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FunGene: 08-OCT-2024
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus JSNZ
- locus tag: JSNZ_001173
- pan locus tag?: SAUPAN003484000
- symbol: pyrF
- pan gene symbol?: pyrF
- synonym:
- product: orotidine-5'-phosphate decarboxylase
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: JSNZ_001173
- symbol: pyrF
- product: orotidine-5'-phosphate decarboxylase
- replicon: chromosome
- strand: +
- coordinates: 1176265..1176957
- length: 693
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Gene ID:
- RefSeq:
- BioCyc:
- MicrobesOnline:
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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661ATGAAAGATTTACCAATTATTGCATTAGATTTTGAATCAAAAGAAAAAGTAAATCAATTT
TTAGATTTATTTGATGAATCATTATTCGTAAAAGTAGGTATGGAACTTTTTTATCAAGAA
GGTCCTCAATTAATTAATGAGATAAAAGAAAGAGGCCATGATGTATTTTTAGATTTAAAA
TTGCATGATATTCCTAATACAGTAGGTAAGGCGATGGAAGGACTAGCTAAATTGAATGTT
GATCTGGTAAATGTTCATGCTGCTGGTGGCGTAAAAATGATGTCTGAGGCCATTAAAGGA
TTAAGAAAACATAATCAACATACAAAAATTATTGCAGTAACACAGCTTACGTCAACAACA
GAAGACATGTTACGACACGAACAAAATATACAAACATCGATTGAAGAGGCCGTTTTAAAT
TATGCCAAGTTAGCAAATGCAGCTGGTTTAGATGGCGTTGTTTGTTCACCTCTTGAAAGT
CGTATGTTGACTGAAAAGTTAGGTACATCATTTTTAAAAGTAACACCAGGTATTAGACCT
AAAGGTGCATCTCAAGATGACCAACACCGTATTACGACACCGGAAGAAGCAAGACAGCTT
GGTTCGACGCATATTGTAGTCGGTAGACCGATTACACAAAGTGAAAATCCAGTCGAAAGT
TATCATAAAATTAAAGAAAGTTGGTTAGTATAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: JSNZ_001173
- symbol: PyrF
- description: orotidine-5'-phosphate decarboxylase
- length: 230
- theoretical pI: 6.23964
- theoretical MW: 25633.2
- GRAVY: -0.292174
⊟Function[edit | edit source]
- reaction: EC 4.1.1.23? ExPASyOrotidine-5'-phosphate decarboxylase Orotidine 5'-phosphate = UMP + CO2
- TIGRFAM: Purines, pyrimidines, nucleosides, and nucleotides Pyrimidine ribonucleotide biosynthesis orotidine 5'-phosphate decarboxylase (TIGR01740; EC 4.1.1.23; HMM-score: 208.4)and 1 more3-hexulose-6-phosphate synthase (TIGR03128; EC 4.1.2.43; HMM-score: 22.7)
- TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
- PFAM: TIM_barrel (CL0036) OMPdecase; Orotidine 5'-phosphate decarboxylase / HUMPS family (PF00215; HMM-score: 221.7)and 2 moreno clan defined DUF2120; Uncharacterized protein conserved in archaea (DUF2120) (PF09893; HMM-score: 14)DUF3842; Domain of unknown function (DUF3842) (PF12953; HMM-score: 14)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 7.5
- Cytoplasmic Membrane Score: 1.15
- Cellwall Score: 0.62
- Extracellular Score: 0.73
- Internal Helices: 0
- DeepLocPro: Cytoplasmic
- Cytoplasmic Score: 0.9996
- Cytoplasmic Membrane Score: 0.0002
- Cell wall & surface Score: 0.0001
- Extracellular Score: 0.0001
- LocateP:
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.000682
- TAT(Tat/SPI): 0.000081
- LIPO(Sec/SPII): 0.000126
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
- GI:
- RefSeq:
- UniProt:
⊟Protein sequence[edit | edit source]
- MKDLPIIALDFESKEKVNQFLDLFDESLFVKVGMELFYQEGPQLINEIKERGHDVFLDLKLHDIPNTVGKAMEGLAKLNVDLVNVHAAGGVKMMSEAIKGLRKHNQHTKIIAVTQLTSTTEDMLRHEQNIQTSIEEAVLNYAKLANAAGLDGVVCSPLESRMLTEKLGTSFLKVTPGIRPKGASQDDQHRITTPEEARQLGSTHIVVGRPITQSENPVESYHKIKESWLV
⊟Experimental data[edit | edit source]
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
- Operon-mapper [1] : pyrR > JSNZ_001168 > JSNZ_001169 > JSNZ_001170 > JSNZ_001171 > carB > pyrF > pyrE > JSNZ_001175
⊟Regulation[edit | edit source]
- data available for N315
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You can add further information about the gene and protein here.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ Blanca Taboada, Karel Estrada, Ricardo Ciria, Enrique Merino
Operon-mapper: a web server for precise operon identification in bacterial and archaeal genomes.
Bioinformatics: 2018, 34(23);4118-4120
[PubMed:29931111] [WorldCat.org] [DOI] (I p)