PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

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⊟Summary

• organism: Staphylococcus aureus N315
locus tag: SA1308 [new locus tag: SA_RS07400 ]
pan locus tag^?: SAUPAN003937000
symbol: rpsA
pan gene symbol^?: rpsA
• synonym:
• product: 30S ribosomal protein S1

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA1308 [new locus tag: SA_RS07400 ]
• symbol: rpsA
• product: 30S ribosomal protein S1
• replicon: chromosome
• strand: -
• coordinates: 1513700..1514875
• length: 1176
• essential: no DEG other strains

⊟Accession numbers

• Gene ID: 1124147 NCBI
• RefSeq: NP_374590 NCBI
• BioCyc: see SA_RS07400
• MicrobesOnline: 103616 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGACTGAAGAATTCAATGAATCAATGATTAACGATATTAAAGAAGGTGACAAAGTCACT
  GGCGAGGTACAACAAGTTGAAGACAAGCAAGTTGTTGTTCATATCAACGGTGGTAAATTT
  AATGGGATTATTCCTATTAGTCAACTATCTACGCATCATATTGATAGCCCAAGTGAAGTT
  GTAAAAGAGGGCGACGAAGTTGAAGCATATGTCACTAAAGTTGAGTTTGATGAAGAAAAT
  GAAACTGGAGCTTACATCTTATCTAGAAGACAACTTGAAACTGAGAAGTCTTATAGTTAT
  TTACAAGAAAAATTAGATAATAATGAAATCATCGAAGCGAAAGTAACAGAAGTAGTTAAA
  GGTGGTTTGGTTGTTGATGTAGGACAAAGAGGTTTTGTTCCGGCTTCACTAATTTCAACA
  GACTTCATTGAGGATTTCTCTGTGTTTGATGGACAAACAATTCGTATTAAAGTTGAAGAA
  TTGGATCCTGAAAATAATAGAGTCATTTTAAGCCGTAAAGCAGTTGAACAAGAAGAAAAC
  GATGCTAAAAAAGATCAATTATTACAATCTTTAAATGAAGGCGATGTTATTCATGGTAAA
  GTAGCGCGTTTAACTCAATTTGGTGCATTTATAGACATTGGTGGTGTTGATGGTTTAGTG
  CATGTATCTGAACTTTCTCACGAACATGTTCAAACACCAGAAGAAGTAGTTTCAATTGGT
  CAAGATGTTAAAGTTAAAATTAAATCTATTGATAGAGATACAGAACGTATTTCATTATCA
  ATCAAAGATACGTTACCAACACCTTTCGAAAATATTAAAGGTCAATTCCACGAAAATGAT
  GTCATTGAAGGTGTCGTAGTAAGATTGGCAAACTTTGGTGCATTTGTTGAAATTGCACCA
  GGTGTACAAGGACTTGTACATATTTCTGAAATTGCACACAAACACATTGGTACGCCAGGT
  GAAGTGTTAGAACCTGGTCAACAAGTAAATGTTAAAATATTAGGTATTGATGAAGAGAAT
  GAAAGAGTATCACTATCTATTAAAGCAACATTACCAAACGAAGATGTTGTTGAAAGTGAT
  CCTTCTACGACTAAGGCGTACTTAGAAAACGAAGAAGAAGATAATCCAACAATTGGCGAT
  ATGATTGGTGATAAACTTAAAAATCTTAAACTATAA
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⊟Protein

⊟General

• locus tag: SA1308 [new locus tag: SA_RS07400 ]
• symbol: RpsA
• description: 30S ribosomal protein S1
• length: 391
• theoretical pI: 4.27604
• theoretical MW: 43309
• GRAVY: -0.38977

