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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_02285
  • pan locus tag?: SAUPAN005307000
  • symbol: SAOUHSC_02285
  • pan gene symbol?: leuA
  • synonym:
  • product: 2-isopropylmalate synthase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_02285
  • symbol: SAOUHSC_02285
  • product: 2-isopropylmalate synthase
  • replicon: chromosome
  • strand: +
  • coordinates: 2116716..2118245
  • length: 1530
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGAGTAGTCATATTCAAATTTTTGATACGACACTAAGAGACGGTGAACAAACACCAGGA
    GTGAATTTTACTTTTGATGAACGCTTGCGTATTGCATTGCAATTAGAAAAATGGGGTGTA
    GATGTTATTGAAGCTGGATTTCCTGCTTCAAGTACAGGTAGCTTTAAATCTGTTCAAGCA
    ATTGCACAAACATTAACAACAACGGCTGTATGTGGTTTAGCTAGATGTAAAAAATCTGAC
    ATCGATGCTGTATATGAAGCAACAAAAGATGCAGCGAAGCCGGTCGTGCATGTTTTTATA
    GCAACATCACCTATTCATCTTGAACATAAACTTAAAATGTCTCAAGAAGACGTTTTAGCA
    TCTATTAAAGAACATGTCACATACGCGAAACAATTATTTGACGTTGTTCAATTTTCACCT
    GAAGATGCAACGCGTACTGAATTACCATTCTTAGTGAAATGTGTACAAACTGCCGTTGAC
    GCTGGAGCTACAGTTATTAATATTCCTGATACAGTCGGCTACAGTTACCATGATGAATAT
    GCACATATTTTCAAAACCTTAACAGAATCTGTAACATCTTCAAATGAAATTATTTATAGT
    GCTCATTGCCATGACGATTTAGGAATGGCTGTTTCAAATAGTTTAGCTGCAATTGAAGGC
    GGTGCGAGACGAATTGAAGGCACTGTAAATGGTATTGGTGAACGAGCAGGTAATGCAGCA
    CTTGAAGAAGTCGCGCTTGCACTATACGTTCGAAATGATCATTATGGTGCTCAAACTGCT
    CTTAATCTCGAAGAAACTAAAAAAACATCGGATTTAATTTCAAGATATGCAGGTATTCGA
    GTGCCTAGAAATAAAGCAATTGTTGGCCAAAATGCATTTAGTCATGAATCAGGTATTCAC
    CAAGATGGCGTATTAAAACATCGTGAAACATATGAAATTATGACACCTCAACTTGTTGGT
    GTAAGCACGACTGAACTTCCATTAGGAAAATTATCTGGTAAACACGCCTTCTCAGAGAAG
    TTAAAAGCATTAGGTTATGACATTGATAAAGAAGCGCAAATAGATTTATTTAAACAATTC
    AAGGCCATTGCGGACAAAAAGAAATCTGTTTCAGATAGAGATATTCATGCGATTATTCAA
    GGTTCTGAGCATGAGCATCAAGCACTTTATAAATTGGAAACACTGCAACTACAATATGTC
    TCTAGCGGCCTTCAAAGTGCTGTTGTTGTTGTTAAAGATAAAGAGGGTCATATTTACCAG
    GATTCAAGTATTGGTACTGGTTCAATCGTAGCAATTTACAATGCAGTTGATCGTATTTTC
    CAGAAAGAAACAGAATTAATTGATTATCGTATTAATTCTGTCACTGAAGGTACTGATGCC
    CAAGCAGAAGTACATGTAAATTTATTGATTGAAGGTAAGACTGTCAATGGCTTTGGTATT
    GATCATGATATTTTACAAGCCTCTTGTAAAGCATACGTAGAAGCACATGCTAAATTTGCA
    GCTGAAAATGTTGAGAAGGTAGGTAATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1530

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_02285
  • symbol: SAOUHSC_02285
  • description: 2-isopropylmalate synthase
  • length: 509
  • theoretical pI: 5.75975
  • theoretical MW: 55674.3
  • GRAVY: -0.198821

⊟Function[edit | edit source]

  • ⊞reaction:
    EC 2.3.3.13?  ExPASy
    2-isopropylmalate synthase Acetyl-CoA + 3-methyl-2-oxobutanoate + H2O = (2S)-2-isopropylmalate + CoA
  • ⊞TIGRFAM:
    Metabolism Amino acid biosynthesis Pyruvate family 2-isopropylmalate synthase (TIGR00973; EC 2.3.3.13; HMM-score: 662.6)
    and 9 more
    isopropylmalate/citramalate/homocitrate synthases (TIGR02090; HMM-score: 405)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other homocitrate synthase (TIGR02660; EC 2.3.3.14; HMM-score: 315.2)
    Metabolism Central intermediary metabolism Nitrogen fixation homocitrate synthase (TIGR02660; EC 2.3.3.14; HMM-score: 315.2)
    homocitrate synthase (TIGR02146; EC 2.3.3.14; HMM-score: 282.4)
    Unknown function General citramalate synthase (TIGR00977; EC 2.3.1.182; HMM-score: 207.9)
    Metabolism Amino acid biosynthesis Pyruvate family 2-isopropylmalate synthase (TIGR00970; EC 2.3.3.13; HMM-score: 128)
    Metabolism Energy metabolism Other 4-hydroxy-2-oxovalerate aldolase (TIGR03217; EC 4.1.3.39; HMM-score: 75.6)
    Metabolism Transport and binding proteins Cations and iron carrying compounds oxaloacetate decarboxylase alpha subunit (TIGR01108; EC 4.1.1.3; HMM-score: 15.1)
    Metabolism Energy metabolism Other oxaloacetate decarboxylase alpha subunit (TIGR01108; EC 4.1.1.3; HMM-score: 15.1)
  • ⊞TheSEED  :
    • 2-isopropylmalate synthase (EC 2.3.3.13)
    Amino Acids and Derivatives Branched-chain amino acids Branched-Chain Amino Acid Biosynthesis  2-isopropylmalate synthase (EC 2.3.3.13)
    and 1 more
    Amino Acids and Derivatives Branched-chain amino acids Leucine Biosynthesis  2-isopropylmalate synthase (EC 2.3.3.13)
  • ⊞PFAM:
    TIM_barrel (CL0036) HMGL-like; HMGL-like (PF00682; HMM-score: 310.2)
    and 3 more
    no clan defined LeuA_dimer; LeuA allosteric (dimerisation) domain (PF08502; HMM-score: 126.3)
    Post-HMGL (CL0597) HCS_D2; Homocitrate synthase post-HMGL domain-like (PF22617; HMM-score: 95.8)
    ABC-2 (CL0181) ABC2_membrane_3; ABC-2 family transporter protein (PF12698; HMM-score: 10.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • ⊞DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9644
    • Cytoplasmic Membrane Score: 0.0124
    • Cell wall & surface Score: 0.0018
    • Extracellular Score: 0.0214
  • ⊞LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.005044
    • TAT(Tat/SPI): 0.002612
    • LIPO(Sec/SPII): 0.000366
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MSSHIQIFDTTLRDGEQTPGVNFTFDERLRIALQLEKWGVDVIEAGFPASSTGSFKSVQAIAQTLTTTAVCGLARCKKSDIDAVYEATKDAAKPVVHVFIATSPIHLEHKLKMSQEDVLASIKEHVTYAKQLFDVVQFSPEDATRTELPFLVKCVQTAVDAGATVINIPDTVGYSYHDEYAHIFKTLTESVTSSNEIIYSAHCHDDLGMAVSNSLAAIEGGARRIEGTVNGIGERAGNAALEEVALALYVRNDHYGAQTALNLEETKKTSDLISRYAGIRVPRNKAIVGQNAFSHESGIHQDGVLKHRETYEIMTPQLVGVSTTELPLGKLSGKHAFSEKLKALGYDIDKEAQIDLFKQFKAIADKKKSVSDRDIHAIIQGSEHEHQALYKLETLQLQYVSSGLQSAVVVVKDKEGHIYQDSSIGTGSIVAIYNAVDRIFQKETELIDYRINSVTEGTDAQAEVHVNLLIEGKTVNGFGIDHDILQASCKAYVEAHAKFAAENVEKVGN

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [1] [2]
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • ⊞regulator: CodY* (repression) regulon

⊟Transcription pattern[edit | edit source]

  • S.aureus Expression Data Browser:  [3] 
    Expression Data Browser
    ⊟Multi-gene expression profiles



    Click on any data point to display a description of the corresponding condition!

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. ↑ Jump up to: 3.0 3.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)
  4. ↑ Charlotte D Majerczyk, Paul M Dunman, Thanh T Luong, Chia Y Lee, Marat R Sadykov, Greg A Somerville, Kip Bodi, Abraham L Sonenshein
    Direct targets of CodY in Staphylococcus aureus.
    J Bacteriol: 2010, 192(11);2861-77
    [PubMed:20363936] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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