTATATGAATTGGATCATGATGAAAATGCATTCAATAATTTGACGAGTGTA
  TTCGTACCAAAAATCATAACATCGACATATTTGTATCATGACTTTGATTTTGCAACGGAA
  GTGATTGATACTTTAGTTGATGAAGATAAAGGTTGTCCATGGGATAAAGTGCAAACGCAT
  GAAACGCTAAAGCGTTATTTACTTGAAGAAACATTTGAATTGTTCGAAGCTATTGACAAT
  GAAGATGATTGGCATATGATTGAAGAACTAGGAGATATTTTATTACAAGTGTTATTGCAT
  ACTAGTATTGGTAAAAAAGAAGGGTATATCGACATTAAAGAAGTGATTACAAGTCTTAAT
  GCTAAAATGATTCGTAGACACCCACACATATTTGGTGATGCCAATGCTGAAACTATCGAT
  GACTTAAAAGAAATTTGGTCTAAGGCGAAAGATGCTGAAGGTAAACAGCCAAGAGTTATA
  TTTGAAAAAGTATTTGCAGAGCATTTTTTAAATTTATATGAGAAGACGAAGGATAAGTCA
  TTTGATGAGGCCGCGTTAAAGCAGTGGCTAGAAAAAGGGGAGAGTAATACATGA
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⊟Protein

⊟General

• locus tag: SAUSA300_0483 [new locus tag: SAUSA300_RS02595 ]
• symbol: SAUSA300_0483
• description: tetrapyrrole methylase family protein
• length: 397
• theoretical pI: 4.33351
• theoretical MW: 45094.4
• GRAVY: -0.253652

⊟Function

• TIGRFAM:
  Unknown function General MazG family protein (TIGR00444; HMM-score: 131.9)
  and 1 more
  Genetic information processing Protein fate Protein modification and repair diphthine synthase (TIGR00522; EC 2.1.1.98; HMM-score: 30.3)
• TheSEED  :
  • Nucleoside triphosphate pyrophosphohydrolase MazG (EC 3.6.1.8)
  • possible tetrapyrrole methyltransferase domain
  Nucleosides and Nucleotides Detoxification Nucleoside triphosphate pyrophosphohydrolase MazG  Nucleoside triphosphate pyrophosphohydrolase MazG (EC 3.6.1.8)
  and 1 more
  Nucleosides and Nucleotides Detoxification Nucleoside triphosphate pyrophosphohydrolase MazG  possible tetrapyrrole methyltransferase domain
• PFAM:
  MazG (CL0231) MazG; MazG nucleotide pyrophosphohydrolase domain (PF03819; HMM-score: 93.2)
  and 2 more
  no clan defined TP_methylase; Tetrapyrrole (Corrin/Porphyrin) Methylases (PF00590; HMM-score: 66.8)
  MazG (CL0231) MazG-like; MazG-like family (PF12643; HMM-score: 12.9)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.002823
  • TAT(Tat/SPI): 0.000109
  • LIPO(Sec/SPII): 0.000248
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87160529 NCBI
• RefSeq: YP_493192 NCBI
• UniProt: A0A0H2XGV2 UniProt

⊟Protein sequence

• MAHTITIVGLGNYGIDDLPLGIYKFLKTQDKVYARTLDHPVIESLQDELTFQSFDHVYEAHNQFEDVYIDIVAQLVEAANEKDIVYAVPGHPRVAETTTVKLLALAKDNTDIDVKVLGGKSFIDDVFEAVNVDPNDGFTLLDATSLQEVTLNVRTHTLITQVYSAMVAANLKITLMERYPDDYPVQIVTGARSDGADNVVTCPLYELDHDENAFNNLTSVFVPKIITSTYLYHDFDFATEVIDTLVDEDKGCPWDKVQTHETLKRYLLEETFELFEAIDNEDDWHMIEELGDILLQVLLHTSIGKKEGYIDIKEVITSLNAKMIRRHPHIFGDANAETIDDLKEIWSKAKDAEGKQPRVIFEKVFAEHFLNLYEKTKDKSFDEAALKQWLEKGESNT

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SAUSA300_0479 > pth > mfd > SAUSA300_0482 > SAUSA300_0483 > SAUSA300_0484 > SAUSA300_0485

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications