PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

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⊟Summary

• organism: Staphylococcus aureus COL
locus tag: SACOL1653 [new locus tag: SACOL_RS08430 ]
pan locus tag^?: SAUPAN004183000
symbol: SACOL1653
pan gene symbol^?: —
• synonym:
• product: GTP-binding protein YqeH

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SACOL1653 [new locus tag: SACOL_RS08430 ]
• symbol: SACOL1653
• product: GTP-binding protein YqeH
• replicon: chromosome
• strand: -
• coordinates: 1682211..1683311
• length: 1101
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3236250 NCBI
• RefSeq: YP_186493 NCBI
• BioCyc: see SACOL_RS08430
• MicrobesOnline: 913102 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  TTGTCTGACATTTTAAAATGTATCGGTTGTGGTGCGCCACTTCAATCTGAAGATAAAAAT
  AAACCTGGTTTTGTACCAGAGCATAATATGTTTCGTGATGACGTGATTTGCAGACGTTGT
  TTCCGCTTGAAAAATTATAACGAAGTTCAAGATGTAGGATTAGAAAGTGAAGACTTTTTA
  AAATTATTATCAGGACTTGCGGATAAAAAGGGTATTGTCGTCAATGTCGTGGATGTATTT
  GACTTTGAAGGATCATTTATTAATGCAGTTAAACGTATTGTCGGAAACAAAAAAATCATT
  TTAGCAGCAAATAAATTGGATTTATTGCCGAAACAAATTAATAAACGTCGAGTTAAAGAA
  TGGTTAAAACGAACAGCAAGAAAATATGGTTTGGAAGCTGACGATGTCGTATTAATTTCA
  GCTGAAAAAGGCTGGGGCATAGACGACTTATTATCATCAATTGCGAATATTCGAGAAAAT
  GAAGATGTGTATATTGTAGGGACAACGAATGTTGGGAAATCTACATTGATTAATAAACTG
  ATTGAAGCTAGTGTTGGTGAAAAAGATGTAGTAACAACTTCAAGATTCCCTGGAACAACT
  TTAGATATGATAGATATTCCTTTAGATGAAACATCATTTATGTATGATACACCAGGTATT
  ATTCAAGATCACCAAATGACGCATTTAGTTAGTGAAAAAGAATTGAAAATTATTATGCCT
  AAGAAAGAAATAAAACAACGCGTATATCAATTAAATGAGGCGCAGACATTATTCTTCGGC
  GGTCTAGCGCGCATAGATTATGTATCAGGTGGTAAACGTCCGTTAGTTTGTTTCTTTTCT
  AATGACTTGAATATACATCGTACTAAAACGGAGAAGGCTAATGATTTATGGCGTAATCAA
  CTTGGCGATTTATTAACGCCACCTGGAAATCCACAAAATTTTGATCTTAATGAGGTAAAG
  GCTGTTAGACTTGAAACAGGCAAAGAGAAACGCGATGTTATGATCTCTGGTCTAGGCTTT
  ATAACTATAGGACCAGGGGCTAAAGTAATCGTTCGTGTTCCTAAAAATGTTGATGTTGTA
  TTAAGAAATTCTATTTTATAA
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⊟Protein

⊟General

• locus tag: SACOL1653 [new locus tag: SACOL_RS08430 ]
• symbol: SACOL1653
• description: GTP-binding protein YqeH
• length: 366
• theoretical pI: 8.44888
• theoretical MW: 41086.3
• GRAVY: -0.22459

⊟Function

• ⊞TIGRFAM:
  Genetic information processing Protein synthesis Other ribosome biogenesis GTPase YqeH (TIGR03597; HMM-score: 486.9)
  and 19 more
  Genetic information processing Protein synthesis Other ribosome-associated GTPase EngA (TIGR03594; HMM-score: 60.6)
  Genetic information processing Protein synthesis Other ribosome biogenesis GTP-binding protein YlqF (TIGR03596; HMM-score: 52.5)
  Genetic information processing Protein synthesis Other ribosome biogenesis GTP-binding protein YsxC (TIGR03598; HMM-score: 40)
  Unknown function General GTP-binding protein HflX (TIGR03156; HMM-score: 37.5)
  Unknown function General small GTP-binding protein domain (TIGR00231; HMM-score: 37.4)
  Genetic information processing Protein synthesis Other GTP-binding protein Era (TIGR00436; HMM-score: 33.2)
  Genetic information processing Protein synthesis Other Obg family GTPase CgtA (TIGR02729; HMM-score: 32.9)
  Genetic information processing Protein fate Protein modification and repair [FeFe] hydrogenase H-cluster maturation GTPase HydF (TIGR03918; HMM-score: 30.5)
  Genetic information processing Protein synthesis Translation factors ribosome small subunit-dependent GTPase A (TIGR00157; EC 3.6.-.-; HMM-score: 28.5)
  Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA modification GTPase TrmE (TIGR00450; EC 3.6.-.-; HMM-score: 28.2)
  Metabolism Transport and binding proteins Cations and iron carrying compounds ferrous iron transport protein B (TIGR00437; HMM-score: 22.9)
  Genetic information processing Protein synthesis Translation factors selenocysteine-specific translation elongation factor (TIGR00475; HMM-score: 21.9)
  Unknown function General elongation factor 4 (TIGR01393; EC 3.6.5.-; HMM-score: 18.5)
  translation initiation factor 2, gamma subunit (TIGR03680; HMM-score: 18.4)
  Metabolism Energy metabolism Amino acids and amines ethanolamine utilization protein, EutP (TIGR02528; HMM-score: 15.3)
  Genetic information processing Protein synthesis Translation factors translation initiation factor IF-2 (TIGR00487; HMM-score: 14.2)
  Unknown function General GTP-binding protein YchF (TIGR00092; HMM-score: 13.9)
  Metabolism Transport and binding proteins Unknown substrate energy-coupling factor transporter ATPase (TIGR04521; EC 3.6.3.-; HMM-score: 11.7)
  Genetic information processing Protein fate Protein and peptide secretion and trafficking type VII secretion protein EccCb (TIGR03925; HMM-score: 11)
• TheSEED  :
  • GTP-binding protein YqeH, required for biogenesis of 30S ribosome subunit
  Protein Metabolism Protein biosynthesis Universal GTPases  GTP-binding protein YqeH, required for biogenesis of 30S ribosome subunit
• ⊞PFAM:
  no clan defined YqeH-like_C; YqeH-like, C-terminal (PF21516; HMM-score: 97)
  and 19 more
  P-loop_NTPase (CL0023) MMR_HSR1; 50S ribosome-binding GTPase (PF01926; HMM-score: 55.7)
  RsgA_GTPase; RsgA GTPase (PF03193; HMM-score: 39.7)
  FeoB_N; Ferrous iron transport protein B (PF02421; HMM-score: 34.4)
  GTP_EFTU; Elongation factor Tu GTP binding domain (PF00009; HMM-score: 31.3)
  Roc; Ras of Complex, Roc, domain of DAPkinase (PF08477; HMM-score: 20.9)
  Dynamin_N; Dynamin family (PF00350; HMM-score: 20.5)
  no clan defined MMR_HSR1_Xtn; C-terminal region of MMR_HSR1 domain (PF16897; HMM-score: 19)
  P-loop_NTPase (CL0023) Ras; Ras family (PF00071; HMM-score: 17.1)
  RNA_helicase; RNA helicase (PF00910; HMM-score: 14.9)
  ABC_tran; ABC transporter (PF00005; HMM-score: 14.5)
  ATPase_2; ATPase domain predominantly from Archaea (PF01637; HMM-score: 14.3)
  AAA_16; AAA ATPase domain (PF13191; HMM-score: 13.7)
  AAA_29; P-loop containing region of AAA domain (PF13555; HMM-score: 13.1)
  FtsK_SpoIIIE; FtsK/SpoIIIE family (PF01580; HMM-score: 13)
  no clan defined Swi5; Swi5 (PF07061; HMM-score: 12.5)
  P-loop_NTPase (CL0023) MobB; Molybdopterin guanine dinucleotide synthesis protein B (PF03205; HMM-score: 12.3)
  G-alpha; G-protein alpha subunit (PF00503; HMM-score: 12.1)
  MeaB; Methylmalonyl Co-A mutase-associated GTPase MeaB (PF03308; HMM-score: 11.2)
  TrwB_AAD_bind; Type IV secretion-system coupling protein DNA-binding domain (PF10412; HMM-score: 11.1)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• ⊞PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• ⊞DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.9293
  • Cytoplasmic Membrane Score: 0.0633
  • Cell wall & surface Score: 0.0001
  • Extracellular Score: 0.0072
• ⊞LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• ⊞SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.005254
  • TAT(Tat/SPI): 0.000227
  • LIPO(Sec/SPII): 0.000589
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 57651989 NCBI
• RefSeq: YP_186493 NCBI
• UniProt: A0A0H2WZ95 UniProt

⊟Protein sequence

• MSDILKCIGCGAPLQSEDKNKPGFVPEHNMFRDDVICRRCFRLKNYNEVQDVGLESEDFLKLLSGLADKKGIVVNVVDVFDFEGSFINAVKRIVGNKKIILAANKLDLLPKQINKRRVKEWLKRTARKYGLEADDVVLISAEKGWGIDDLLSSIANIRENEDVYIVGTTNVGKSTLINKLIEASVGEKDVVTTSRFPGTTLDMIDIPLDETSFMYDTPGIIQDHQMTHLVSEKELKIIMPKKEIKQRVYQLNEAQTLFFGGLARIDYVSGGKRPLVCFFSNDLNIHRTKTEKANDLWRNQLGDLLTPPGNPQNFDLNEVKAVRLETGKEKRDVMISGLGFITIGPGAKVIVRVPKNVDVVLRNSIL

⊟Experimental data

• experimentally validated: PeptideAtlas
• protein localization: Cytoplasmic
• quantitative data / protein copy number per cell: 86
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: comEA < SACOL1647 < SACOL1648 < SACOL1649 < nadD < SACOL1651 < aroE < SACOL1653 < SACOL1654 < mtn

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications