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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_1578 [new locus tag: SAUSA300_RS08600 ]
  • pan locus tag?: SAUPAN004221000
  • symbol: mnmA
  • pan gene symbol?: mnmA
  • synonym:
  • product: tRNA-specific 2-thiouridylase MnmA

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_1578 [new locus tag: SAUSA300_RS08600 ]
  • symbol: mnmA
  • product: tRNA-specific 2-thiouridylase MnmA
  • replicon: chromosome
  • strand: -
  • coordinates: 1728638..1729723
  • length: 1086
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGTCAGGCGGTGTAGATAGTTCTGTAACAGCCCACGTCTTAAAAGAACAAGGTTATGAT
    GTCATTGGCATATTTATGAAAAACTGGGATGACACTGACGAAAATGGCGTATGTACTGCA
    ACTGAAGATTACAACGATGTTATTGAAGTGTGTAATCAAATTGGCATTCCGTATTACGCT
    GTTAATTTTGAAAAAGAATATTGGGATAAAGTCTTTACGTATTTCTTAGATGAATACAAA
    AAAGGTCGTACTCCAAATCCAGACGTTATGTGTAATAAAGAAATTAAGTTTAAAGCCTTT
    TTAGATCATGCGATGAATTTAGGTGCAGATTATGTAGCAACAGGACATTACGCACGCATA
    CATCGTCATGAAGATGGTCATGTTGAAATGTTACGTGGTGTAGATAATAATAAAGATCAA
    ACATACTTCTTGAATCAATTATCTCAACAACAACTTTCAAAAGTGATGTTCCCAATTGGC
    GACATCGAAAAGAGTGAAGTGCGTCGAATTGCTGAAGAACAAGGACTTGTTACTGCTAAG
    AAAAAAGATTCTACAGGCATTTGTTTTATCGGCGAAAAAAACTTTAAAACATTTTTATCA
    CAATATTTACCTGCACAACCGGGTGATATGATAACACTTGATGGTAAGAAAATGGGTAAA
    CATAGTGGTTTGATGTATTACACAATAGGACAAAGACATGGATTAGGTATAGGTGGAGAT
    GGCGATCCTTGGTTTGTTGTCGGTAAAAACCTAAAAGATAATGTTTTATATGTAGAACAA
    GGATTCCATCACGATGCATTATACAGTGATTACTTAATTGCTTCAGACTATTCATTTGTA
    AATCCAGAAGATAATGACTTAGATCAAGGTTTTGAATGTACAGCTAAATTTAGATATCGC
    CAAAAAGATACGAAAGTTTTTGTGAAACGTGAAAACGACCATGCACTACGTGTTACTTTT
    GCTGAGCCAGTAAGAGCAATCACACCTGGACAAGCAGTTGTTTTTTATCAAGGTGATGTG
    TGTCTTGGTGGTGCAACAATTGACGATGTGTTCAAAAATGAAGGTCAATTAAATTATGTT
    GTATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1086

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_1578 [new locus tag: SAUSA300_RS08600 ]
  • symbol: MnmA
  • description: tRNA-specific 2-thiouridylase MnmA
  • length: 361
  • theoretical pI: 5.04549
  • theoretical MW: 40950.7
  • GRAVY: -0.477839

⊟Function[edit | edit source]

  • reaction:
    EC 2.1.1.61?  ExPASy
    tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase S-adenosyl-L-methionine + tRNA containing 5-aminomethyl-2-thiouridine = S-adenosyl-L-homocysteine + tRNA containing 5-methylaminomethyl-2-thiouridylate
    EC 2.8.1.13?  ExPASy
    tRNA-uridine 2-sulfurtransferase A [protein]-S-sulfanyl-L-cysteine + uridine34 in tRNA + ATP + reduced acceptor = a [protein]-L-cysteine + 2-thiouridine34 in tRNA + AMP + diphosphate + acceptor
  • TIGRFAM:
    Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase (TIGR00420; EC 2.1.1.61; HMM-score: 445.5)
    and 8 more
    Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA(Ile)-lysidine synthetase (TIGR02432; EC 6.3.4.-; HMM-score: 20.7)
    Hypothetical proteins Conserved TIGR00268 family protein (TIGR00268; HMM-score: 16.9)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pyridine nucleotides NAD+ synthetase (TIGR00552; EC 6.3.1.5; HMM-score: 14.3)
    Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA sulfurtransferase ThiI (TIGR00342; EC 2.8.1.4; HMM-score: 13.3)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine tRNA sulfurtransferase ThiI (TIGR00342; EC 2.8.1.4; HMM-score: 13.3)
    Unknown function General queuosine biosynthesis protein QueC (TIGR00364; HMM-score: 12.9)
    Plasmodium falciparum CPW-WPC domain (TIGR01492; HMM-score: 12)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis GMP synthase (glutamine-hydrolyzing), C-terminal domain (TIGR00884; EC 6.3.5.2; HMM-score: 11.4)
  • TheSEED  :
    • tRNA-specific 2-thiouridylase MnmA (EC 2.8.1.13)
    Sulfur Metabolism Organic sulfur assimilation At5g37530 (CsdL protein family)  tRNA-specific 2-thiouridylase MnmA
  • PFAM:
    HUP (CL0039) tRNA_Me_trans; tRNA methyl transferase (PF03054; HMM-score: 483)
    and 6 more
    QueC; Queuosine biosynthesis protein QueC (PF06508; HMM-score: 16.1)
    NAD_synthase; NAD synthase (PF02540; HMM-score: 14)
    ATP_bind_3; PP-loop family (PF01171; HMM-score: 13.8)
    Asn_synthase; Asparagine synthase (PF00733; HMM-score: 12.8)
    ThiI; Thiamine biosynthesis protein (ThiI) (PF02568; HMM-score: 12.8)
    TIKI (CL0572) Cofac_haem_bdg; Haem-binding uptake, Tiki superfamily, ChaN (PF04187; HMM-score: 12.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.013326
    • TAT(Tat/SPI): 0.000337
    • LIPO(Sec/SPII): 0.00256
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MSGGVDSSVTAHVLKEQGYDVIGIFMKNWDDTDENGVCTATEDYNDVIEVCNQIGIPYYAVNFEKEYWDKVFTYFLDEYKKGRTPNPDVMCNKEIKFKAFLDHAMNLGADYVATGHYARIHRHEDGHVEMLRGVDNNKDQTYFLNQLSQQQLSKVMFPIGDIEKSEVRRIAEEQGLVTAKKKDSTGICFIGEKNFKTFLSQYLPAQPGDMITLDGKKMGKHSGLMYYTIGQRHGLGIGGDGDPWFVVGKNLKDNVLYVEQGFHHDALYSDYLIASDYSFVNPEDNDLDQGFECTAKFRYRQKDTKVFVKRENDHALRVTFAEPVRAITPGQAVVFYQGDVCLGGATIDDVFKNEGQLNYVV

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:
    SAUSA300_2187(rpsE)30S ribosomal protein S5  [1] (data from MRSA252)

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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