PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_1092 [new locus tag: SAUSA300_RS05920 ]
• pan locus tag^?: SAUPAN003479000
• symbol: pyrP
• pan gene symbol^?: pyrP
• synonym:
• product: uracil permease

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_1092 [new locus tag: SAUSA300_RS05920 ]
• symbol: pyrP
• product: uracil permease
• replicon: chromosome
• strand: +
• coordinates: 1194293..1195600
• length: 1308
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3914365 NCBI
• RefSeq: YP_493789 NCBI
• BioCyc: see SAUSA300_RS05920
• MicrobesOnline: 1292607 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

• 1
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  ATGCAAAATGATGAAATGTTTGAACGAACAGTAAAACCCGTACTAGATGTAAATGAAAAA
  CCACAACCAGCGCAATGGGCATTTTTAAGCTTACAACATTTATTTGCGATGTTTGGCGCA
  ACAGTACTAGTACCATTCTTAACAGGACTACCAATATCAGCAGCGTTACTAGCTTCGGGA
  ATCGGTACATTACTTTATATCTTAATAACGAAGGCGCAAATACCAGCATACTTGGGATCT
  AGCTTTGCATTTATCACGCCAATTATCACGGGATTAAGTACGCATAGCTTAGGAGACATG
  CTTGTAGCATTATTCATGAGTGGTGTTATGTACGTCATCATCGGGATTCTAATCAAATTA
  AGTGGGACAGCATGGTTAATGAAATTATTACCACCAGTTGTTGTTGGACCAGTCATCATG
  GTAATTGGTTTAAGCTTAGCGCCTACTGCAGTCAATATGGCAATGTATGAAAATCCTGGA
  GATATGAAAGGTTACAATATCAGTTTCTTAATTGTTGCAATGATAACCTTGCTTGTAACA
  ATAGTCGTTCAAGGATTTTTTAAAGGATTCCTATCTTTAATTCCAGTTCTTGTAGGTATT
  ATCGTAGGTTATGTGGTAGCGATTTTTATGGGGATTGTGAAATTTGATGCAATTATGTCA
  GCAAAATGGATAGATTTCCCTCATATTTATCTGCCATTTAAAGATTATGTACCTTCATTT
  CACTTAGGACTTGTACTTGTAATGATTCCGATTGTGTTCGTAACAGTAAGTGAACATATT
  GGGCACCAAATGGTATTGAATAAAATCGTAGGTAGAAACTTCTTTGAAAAGCCAGGACTT
  GATAAATCAATCATTGGTGATGGTGTTTCTACAATGTTTGCCAGTATTATTGGTGGACCA
  CCAAGTACAACATACGGTGAAAATATCGGTGTATTAGCGATTACCAGAATATACAGTATT
  TACGTCATTGGTGGTGCAGCAGTTATAGCAATTGTTTTAGCATTCATTGGTAAGTTCACT
  GCATTAATTTCTTCTATACCTACACCAGTTATGGGAGGAGTATCTATATTACTTTTCGGT
  ATTATTGCAGCAAGTGGCTTAAGAATGTTAGTTGAAAGCAAAGTAGATTTTGCGAACAAT
  CGAAATTTAGTTATAGCTTCTGTAATTTTAGTTGTAGGTATCGGTAATTTAGTATTTAAC
  TTAAAAGAAATTGGTATCAACCTTCAAATTGAGGGGATGGCATTAGCTGCACTTTCAGGA
  ATTATTTTGAACTTAATCTTACCTAAAGAGAAAAAACAAAACAATTAA
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⊟Protein

⊟General

• locus tag: SAUSA300_1092 [new locus tag: SAUSA300_RS05920 ]
• symbol: PyrP
• description: uracil permease
• length: 435
• theoretical pI: 9.31816
• theoretical MW: 46609.9
• GRAVY: 0.991724

⊟Function

• TIGRFAM:
  Metabolism Transport and binding proteins Nucleosides, purines and pyrimidines uracil-xanthine permease (TIGR00801; HMM-score: 436.2)
  and 2 more
  pyrimidine utilization transport protein G (TIGR03616; HMM-score: 277.1)
  xanthine permease (TIGR03173; HMM-score: 240.6)
• TheSEED  :
  • Uracil permease
  • Uracil:proton symporter UraA
  Nucleosides and Nucleotides Pyrimidines De Novo Pyrimidine Synthesis  Uracil permease
• PFAM:
  APC (CL0062) Xan_ur_permease; Permease family (PF00860; HMM-score: 361.2)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 12
• LocateP: Multi-transmembrane
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: -0.5
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.009248
  • TAT(Tat/SPI): 0.000698
  • LIPO(Sec/SPII): 0.003138
• predicted transmembrane helices (TMHMM): 11

⊟Accession numbers

• GI: 87160927 NCBI
• RefSeq: YP_493789 NCBI
• UniProt: A0A0H2XFJ8 UniProt

⊟Protein sequence

• MQNDEMFERTVKPVLDVNEKPQPAQWAFLSLQHLFAMFGATVLVPFLTGLPISAALLASGIGTLLYILITKAQIPAYLGSSFAFITPIITGLSTHSLGDMLVALFMSGVMYVIIGILIKLSGTAWLMKLLPPVVVGPVIMVIGLSLAPTAVNMAMYENPGDMKGYNISFLIVAMITLLVTIVVQGFFKGFLSLIPVLVGIIVGYVVAIFMGIVKFDAIMSAKWIDFPHIYLPFKDYVPSFHLGLVLVMIPIVFVTVSEHIGHQMVLNKIVGRNFFEKPGLDKSIIGDGVSTMFASIIGGPPSTTYGENIGVLAITRIYSIYVIGGAAVIAIVLAFIGKFTALISSIPTPVMGGVSILLFGIIAASGLRMLVESKVDFANNRNLVIASVILVVGIGNLVFNLKEIGINLQIEGMALAALSGIILNLILPKEKKQNN

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: pyrP > pyrB > pyrC > carA > carB > pyrF > pyrE > SAUSA300_1099

⊟Regulation

• regulator: pyrR leader (transcription antitermination) regulon

  pyrR leader

  (RNA)

  important in Pyrimidine metabolism; regulatory site identified based on RegPrecise data for N315 RegPrecise binding of the PyrR antitermination protein

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications