PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

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⊟Summary

• organism: Staphylococcus aureus N315
locus tag: SA1700 [new locus tag: SA_RS09755 ]
pan locus tag^?: SAUPAN004899000
symbol: vraR
pan gene symbol^?: vraR
• synonym:
• product: two-component response regulator

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA1700 [new locus tag: SA_RS09755 ]
• symbol: vraR
• product: two-component response regulator
• replicon: chromosome
• strand: -
• coordinates: 1946742..1947371
• length: 630
• essential: no DEG other strains

⊟Accession numbers

• Gene ID: 1124584 NCBI
• RefSeq: NP_374991 NCBI
• BioCyc: see SA_RS09755
• MicrobesOnline: 104017 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  601
  
  ATGACGATTAAAGTATTGTTTGTGGATGATCATGAAATGGTACGTATAGGAATTTCAAGT
  TATCTATCAACGCAAAGTGATATTGAAGTAGTTGGTGAAGGCGCTTCTGGTAAAGAAGCA
  ATTGCCAAAGCCCATGAGTTGAAGCCAGATTTAATTTTAATGGATTTACTTATGGAAGAC
  ATGGATGGTGTAGAAGCGACGACTCAGATTAAAAAAGATTTACCGCAAATTAAAGTATTA
  ATGTTAACTAGTTTTATTGAAGATAAAGAGGTATATCGTGCATTAGATGCAGGTGTCGAT
  AGTTACATTTTAAAAACAACAAGTGCAAAAGATATCGCCGATGCAGTTCGTAAAACTTCT
  AGAGGAGAATCTGTTTTTGAACCGGAAGTTTTAGTGAAAATGCGTAACCGTATGAAAAAG
  CGCGCAGAGTTATATGAAATGCTTACAGAACGAGAAATGGAAATATTATTATTGATTGCG
  AAAGGTTACTCAAATCAAGAAATTGCTAGTGCATCGCATATTACTATTAAAACGGTTAAG
  ACACATGTGAGTAACATTTTAAGTAAGTTAGAAGTGCAAGATAGAACACAAGCTGTCATC
  TATGCATTCCAACATAATTTAATTCAATAG
  60
  120
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  630
  

⊟Protein

⊟General

• locus tag: SA1700 [new locus tag: SA_RS09755 ]
• symbol: VraR
• description: two-component response regulator
• length: 209
• theoretical pI: 5.3954
• theoretical MW: 23559.2
• GRAVY: -0.121531

⊟Function

• ⊞TIGRFAM:
  Cellular processes Cellular processes Sporulation and germination sporulation transcription factor Spo0A (TIGR02875; HMM-score: 72.4)
  and 15 more
  Signal transduction Regulatory functions DNA interactions phosphate regulon transcriptional regulatory protein PhoB (TIGR02154; HMM-score: 56.1)
  Signal transduction Signal transduction Two-component systems phosphate regulon transcriptional regulatory protein PhoB (TIGR02154; HMM-score: 56.1)
  transcriptional regulator EpsA (TIGR03020; HMM-score: 55.8)
  Signal transduction Regulatory functions DNA interactions heavy metal response regulator (TIGR01387; HMM-score: 36.5)
  Metabolism Central intermediary metabolism Nitrogen metabolism nitrogen regulation protein NR(I) (TIGR01818; HMM-score: 36.4)
  Signal transduction Regulatory functions DNA interactions nitrogen regulation protein NR(I) (TIGR01818; HMM-score: 36.4)
  Signal transduction Signal transduction Two-component systems nitrogen regulation protein NR(I) (TIGR01818; HMM-score: 36.4)
  LuxR family transcriptional regulatory, chaperone HchA-associated (TIGR03541; HMM-score: 34)
  RNA polymerase sigma-70 factor, Bacteroides expansion family 1 (TIGR02985; HMM-score: 26.6)
  Signal transduction Signal transduction Two-component systems TMAO reductase sytem sensor TorS (TIGR02956; EC 2.7.13.3; HMM-score: 25.5)
  RNA polymerase sigma factor, sigma-70 family (TIGR02937; HMM-score: 20.9)
  Cellular processes Cellular processes Sporulation and germination RNA polymerase sigma-H factor (TIGR02859; HMM-score: 17.1)
  Genetic information processing Transcription Transcription factors RNA polymerase sigma-H factor (TIGR02859; HMM-score: 17.1)
  proteobacterial dedicated sortase system response regulator (TIGR03787; HMM-score: 15.9)
  RNA polymerase sigma-70 factor, sigma-E family (TIGR02983; HMM-score: 13.4)
• TheSEED  :
  • Cell envelope stress response system LiaFSR, response regulator LiaR(VraR)
  CBSS-176280.1.peg.1561  Two component transcriptional regulator VraR
• ⊞PFAM:
  CheY (CL0304) Response_reg; Response regulator receiver domain (PF00072; HMM-score: 93.5)
  and 14 more
  HTH (CL0123) GerE; Bacterial regulatory proteins, luxR family (PF00196; HMM-score: 71.1)
  Sigma70_r4_2; Sigma-70, region 4 (PF08281; HMM-score: 33.6)
  HTH_23; Homeodomain-like domain (PF13384; HMM-score: 16.6)
  HTH_40; Helix-turn-helix domain (PF14493; HMM-score: 16.6)
  UPF0122; Putative helix-turn-helix protein, YlxM / p13 like (PF04297; HMM-score: 15.6)
  Sigma70_r4; Sigma-70, region 4 (PF04545; HMM-score: 15.5)
  HTH_24; Winged helix-turn-helix DNA-binding (PF13412; HMM-score: 15.3)
  no clan defined DUF3194; Protein of unknown function (DUF3194) (PF11419; HMM-score: 15)
  HTH (CL0123) HTH_28; Helix-turn-helix domain (PF13518; HMM-score: 13.7)
  wHTH-PRTase_assc; wHTH-PRTase associated (PF24409; HMM-score: 13.6)
  PDDEXK (CL0236) PDDEXK_10; PD-(D/E)XK nuclease superfamily (PF07788; HMM-score: 13.5)
  no clan defined B12-binding; B12 binding domain (PF02310; HMM-score: 12.9)
  HTH (CL0123) HTH_38; Helix-turn-helix domain (PF13936; HMM-score: 12.9)
  GT-A (CL0110) Rhamno_transf; Putative rhamnosyl transferase (PF11316; HMM-score: 12.8)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• ⊞PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• ⊞DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.9872
  • Cytoplasmic Membrane Score: 0.0016
  • Cell wall & surface Score: 0.0001
  • Extracellular Score: 0.0111
• ⊞LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• ⊞SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.006685
  • TAT(Tat/SPI): 0.000295
  • LIPO(Sec/SPII): 0.000629
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 15927458 NCBI
• RefSeq: NP_374991 NCBI
• UniProt: Q7A4R9 UniProt

⊟Protein sequence

• MTIKVLFVDDHEMVRIGISSYLSTQSDIEVVGEGASGKEAIAKAHELKPDLILMDLLMEDMDGVEATTQIKKDLPQIKVLMLTSFIEDKEVYRALDAGVDSYILKTTSAKDIADAVRKTSRGESVFEPEVLVKMRNRMKKRAELYEMLTEREMEILLLIAKGYSNQEIASASHITIKTVKTHVSNILSKLEVQDRTQAVIYAFQHNLIQ

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: vraR < vraS < SA1702 < SA1703

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications

Makoto Kuroda, Hiroko Kuroda, Taku Oshima, Fumihiko Takeuchi, Hirotada Mori, Keiichi Hiramatsu
Two-component system VraSR positively modulates the regulation of cell-wall biosynthesis pathway in Staphylococcus aureus.
Mol Microbiol: 2003, 49(3);807-21
[PubMed:12864861] [WorldCat.org] [DOI] (P p)Mrittika Sengupta, Vaibhav Jain, Brian J Wilkinson, Radheshyam K Jayaswal
Chromatin immunoprecipitation identifies genes under direct VraSR regulation in Staphylococcus aureus.
Can J Microbiol: 2012, 58(6);703-8
[PubMed:22571705] [WorldCat.org] [DOI] (I p)