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NCBI: 06-JUL-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_1110 [new locus tag: NWMN_RS06275 ]
  • pan locus tag?: SAUPAN003479000
  • symbol: pyrP
  • pan gene symbol?: pyrP
  • synonym:
  • product: uracil permease

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_1110 [new locus tag: NWMN_RS06275 ]
  • symbol: pyrP
  • product: uracil permease
  • replicon: chromosome
  • strand: +
  • coordinates: 1214304..1215611
  • length: 1308
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGCAAAATGATGAAATGTTTGAACGAACAGTAAAACCCGTACTAGATGTAAATGAAAAA
    CCACAACCAGCGCAATGGGCATTTTTAAGCTTACAACATTTATTTGCGATGTTTGGCGCA
    ACAGTACTAGTACCATTCTTAACAGGACTACCAATATCAGCAGCGTTACTAGCTTCGGGA
    ATCGGTACATTACTTTATATCTTAATAACGAAGGCGCAAATACCAGCATACTTGGGATCT
    AGCTTTGCATTTATCACGCCAATTATCACGGGATTAAGTACGCATAGCTTAGGAGACATG
    CTTGTAGCATTATTCATGAGTGGTGTTATGTACGTCATCATCGGGATTCTAATCAAATTA
    AGTGGGACAGCATGGTTAATGAAATTATTACCACCAGTTGTTGTTGGACCAGTCATCATG
    GTAATTGGTTTAAGCTTAGCGCCTACTGCAGTCAATATGGCAATGTATGAAAATCCTGGA
    GATATGAAAGGTTACAATATCAGTTTCTTAATTGTTGCAATGATAACCTTGCTTGTAACA
    ATAGTCGTTCAAGGATTTTTTAAAGGATTCCTATCTTTAATTCCAGTTCTTGTAGGTATT
    ATCGTAGGTTATGTGGTAGCGATTTTTATGGGGATTGTGAAATTTGATGCAATTATGTCA
    GCAAAATGGATAGATTTCCCTCATATTTATCTGCCATTTAAAGATTATGTACCTTCATTT
    CACTTAGGACTTGTACTTGTAATGATTCCGATTGTGTTCGTAACAGTAAGTGAACATATT
    GGGCACCAAATGGTATTGAATAAAATCGTAGGTAGAAACTTCTTTGAAAAGCCAGGACTT
    GATAAATCAATCATTGGTGATGGTGTTTCTACAATGTTTGCCAGTATTATTGGTGGACCA
    CCAAGTACAACATACGGTGAAAATATCGGTGTATTAGCGATTACCAGAATATACAGTATT
    TACGTCATTGGTGGTGCAGCAGTTATAGCAATTGTTTTAGCATTCATTGGTAAGTTCACT
    GCATTAATTTCTTCTATACCTACACCAGTTATGGGAGGAGTATCTATATTACTTTTCGGT
    ATTATTGCAGCAAGTGGCTTAAGAATGTTAGTTGAAAGCAAAGTAGATTTTGCGAACAAT
    CGAAATTTAGTTATAGCTTCTGTAATTTTAGTTGTAGGTATCGGTAATTTAGTATTTAAC
    TTAAAAGAAATTGGTATCAACCTTCAAATTGAGGGGATGGCATTAGCTGCACTTTCAGGA
    ATTATTTTGAACTTAATCTTACCTAAAGAGAAAAAACAAAACAATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1308

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_1110 [new locus tag: NWMN_RS06275 ]
  • symbol: PyrP
  • description: uracil permease
  • length: 435
  • theoretical pI: 9.31816
  • theoretical MW: 46609.9
  • GRAVY: 0.991724

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Nucleosides, purines and pyrimidines uracil-xanthine permease (TIGR00801; HMM-score: 436.2)
    and 2 more
    pyrimidine utilization transport protein G (TIGR03616; HMM-score: 277.1)
    xanthine permease (TIGR03173; HMM-score: 240.6)
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    APC (CL0062) Xan_ur_permease; Permease family (PF00860; HMM-score: 361.2)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 12
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -0.5
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.009248
    • TAT(Tat/SPI): 0.000698
    • LIPO(Sec/SPII): 0.003138
  • predicted transmembrane helices (TMHMM): 11

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MQNDEMFERTVKPVLDVNEKPQPAQWAFLSLQHLFAMFGATVLVPFLTGLPISAALLASGIGTLLYILITKAQIPAYLGSSFAFITPIITGLSTHSLGDMLVALFMSGVMYVIIGILIKLSGTAWLMKLLPPVVVGPVIMVIGLSLAPTAVNMAMYENPGDMKGYNISFLIVAMITLLVTIVVQGFFKGFLSLIPVLVGIIVGYVVAIFMGIVKFDAIMSAKWIDFPHIYLPFKDYVPSFHLGLVLVMIPIVFVTVSEHIGHQMVLNKIVGRNFFEKPGLDKSIIGDGVSTMFASIIGGPPSTTYGENIGVLAITRIYSIYVIGGAAVIAIVLAFIGKFTALISSIPTPVMGGVSILLFGIIAASGLRMLVESKVDFANNRNLVIASVILVVGIGNLVFNLKEIGINLQIEGMALAALSGIILNLILPKEKKQNN

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: pyrR leader (transcription antitermination) regulon
    pyrR leader(RNA)important in Pyrimidine metabolism; regulatory site identified based on RegPrecise data for N315 RegPrecise binding of the PryR antitermination protein

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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