PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA_RS08005 [old locus tag: SA1418 ]
• pan locus tag^?: SAUPAN004176000
• symbol: SA_RS08005
• pan gene symbol^?: comEA
• synonym:
• product: competence protein ComE

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA_RS08005 [old locus tag: SA1418 ]
• symbol: SA_RS08005
• product: competence protein ComE
• replicon: chromosome
• strand: -
• coordinates: 1625096..1625782
• length: 687
• essential: no DEG other strains

⊟Accession numbers

• Location: NC_002745 (1625096..1625782) NCBI
• BioCyc: SA_RS08005 BioCyc
• MicrobesOnline: see SA1418

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  GTGGTTTTATTGTATCAATTTTTATTACGCTATAAAGATTTTTTAACTCAGTGGAAGTTA
  TATATTATAAGTGCTGTTGTTTTAATTATGGTATTAATTGGTTTTATATTCTGGAGACAA
  GATGATTATACTTCAAGAAATTTTGAAAATAAAGATACTGCTCTGAAACAAAGCACTAGT
  GAAAATAATAGTTTGTCCAAATTAGAAGATGTCCAGGTCAAAGATGGAGATAATTCCAAA
  AATAAAGGTCCTGTATATGTCGATGTAAAAGGTGCTGTTAAACATCCTAATGTTTATAAA
  ATGACATCTAAGGATAGAGTAGTTGATTTACTTGATAAAGCACAATTATTGGATGATGCA
  GATGTAAGTCGAATTAATTTGTCTGAAAAATTAACAGATCAAAAAATGATTTTCATACCT
  CATAAAGGACAAAAGAATGTTGAACCACAAATTGAAGTAAACAGTGTGCACGTAAAAAAT
  GGGAACACAAATAATACTAAAGTAAATTTAAATACCGCATCTGTATCAGAATTGATGTCT
  GTTCCTGGAGTAGGGCAAGCTAAAGCTAATGCAATTGTTGAATATCGCAACCAACAAGGT
  GCATTTCAAGAAATTGACGATTTGAAAAAAGTAAAAGGTTTTGGAAGTAAAACTTTTGAT
  AAACTGAAATCTTATTTCACGATATAA
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⊟Protein

⊟General

• locus tag: SA_RS08005 [old locus tag: SA1418 ]
• symbol: SA_RS08005
• description: competence protein ComE
• length: 228
• theoretical pI: 9.68419
• theoretical MW: 25900.5
• GRAVY: -0.421053

⊟Function

• TIGRFAM:
  Cellular processes Cellular processes DNA transformation comEA protein (TIGR01259; HMM-score: 115.6)
  and 4 more
  Cellular processes Cellular processes DNA transformation competence protein ComEA helix-hairpin-helix repeat region (TIGR00426; HMM-score: 74.3)
  Unknown function Enzymes of unknown specificity putative DNA modification/repair radical SAM protein (TIGR03916; HMM-score: 20.8)
  Metabolism Transport and binding proteins Nucleosides, purines and pyrimidines nucleoside Transporter (TIGR00939; HMM-score: 13.3)
  Genetic information processing DNA metabolism DNA replication, recombination, and repair Holliday junction DNA helicase RuvA (TIGR00084; EC 3.6.4.12; HMM-score: 13.2)
• TheSEED: see SA1418
• PFAM:
  HHH (CL0198) HHH_3; Helix-hairpin-helix motif (PF12836; HMM-score: 89.5)
  and 14 more
  HHH; Helix-hairpin-helix motif (PF00633; HMM-score: 47.7)
  HHH_5; Helix-hairpin-helix domain (PF14520; HMM-score: 33.4)
  T2SSK; Type II secretion system (T2SS), protein K (PF03934; HMM-score: 23.6)
  Ubiquitin (CL0072) SLBB; SLBB domain (PF10531; HMM-score: 23.4)
  HHH (CL0198) HHH_2; Helix-hairpin-helix motif (PF12826; HMM-score: 21.3)
  HHH_6; Helix-hairpin-helix motif (PF14579; HMM-score: 20.4)
  HHH_8; Helix-hairpin-helix domain (PF14716; HMM-score: 16.5)
  T2SSK_1st; Type II secretion system (T2SS), protein K (PF21687; HMM-score: 15.7)
  Aha1_BPI (CL0648) DUF1439; Protein of unknown function (DUF1439) (PF07273; HMM-score: 15.6)
  H2TH (CL0303) H2TH_5; S13-like H2TH domain (PF22525; HMM-score: 15)
  HHH (CL0198) UPF0758_N; UPF0758 N-terminal (PF20582; HMM-score: 14.9)
  PsbU; Photosystem II 12 kDa extrinsic protein (PsbU) (PF06514; HMM-score: 14.8)
  RNA_pol_A_CTD; Bacterial RNA polymerase, alpha chain C terminal domain (PF03118; HMM-score: 13.3)
  O-anti_assembly (CL0499) Wzy_C; O-Antigen ligase (PF04932; HMM-score: 11.8)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0.17
  • Cytoplasmic Membrane Score: 9.51
  • Cellwall Score: 0.16
  • Extracellular Score: 0.15
  • Internal Helix: 1
• DeepLocPro: Cytoplasmic Membrane
  • Cytoplasmic Score: 0.0002
  • Cytoplasmic Membrane Score: 0.9714
  • Cell wall & surface Score: 0.0016
  • Extracellular Score: 0.0268
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.001604
  • TAT(Tat/SPI): 0.000217
  • LIPO(Sec/SPII): 0.00129
• predicted transmembrane helices (TMHMM): 1

⊟Accession numbers

• GI: see SA1418
• RefSeq: WP_001802022 NCBI
• UniProt: see SA1418

⊟Protein sequence

• MVLLYQFLLRYKDFLTQWKLYIISAVVLIMVLIGFIFWRQDDYTSRNFENKDTALKQSTSENNSLSKLEDVQVKDGDNSKNKGPVYVDVKGAVKHPNVYKMTSKDRVVDLLDKAQLLDDADVSRINLSEKLTDQKMIFIPHKGQKNVEPQIEVNSVHVKNGNTNNTKVNLNTASVSELMSVPGVGQAKANAIVEYRNQQGAFQEIDDLKKVKGFGSKTFDKLKSYFTI

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: SigH* see SA1418

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications