PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_0482 [new locus tag: SAUSA300_RS02590 ]
• pan locus tag^?: SAUPAN002245000
• symbol: SAUSA300_0482
• pan gene symbol^?: —
• synonym:
• product: polysaccharide biosynthesis protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_0482 [new locus tag: SAUSA300_RS02590 ]
• symbol: SAUSA300_0482
• product: polysaccharide biosynthesis protein
• replicon: chromosome
• strand: +
• coordinates: 541293..542819
• length: 1527
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3914914 NCBI
• RefSeq: YP_493191 NCBI
• BioCyc: see SAUSA300_RS02590
• MicrobesOnline: 1291997 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

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  ATGAAGCATAAAGAAGCATTTAATGGCGTTGTCGTGTTAACTGCTGCATTAATTGTCATT
  AAAATTCTGAGTGCTGTATATCGAATTCCATATCAAAATATATTAGGCGATACAGGTTTG
  TATGCATATCAACAAGTGTATCCAATTGTAGCATTAGGAATGATATTATCGATGAATGCC
  ATTCCTAGTGCAATTACACAAAATATAGGGAAGTATCATAGTGACGAAGCATATGCAAAA
  GCAGTCGCTTATATACAATTAGTTGGTATATTATTATTTATTGCTATTTTTGTGTTTGCG
  AACAATATTGCACATATGATGGGTGATGGCCATTTAACACCAATGATTCAAGCTGCAAGT
  TTAAGCTTTATATTTATAGGTATGCTTGGCGTGTTAAGAGGTTATTATCAATCTGCAAAT
  AATATGACAGTTCCGGCTATTTCCCAGGTTATAGAACAAGTTATACGAGTAGGTATTATC
  ATTGTTACTATTGTTATTTTTGTAGACAGAGGTTGGACGATATATGAAGCGGGAACAATT
  GCTATTTTAGCATCAACGATAGGTTTTTTAGGTTCTTCAATTTATTTAGTAGCGCACCGA
  CCTTTTAAGTTTAAAATGGTAAATAACACTGCAAAGATCGTTTGGAAACAGTTCGCACTT
  TCGGTTTTGATTTTCGCTATCAGTCAATTAATCGTAATTTTATGGCAAGTGATTGATAGT
  GTTACTATTATTAAGTCACTTCAAGCGATACGCGTGCCATTCGATGTTGCCATAACTGAA
  AAAGGAGTCTATGACCGTGGTGCATCATTTATTCAGATGGGATTGATTGTAACTACAACA
  TTTAGTTTTGCGCTCATTCCTCTGTTAAGTGACGCAATTAAAATGAATAATCAGGTACTT
  ATGAATCGTTATGCAAATGCGTCATTAAAGATTACGATTTTAATAAGTACAGCAGCAGGA
  ATAGGATTAATTAATTTATTGCCTTTAATGAACGGTGTGTTTTTTAAGACGAATGATTTA
  ACCTTAACGTTAAGTGTTTATATGATTACGGTCATTTGTGTATCGTTAATTATGATGGAT
  ATGGCATTATTACAAGCGCAACATGCTGTGAGACCTATTTTTGTTGGTATGACGGCAGGA
  TTGGTTATTAAATTTATACTTAATATCATTTTGATTCGTTTAAGTGGCATTATTGGTGCG
  AGCATTAGTACTGTTGTATCATTAATTATATTCGGTACGATTATCCATATTGCTGTCACG
  AGAAAATACCACTTATATGCGATGAGACGATTTTTTATCAATGTTGTTTTAGGTATGGTA
  TTTATGTCGATTGTTGTTCAATGCGTGTTAAACATAGTGACAACACACGGTAGAATCACT
  GGACTCATTGAATTATTATGTGCAGCAGTATTAGGTATCATTGCATTGTTTTTCTATATT
  TTTAGATTTAATGTTTTGACATATAAAGAGTTAACTTATTTACCATTTGGTTCAAAGTTG
  TATCAAATTAAGAAAGGAAGACGTTGA
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⊟Protein

⊟General

• locus tag: SAUSA300_0482 [new locus tag: SAUSA300_RS02590 ]
• symbol: SAUSA300_0482
• description: polysaccharide biosynthesis protein
• length: 508
• theoretical pI: 10.2142
• theoretical MW: 56089.3
• GRAVY: 0.951181

⊟Function

• TIGRFAM:
  Cellular processes Cellular processes Sporulation and germination stage V sporulation protein B (TIGR02900; HMM-score: 109.4)
• TheSEED  :
  • Low temperature requirement B protein
• PFAM:
  MviN_MATE (CL0222) Polysacc_synt; Polysaccharide biosynthesis protein (PF01943; HMM-score: 94.9)
  and 3 more
  Polysacc_synt_C; Polysaccharide biosynthesis C-terminal domain (PF14667; HMM-score: 67.3)
  Polysacc_synt_3; Polysaccharide biosynthesis protein (PF13440; HMM-score: 21.3)
  MVIN; MviN-like protein (PF03023; HMM-score: 10.1)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 14
• LocateP: Multi-transmembrane
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.097766
  • TAT(Tat/SPI): 0.001022
  • LIPO(Sec/SPII): 0.02855
• predicted transmembrane helices (TMHMM): 14

⊟Accession numbers

• GI: 87161203 NCBI
• RefSeq: YP_493191 NCBI
• UniProt: A0A0H2XI36 UniProt

⊟Protein sequence

• MKHKEAFNGVVVLTAALIVIKILSAVYRIPYQNILGDTGLYAYQQVYPIVALGMILSMNAIPSAITQNIGKYHSDEAYAKAVAYIQLVGILLFIAIFVFANNIAHMMGDGHLTPMIQAASLSFIFIGMLGVLRGYYQSANNMTVPAISQVIEQVIRVGIIIVTIVIFVDRGWTIYEAGTIAILASTIGFLGSSIYLVAHRPFKFKMVNNTAKIVWKQFALSVLIFAISQLIVILWQVIDSVTIIKSLQAIRVPFDVAITEKGVYDRGASFIQMGLIVTTTFSFALIPLLSDAIKMNNQVLMNRYANASLKITILISTAAGIGLINLLPLMNGVFFKTNDLTLTLSVYMITVICVSLIMMDMALLQAQHAVRPIFVGMTAGLVIKFILNIILIRLSGIIGASISTVVSLIIFGTIIHIAVTRKYHLYAMRRFFINVVLGMVFMSIVVQCVLNIVTTHGRITGLIELLCAAVLGIIALFFYIFRFNVLTYKELTYLPFGSKLYQIKKGRR

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SAUSA300_0479 > pth > mfd > SAUSA300_0482 > SAUSA300_0483 > SAUSA300_0484 > SAUSA300_0485

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications