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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS08165 [old locus tag: SA1449 ]
  • pan locus tag?: SAUPAN004221000
  • symbol: SA_RS08165
  • pan gene symbol?: mnmA
  • synonym:
  • product: tRNA 2-thiouridine(34) synthase MnmA

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA_RS08165 [old locus tag: SA1449 ]
  • symbol: SA_RS08165
  • product: tRNA 2-thiouridine(34) synthase MnmA
  • replicon: chromosome
  • strand: -
  • coordinates: 1653329..1654447
  • length: 1119
  • essential: yes [1] DEG other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_002745 (1653329..1654447) NCBI
  • BioCyc: SA_RS08165 BioCyc
  • MicrobesOnline: see SA1449

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    TTGTCAAATAAAGATATACGTGTTGTCGTTGGTATGTCAGGCGGTGTAGATAGTTCTGTA
    ACAGCCCACGTCTTAAAAGAACAAGGTTATGATGTCATTGGCATATTTATGAAAAACTGG
    GATGACACTGACGAAAATGGCGTATGTACTGCAACTGAAGATTACAACGATGTTATTGAA
    GTGTGTAATCAAATTGGCATTCCATATTACGCTGTTAATTTTGAAAAAGAATATTGGGAT
    AAAGTCTTTACGTATTTCTTAGATGAATACAAAAAAGGTCGTACTCCAAATCCAGACGTT
    ATGTGTAATAAAGAAATTAAGTTTAAAGCCTTTTTAGATCATGCGATGAATTTAGGTGCA
    GATTATGTAGCAACAGGACATTACGCACGCATACATCGTCATGAAGATGGTCATGTTGAA
    ATGTTACGTGGTGTAGATAATAATAAAGATCAAACATACTTCTTGAATCAATTATCTCAA
    CAACAACTTTCAAAAGTGATGTTCCCAATTGGCGACATCGAAAAGAGTGAAGTGCGTCGA
    ATTGCTGAAGAACAAGGACTTGTTACTGCTAAGAAAAAAGATTCTACAGGCATTTGTTTT
    ATCGGCGAAAAAAACTTTAAAACATTTTTATCACAATATTTACCTGCACAACCGGGTGAT
    ATGATAACACTTGATGGTAAGAAAATGGGTAAACATAGTGGTTTGATGTATTACACAATA
    GGACAAAGACATGGATTAGGTATAGGTGGAGATGGCGATCCTTGGTTTGTTGTCGGTAAA
    AACCTAAAAGATAATGTTTTATATGTAGAACAAGGATTCCATCACGATGCATTATACAGT
    GATTACTTAATTGCTTCAGACTATTCATTTGTAAATCCAGAAGATAATGACTTAGATCAA
    GGTTTTGAATGTACAGCTAAATTTAGATATCGCCAAAAAGATACGAAAGTTTTTGTGAAA
    CGTGAAAACGACCATGCACTACGTGTTACTTTTGCTGAGCCAGTAAGAGCAATCACACCT
    GGACAAGCAGTTGTTTTTTATCAAGGTGATGTGTGTCTTGGTGGTGCAACAATTGACGAT
    GTGTTCAAAAATGAAGGTCAATTAAATTATGTTGTATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1119

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA_RS08165 [old locus tag: SA1449 ]
  • symbol: SA_RS08165
  • description: tRNA 2-thiouridine(34) synthase MnmA
  • length: 372
  • theoretical pI: 5.15955
  • theoretical MW: 42150.2
  • GRAVY: -0.457258

⊟Function[edit | edit source]

  • reaction:
    EC 2.8.1.13?  ExPASy
    tRNA-uridine 2-sulfurtransferase A [protein]-S-sulfanyl-L-cysteine + uridine34 in tRNA + ATP + reduced acceptor = a [protein]-L-cysteine + 2-thiouridine34 in tRNA + AMP + diphosphate + acceptor
  • TIGRFAM:
    Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase (TIGR00420; EC 2.1.1.61; HMM-score: 454.4)
    and 10 more
    Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA(Ile)-lysidine synthetase (TIGR02432; EC 6.3.4.-; HMM-score: 27.3)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pyridine nucleotides NAD+ synthetase (TIGR00552; EC 6.3.1.5; HMM-score: 24.8)
    Hypothetical proteins Conserved TIGR00268 family protein (TIGR00268; HMM-score: 22.3)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis GMP synthase (glutamine-hydrolyzing), C-terminal domain (TIGR00884; EC 6.3.5.2; HMM-score: 19.1)
    Unknown function General queuosine biosynthesis protein QueC (TIGR00364; HMM-score: 16.7)
    Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA sulfurtransferase ThiI (TIGR00342; EC 2.8.1.4; HMM-score: 15.8)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine tRNA sulfurtransferase ThiI (TIGR00342; EC 2.8.1.4; HMM-score: 15.8)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin putative heme d1 biosynthesis radical SAM protein NirJ2 (TIGR04055; EC 1.3.99.-; HMM-score: 15.1)
    Metabolism Amino acid biosynthesis Glutamate family argininosuccinate synthase (TIGR00032; EC 6.3.4.5; HMM-score: 14.8)
    Plasmodium falciparum CPW-WPC domain (TIGR01492; HMM-score: 11.8)
  • TheSEED: see SA1449
  • PFAM:
    HUP (CL0039) tRNA_Me_trans; tRNA methyl transferase (PF03054; HMM-score: 492.2)
    and 6 more
    NAD_synthase; NAD synthase (PF02540; HMM-score: 24.6)
    QueC; Queuosine biosynthesis protein QueC (PF06508; HMM-score: 20.7)
    ATP_bind_3; PP-loop family (PF01171; HMM-score: 20.3)
    Asn_synthase; Asparagine synthase (PF00733; HMM-score: 15.6)
    ThiI; Thiamine biosynthesis protein (ThiI) (PF02568; HMM-score: 15.6)
    TIKI (CL0572) Cofac_haem_bdg; Haem-binding uptake, Tiki superfamily, ChaN (PF04187; HMM-score: 12.6)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.02467
    • TAT(Tat/SPI): 0.000875
    • LIPO(Sec/SPII): 0.003451
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MSNKDIRVVVGMSGGVDSSVTAHVLKEQGYDVIGIFMKNWDDTDENGVCTATEDYNDVIEVCNQIGIPYYAVNFEKEYWDKVFTYFLDEYKKGRTPNPDVMCNKEIKFKAFLDHAMNLGADYVATGHYARIHRHEDGHVEMLRGVDNNKDQTYFLNQLSQQQLSKVMFPIGDIEKSEVRRIAEEQGLVTAKKKDSTGICFIGEKNFKTFLSQYLPAQPGDMITLDGKKMGKHSGLMYYTIGQRHGLGIGGDGDPWFVVGKNLKDNVLYVEQGFHHDALYSDYLIASDYSFVNPEDNDLDQGFECTAKFRYRQKDTKVFVKRENDHALRVTFAEPVRAITPGQAVVFYQGDVCLGGATIDDVFKNEGQLNYVV

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:
    SA_RS1168030S ribosomal protein S5  [2] (data from MRSA252)

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ R Allyn Forsyth, Robert J Haselbeck, Kari L Ohlsen, Robert T Yamamoto, Howard Xu, John D Trawick, Daniel Wall, Liangsu Wang, Vickie Brown-Driver, Jamie M Froelich, Kedar G C, Paula King, Melissa McCarthy, Cheryl Malone, Brian Misiner, David Robbins, Zehui Tan, Zhan-yang Zhu Zy, Grant Carr, Deborah A Mosca, Carlos Zamudio, J Gordon Foulkes, Judith W Zyskind
    A genome-wide strategy for the identification of essential genes in Staphylococcus aureus.
    Mol Microbiol: 2002, 43(6);1387-400
    [PubMed:11952893] [WorldCat.org] [DOI] (P p)
  2. ↑ Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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