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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS07865 [old locus tag: SA1391 ]
  • pan locus tag?: SAUPAN004143000
  • symbol: SA_RS07865
  • pan gene symbol?: dnaG
  • synonym:
  • product: DNA primase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA_RS07865 [old locus tag: SA1391 ]
  • symbol: SA_RS07865
  • product: DNA primase
  • replicon: chromosome
  • strand: -
  • coordinates: 1596711..1598528
  • length: 1818
  • essential: yes DEG other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_002745 (1596711..1598528) NCBI
  • BioCyc: SA_RS07865 BioCyc
  • MicrobesOnline: see SA1391

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    TTGCGAATAGATCAATCGATCATTAATGAAATAAAAGATAAAACCGACATTTTAGACTTG
    GTAAGTGAATATGTAAAACTAGAAAAGAGAGGACGCAATTATATAGGTTTGTGTCCTTTT
    CATGATGAAAAGACACCTTCATTTACAGTTTCTGAAGATAAACAAATCTGTCATTGTTTT
    GGTTGTAAAAAAGGTGGCAATGTTTTTCAATTTACTCAAGAAATTAAAGACATATCATTT
    GTTGAAGCGGTTAAAGAATTAGGTGATAGAGTTAATGTTGCTGTAGATATTGAGGCAACA
    CAATCTAACTCAAATGTTCAAATTGCTTCTGATGATTTACAAATGATTGAAATGCATGAG
    TTAATACAAGAATTTTATTATTACGCTTTAACAAAGACAGTCGAAGGCGAACAAGCATTA
    ACATACTTACAAGAACGTGGTTTTACAGATGCGCTTATTAAAGAGCGAGGCATTGGCTTT
    GCACCCGATAGCTCACATTTTTGTCATGATTTTCTTCAAAAAAAGGGTTACGATATTGAA
    TTAGCATATGAAGCCGGATTATTATCACGTAACGAAGAAAATTTCAGTTATTACGATAGA
    TTTCGAAATCGTATTATGTTTCCTTTGAAAAATGCGCAAGGAAGAATTGTTGGATATTCA
    GGTCGAACATATACCGGTCAAGAACCAAAATACCTAAATAGTCCTGAAACGCCTATCTTT
    CAAAAAAGAAAGTTGTTATATAACTTAGATAAAGCACGTAAATCAATTAGAAAATTAGAT
    GAAATTGTATTACTAGAAGGTTTTATGGATGTTATAAAATCTGATACTGCTGGCTTGAAA
    AACGTTGTTGCAACAATGGGTACACAGTTGTCAGATGAACATATTACCTTTATACGAAAG
    TTAACATCAAATATAACATTAATGTTTGATGGGGATTTTGCGGGTAGTGAAGCAACACTT
    AAAACAGGTCAACATTTGTTACAGCAAGGGCTAAATGTATTTGTTATACAATTGCCATCT
    GGCATGGATCCGGATGAATACATTGGTAAGTATGGCAACGACGCATTTACTACTTTTGTA
    AAAAATGACAAAAAGTCATTTGCACATTATAAAGTAAGTATATTAAAAGATGAAATTGCA
    CATAATGACCTTTCATATGAACGTTATTTGAAAGAACTGAGTCATGACATTTCACTTATG
    AAGTCATCAATTCTGCAACAAAAGGCTATAAATGATGTTGCGCCATTTTTCAATGTTAGT
    CCTGAGCAGTTAGCTAACGAAATACAATTCAATCAAGCACCAGCCAATTATTATCCAGAA
    GATGAGTATGGCGGTTATGATGAGTATGGCGGTTATATTGAACCTGAGCCAATTGGTATG
    GCACAATTTGACAATTTGAGCCGTCAAGAAAAAGCGGAGCGAGCATTTTTAAAACATTTA
    ATGAGAGATAAAGATACATTTTTAAATTATTATGAAAGTGTTGATAAGGATAACTTCACA
    AATCAGCATTTTAAATATGTATTCGAAGTCTTACATGATTTTTATGCGGAAAATGATCAA
    TATAATATCAGTGATGCTGTGCAGTATGTTAATTCAAATGAGTTGAGAGAAACACTAATT
    AGCTTAGAACAATATAATTTGAATGACGAACCATATGAAAATGAAATTGATGATTATGTC
    AATGTTATTAATGAAAAAGGACAAGAAACAATTGAGTCATTGAATCATAAATTAAGGGAA
    GCTACAAGGATTGGCGATGTAGAATTACAAAAATACTATTTACAGCAAATTGTTGCTAAG
    AATAAAGAACGCATGTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1818