⊟Function

• ⊞TIGRFAM:
  Genetic information processing Protein synthesis Ribosomal proteins: synthesis and modification ribosomal protein bS1 (TIGR00717; HMM-score: 286.7)
  and 8 more
  Genetic information processing Transcription Degradation of RNA polyribonucleotide nucleotidyltransferase (TIGR03591; EC 2.7.7.8; HMM-score: 147.6)
  guanosine pentaphosphate synthetase I/polyribonucleotide nucleotidyltransferase (TIGR02696; EC 2.7.-.-,2.7.7.8; HMM-score: 59.4)
  Genetic information processing Transcription DNA-dependent RNA polymerase DNA-directed RNA polymerase (TIGR00448; EC 2.7.7.6; HMM-score: 44)
  Genetic information processing Transcription Degradation of RNA ribonuclease R (TIGR02063; EC 3.1.-.-; HMM-score: 37)
  MAST domain (TIGR04204; HMM-score: 14.1)
  Metabolism Energy metabolism Glycolysis/gluconeogenesis carbon storage regulator (TIGR00202; HMM-score: 9.8)
  Signal transduction Regulatory functions RNA interactions carbon storage regulator (TIGR00202; HMM-score: 9.8)
  Genetic information processing Protein fate Protein and peptide secretion and trafficking preprotein translocase, YajC subunit (TIGR00739; HMM-score: 9.6)
• TheSEED  :
  • SSU ribosomal protein S1p
  Protein Metabolism Protein biosynthesis Ribosome SSU bacterial  SSU ribosomal protein S1p
• ⊞PFAM:
  OB (CL0021) S1; S1 RNA binding domain (PF00575; HMM-score: 252.4)
  and 13 more
  S1_RRP5; RRP5 S1 domain (PF23459; HMM-score: 94.8)
  Spt6_S1; Spt6, S1/OB domain (PF21710; HMM-score: 38.5)
  OB_RRP5_4th; RRP5 OB-fold domain (PF24685; HMM-score: 27.1)
  S1_2; S1 domain (PF13509; HMM-score: 19.2)
  RNase_II_C_S1; RNase II-type exonuclease C-terminal S1 domain (PF18614; HMM-score: 17.5)
  CusF_Ec; Copper binding periplasmic protein CusF (PF11604; HMM-score: 17.4)
  RuvA_N; RuvA N terminal domain (PF01330; HMM-score: 13.8)
  EXOSC1; Exosome component EXOSC1/CSL4 (PF10447; HMM-score: 11.3)
  no clan defined DUF1967; Domain of unknown function (DUF1967) (PF09269; HMM-score: 10.8)
  TPR (CL0020) COG4_N; Conserved oligomeric Golgi complex subunit 4, N-terminal (PF20663; HMM-score: 10.1)
  SH3 (CL0010) KOW; KOW motif (PF00467; HMM-score: 10)
  BPL_C; Biotin protein ligase C terminal domain (PF02237; HMM-score: 9.3)
  no clan defined CsrA; Global regulator protein family (PF02599; HMM-score: 7.9)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• ⊞PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• ⊞DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.9943
  • Cytoplasmic Membrane Score: 0.0022
  • Cell wall & surface Score: 0.0002
  • Extracellular Score: 0.0032
• ⊞LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• ⊞SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.021822
  • TAT(Tat/SPI): 0.000535
  • LIPO(Sec/SPII): 0.000886
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 15927057 NCBI
• RefSeq: NP_374590 NCBI
• UniProt: Q7A5J0 UniProt

⊟Protein sequence

• MTEEFNESMINDIKEGDKVTGEVQQVEDKQVVVHINGGKFNGIIPISQLSTHHIDSPSEVVKEGDEVEAYVTKVEFDEENETGAYILSRRQLETEKSYSYLQEKLDNNEIIEAKVTEVVKGGLVVDVGQRGFVPASLISTDFIEDFSVFDGQTIRIKVEELDPENNRVILSRKAVEQEENDAKKDQLLQSLNEGDVIHGKVARLTQFGAFIDIGGVDGLVHVSELSHEHVQTPEEVVSIGQDVKVKIKSIDRDTERISLSIKDTLPTPFENIKGQFHENDVIEGVVVRLANFGAFVEIAPGVQGLVHISEIAHKHIGTPGEVLEPGQQVNVKILGIDEENERVSLSIKATLPNEDVVESDPSTTKAYLENEEEDNPTIGDMIGDKLKNLKL

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications

Alexander Scherl, Patrice François, Manuela Bento, Jacques M Deshusses, Yvan Charbonnier, Véronique Converset, Antoine Huyghe, Nadia Walter, Christine Hoogland, Ron D Appel, Jean-Charles Sanchez, Catherine G Zimmermann-Ivol, Garry L Corthals, Denis F Hochstrasser, Jacques Schrenzel
Correlation of proteomic and transcriptomic profiles of Staphylococcus aureus during the post-exponential phase of growth.
J Microbiol Methods: 2005, 60(2);247-57
[PubMed:15590099] [WorldCat.org] [DOI] (P p)