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NCBI: 06-JUL-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_1115 [new locus tag: NWMN_RS06300 ]
  • pan locus tag?: SAUPAN003484000
  • symbol: pryF
  • pan gene symbol?: pyrF
  • synonym:
  • product: orotidine 5'-phosphate decarboxylase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_1115 [new locus tag: NWMN_RS06300 ]
  • symbol: pryF
  • product: orotidine 5'-phosphate decarboxylase
  • replicon: chromosome
  • strand: +
  • coordinates: 1222190..1222882
  • length: 693
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGAAAGATTTACCAATTATTGCATTAGATTTTGAATCAAAAGAAAAAGTAAATCAATTT
    TTAGATTTATTTGATGAATCATTATTCGTAAAAGTAGGTATGGAACTTTTTTATCAAGAA
    GGTCCTCAATTAATTAATGAGATAAAAGAAAGAGGCCATGATGTATTTTTAGATTTAAAA
    CTGCATGATATTCCTAATACAGTTGGTAAGGCGATGGAAGGACTAGCTAAATTGAATGTT
    GATCTGGTAAATGTTCATGCTGCTGGTGGCGTAAAAATGATGTCTGAGGCCATTAAAGGA
    TTAAGAAAACATAATCAAGATACAAAAATTATTGCAGTAACACAGCTTACGTCAACAACA
    GAAGACATGTTACGACACGAACAAAATATACAAACATCGATTGAAGAGGCCGTTTTAAAT
    TATGCCAAGTTAGCAAATGCAGCTGGTTTAGATGGCGTTGTTTGTTCACCTCTTGAAAGT
    CGTATGTTGACTGAAAAGTTAGGTACATCATTTTTAAAAGTAACACCAGGTATTAGACCT
    AAAGGTGCATCTCAAAATGACCAACACCGTATTACGACACCGGAAGAAGCAAGACAGCTT
    GGTTCGACGCATATTGTAGTCGGTAGACCGATTACACAAAGTGACAATCCAGTCGAAAGT
    TATCATAAAATTAAAGAAAGTTGGTTAGTATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    693

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_1115 [new locus tag: NWMN_RS06300 ]
  • symbol: PryF
  • description: orotidine 5'-phosphate decarboxylase
  • length: 230
  • theoretical pI: 6.09702
  • theoretical MW: 25596.2
  • GRAVY: -0.293478

⊟Function[edit | edit source]

  • ⊞reaction:
    EC 4.1.1.23?  ExPASy
    Orotidine-5'-phosphate decarboxylase Orotidine 5'-phosphate = UMP + CO2
  • ⊞TIGRFAM:
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Pyrimidine ribonucleotide biosynthesis orotidine 5'-phosphate decarboxylase (TIGR01740; EC 4.1.1.23; HMM-score: 206.1)
    and 2 more
    3-hexulose-6-phosphate synthase (TIGR03128; EC 4.1.2.43; HMM-score: 22)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pyridoxine pyridoxal 5'-phosphate synthase, synthase subunit Pdx1 (TIGR00343; HMM-score: 12.9)
  • TheSEED  :
    • Orotidine 5'-phosphate decarboxylase (EC 4.1.1.23)
    Nucleosides and Nucleotides Pyrimidines De Novo Pyrimidine Synthesis  Orotidine 5'-phosphate decarboxylase (EC 4.1.1.23)
  • ⊞PFAM:
    TIM_barrel (CL0036) OMPdecase; Orotidine 5'-phosphate decarboxylase / HUMPS family (PF00215; HMM-score: 221.4)
    and 2 more
    no clan defined DUF3842; Domain of unknown function (DUF3842) (PF12953; HMM-score: 13.9)
    DUF2120; Uncharacterized protein conserved in archaea (DUF2120) (PF09893; HMM-score: 13.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • ⊞DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9996
    • Cytoplasmic Membrane Score: 0.0002
    • Cell wall & surface Score: 0
    • Extracellular Score: 0.0002
  • ⊞LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.000682
    • TAT(Tat/SPI): 0.000081
    • LIPO(Sec/SPII): 0.000126
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKDLPIIALDFESKEKVNQFLDLFDESLFVKVGMELFYQEGPQLINEIKERGHDVFLDLKLHDIPNTVGKAMEGLAKLNVDLVNVHAAGGVKMMSEAIKGLRKHNQDTKIIAVTQLTSTTEDMLRHEQNIQTSIEEAVLNYAKLANAAGLDGVVCSPLESRMLTEKLGTSFLKVTPGIRPKGASQNDQHRITTPEEARQLGSTHIVVGRPITQSDNPVESYHKIKESWLV

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • ⊞regulator: pyrR leader (transcription antitermination) regulon

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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