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA_RS07865 [old locus tag: SA1391 ]
  • symbol: SA_RS07865
  • description: DNA primase
  • length: 605
  • theoretical pI: 4.69548
  • theoretical MW: 70031
  • GRAVY: -0.58562

⊟Function[edit | edit source]

  • reaction:
    EC 2.7.7.-?  ExPASy
  • TIGRFAM:
    Genetic information processing DNA metabolism DNA replication, recombination, and repair DNA primase (TIGR01391; EC 2.7.7.-; HMM-score: 507.9)
    and 4 more
    Unknown function General DNA primase-like protein (TIGR00646; HMM-score: 34.2)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair replicative DNA helicase (TIGR00665; EC 3.6.4.12; HMM-score: 21.1)
    Genetic information processing Transcription RNA processing ribonuclease M5 (TIGR00334; EC 3.1.26.8; HMM-score: 17.9)
    Genetic information processing Mobile and extrachromosomal element functions Prophage functions phage replicative helicase, DnaB family (TIGR03600; HMM-score: 13.2)
  • TheSEED: see SA1391
  • PFAM:
    DNA_prim_N (CL0748) DNAG_N; DNA primase catalytic core, N-terminal domain (PF08275; HMM-score: 142.3)
    Zn_Beta_Ribbon (CL0167) Zn_ribbon_DnaG; CHC2 zinc finger (PF01807; HMM-score: 131.1)
    no clan defined DnaG_cat_HB; DNA primase DnaG, catalytic core, helical bundle (PF21650; HMM-score: 116.8)
    and 6 more
    Toprim-like (CL0413) Toprim_2; Toprim-like (PF13155; HMM-score: 67.9)
    Toprim_4; Toprim domain (PF13662; HMM-score: 60.1)
    Toprim; Toprim domain (PF01751; HMM-score: 37)
    no clan defined DnaB; DnaB-like helicase N terminal domain (PF00772; HMM-score: 24.7)
    Toprim-like (CL0413) Toprim_3; Toprim domain (PF13362; HMM-score: 23.7)
    Globin (CL0090) HisK-N-like; HisK-N-like globin domain of the ASK signalosome (PF20302; HMM-score: 6.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: Mg2+, Zn2+
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.973
    • Cytoplasmic Membrane Score: 0.0124
    • Cell wall & surface Score: 0.0029
    • Extracellular Score: 0.0117
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.074085
    • TAT(Tat/SPI): 0.003323
    • LIPO(Sec/SPII): 0.00402
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MRIDQSIINEIKDKTDILDLVSEYVKLEKRGRNYIGLCPFHDEKTPSFTVSEDKQICHCFGCKKGGNVFQFTQEIKDISFVEAVKELGDRVNVAVDIEATQSNSNVQIASDDLQMIEMHELIQEFYYYALTKTVEGEQALTYLQERGFTDALIKERGIGFAPDSSHFCHDFLQKKGYDIELAYEAGLLSRNEENFSYYDRFRNRIMFPLKNAQGRIVGYSGRTYTGQEPKYLNSPETPIFQKRKLLYNLDKARKSIRKLDEIVLLEGFMDVIKSDTAGLKNVVATMGTQLSDEHITFIRKLTSNITLMFDGDFAGSEATLKTGQHLLQQGLNVFVIQLPSGMDPDEYIGKYGNDAFTTFVKNDKKSFAHYKVSILKDEIAHNDLSYERYLKELSHDISLMKSSILQQKAINDVAPFFNVSPEQLANEIQFNQAPANYYPEDEYGGYDEYGGYIEPEPIGMAQFDNLSRQEKAERAFLKHLMRDKDTFLNYYESVDKDNFTNQHFKYVFEVLHDFYAENDQYNISDAVQYVNSNELRETLISLEQYNLNDEPYENEIDDYVNVINEKGQETIESLNHKLREATRIGDVELQKYYLQQIVAKNKERM

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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