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NCBI: 06-JUL-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_1111 [new locus tag: NWMN_RS06280 ]
  • pan locus tag?: SAUPAN003480000
  • symbol: pyrB
  • pan gene symbol?: pyrB
  • synonym:
  • product: aspartate carbamoyltransferase catalytic subunit

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_1111 [new locus tag: NWMN_RS06280 ]
  • symbol: pyrB
  • product: aspartate carbamoyltransferase catalytic subunit
  • replicon: chromosome
  • strand: +
  • coordinates: 1215639..1216520
  • length: 882
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGAATCATTTATTATCAATGGAACATTTATCTACAGATCAAATATACAAACTTATCCAA
    AAGGCAAGTCAATTTAAATCTGGTGAACGTCAACTACCAAACTTTGAAGGGAAATATGTC
    GCAAATTTATTCTTTGAAAATTCTACTCGAACAAAATGTAGTTTTGAAATGGCAGAACTT
    AAGCTAGGGTTAAAAACGATTAGCTTTGAAACATCAACATCATCTGTTTCAAAAGGTGAA
    TCTTTATATGACACATGTAAAACTTTAGAAAGTATTGGCTGTGATTTATTAGTCATTAGA
    CATCCGTTTAATAACTACTATGAAAAATTAGCGAATATTAACATCCCAATTGCGAATGCT
    GGTGATGGTAGTGGACAACATCCAACACAAAGTTTACTTGATTTAATGACGATATATGAA
    GAATATGGATATTTTGAAGGCTTGAATGTATTGATTTGTGGAGACATTAAAAATTCACGT
    GTCGCACGTAGTAATTACCATAGTTTAAAAGCATTAGGTGCAAACGTAATGTTTAATAGC
    CCAAATGCTTGGATTGATGATTCTTTAGAAGCACCTTATGTAAATATAGATGATGTTATA
    GAAACAGTAGATATAGTTATGTTATTAAGAATTCAACATGAAAGGCATGGGCTTGCAGAA
    GAAACTAGATTTGCAGCAGATGATTATCATCAAAAGCATGGCTTAAATGAAGTACGCTAT
    AACAAATTACAAGAACATGCTATTGTTATGCATCCGGCACCTGTGAATAGAGGAGTAGAA
    ATACAAAGCGATTTAGTAGAAGCTTCAAAATCAAGAATTTTTAAGCAAATGGAAAATGGC
    GTTTACTTAAGAATGGCAGTCATTGATGAATTATTAAAATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    882

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_1111 [new locus tag: NWMN_RS06280 ]
  • symbol: PyrB
  • description: aspartate carbamoyltransferase catalytic subunit
  • length: 293
  • theoretical pI: 5.76441
  • theoretical MW: 33257.5
  • GRAVY: -0.323549

⊟Function[edit | edit source]

  • reaction:
    EC 2.1.3.2?  ExPASy
    Aspartate carbamoyltransferase Carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate
  • TIGRFAM:
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Pyrimidine ribonucleotide biosynthesis aspartate carbamoyltransferase (TIGR00670; EC 2.1.3.2; HMM-score: 305)
    and 3 more
    Metabolism Amino acid biosynthesis Glutamate family ornithine carbamoyltransferase (TIGR00658; EC 2.1.3.3; HMM-score: 138.8)
    Metabolism Energy metabolism Amino acids and amines putrescine carbamoyltransferase (TIGR04384; EC 2.1.3.6; HMM-score: 70.8)
    knotted carbamoyltransferase YgeW (TIGR03316; EC 2.1.3.-; HMM-score: 26.3)
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    no clan defined OTCace_N; Aspartate/ornithine carbamoyltransferase, carbamoyl-P binding domain (PF02729; HMM-score: 149.4)
    and 2 more
    Asp-glut_race (CL0399) OTCace; Aspartate/ornithine carbamoyltransferase, Asp/Orn binding domain (PF00185; HMM-score: 70.1)
    NADP_Rossmann (CL0063) 2-Hacid_dh_C; D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain (PF02826; HMM-score: 13.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.004927
    • TAT(Tat/SPI): 0.000294
    • LIPO(Sec/SPII): 0.000561
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MNHLLSMEHLSTDQIYKLIQKASQFKSGERQLPNFEGKYVANLFFENSTRTKCSFEMAELKLGLKTISFETSTSSVSKGESLYDTCKTLESIGCDLLVIRHPFNNYYEKLANINIPIANAGDGSGQHPTQSLLDLMTIYEEYGYFEGLNVLICGDIKNSRVARSNYHSLKALGANVMFNSPNAWIDDSLEAPYVNIDDVIETVDIVMLLRIQHERHGLAEETRFAADDYHQKHGLNEVRYNKLQEHAIVMHPAPVNRGVEIQSDLVEASKSRIFKQMENGVYLRMAVIDELLK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: pyrR leader (transcription antitermination) regulon
    pyrR leader(RNA)important in Pyrimidine metabolism; transcription unit transferred from N315 data RegPrecise binding of the PryR antitermination protein

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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