From AureoWiki
Jump to navigation Jump to search

NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS01315 [old locus tag: SA0224 ]
  • pan locus tag?: SAUPAN001093000
  • symbol: SA_RS01315
  • pan gene symbol?: fadB
  • synonym:
  • product: 3-hydroxyacyl-CoA dehydrogenase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA_RS01315 [old locus tag: SA0224 ]
  • symbol: SA_RS01315
  • product: 3-hydroxyacyl-CoA dehydrogenase
  • replicon: chromosome
  • strand: -
  • coordinates: 268453..270714
  • length: 2262
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_002745 (268453..270714) NCBI
  • BioCyc: SA_RS01315 BioCyc
  • MicrobesOnline: see SA0224

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGACAATTAATAAAGTAACCGTTCTTGGCGCAGGCACGATGGGCGCTCAACTGGCAGCA
    CTCTTTGTGAATGCTGGACTTAAAGTAAAACTATTAGATATTGTAGTGGACAAAAACGAT
    CCAAATCTCATTGCGAAAAAATCTTACGATAAAATTACAGATAAGAAACGGCCGCTACTA
    TTCGACTTAAATCTAGTGAGTCATTTAACATATGGTAATTTTGATGATGACTTGGTAAAT
    GATGATGCTGATTTATATATCGAAGCAGTCAAAGAAGATATTGAAATTAAGCATGCTGTT
    TGGCAACAAGTTCTACAACATGCTAAAGAAGATGCTTTATTTGCTACAAATACATCAGGT
    ATTCCAATTAATGCGATTGCTCAAGCATTTAACGAGAAGGATCAAGAACGATTCTTTGGT
    CTACATTTCTTTAACCCACCACGTATTATGAAATTAGTGGAGTTAATACCTACGTCACAC
    ACGAAGGAATCTATCATATTAGATGTAAAAAATTTCGCGCAAAATGTATTAGGTAAAGGT
    GTCATTGTCGTCAATGATGTGCCTGGCTTTGTCGCAAATAGAGTCGGCACGCAAACAATG
    AATGATATTATGTATCGCGCCGAGCAACACAAGTTAAGCATTGTAGATGTGGATGCTTTA
    ACTGGGCAAGCGATTGGTCGTCCTAAAACAGGTACATATGCCCTATCCGACCTAGTCGGT
    TTAGATATTGCAGTGTCTGTAATTAAAGGCATGCAACAAGTACCTGAAGAAACGCCTTAT
    TTTCATGATGTCAAAATTGTAAATACGTTGTTTGACAATGGCGCACTCGGACGTAAAACG
    AAACAAGGATTTTACAAAAAGGATAAAGAAACTAAAGCTCGACTTGTTTACGATGTTGAA
    AAACAAGATTATGTACCTGTATCGCAACCACAATTACCAATTTTAAATGAATTTAATAAA
    GACTTAGTGCATAACCTTGATGTCATATTCAATGCGCAAGACGAAGCGGGACTATTTTTA
    TGGGAGACATTACGTAATAATTTCTATTACTCTGCTATCAATGTACCTAAAGCTACCGAT
    GATTTCCGAGACATAGACCGTGCGCTTGTCTGGGGGTTCAACTGGAAACTTGGTCCATTC
    CAATTATGGGATGCAATGGGATACGAACGTGTTAAAACACGTATGGAAGACGAACTTGGA
    GACTTACCACAATGGATTAGTGATTTAGATGGTGGCTTTTATAAACAAGATGAGACCATT
    GAATATGCAACACCTGTTTCTCACTTCGTAAAAGATGAACTTTGGGATAAAGGTGATGCC
    AAACTTTCCGTAACTCATGATGATCAACTGTTACTGAAATTACAAAGTAAAAATAATGTC
    ATTACCGATGAATTCAACGATGCGTTAGTTGATGCCATTGATTTACTGGAAAATGACCAT
    TACACAAGTATGGTCATTTATGCAGATGGTAATAATTTCAGTGTGGGTGCTAACCTTTTC
    TTAATGAAAAAGGCGCATGAAGACGGTCTTGTAGATGATGTCGTTGCACAATCAATTGAT
    AAATTACATTATAGCTTTAATCGTTTGAAGTATAGTTTGAAACCAGTAGTCACAGCTGTT
    CAAGGTCGTGCCTTAGGCGGTGGCTGTGAGCTTGTACTTTACTCACCTATTGTTGTCGCT
    GCAAGTGAAACATATATAGGTCTTGTTGAAGCAGGCGTTGGCTTATTACCGAGTGGCGGT
    GGCCTTGCAGAAATGGCTGATCGCATATTACGCACATCGCATAAGTTTGATGACAAACAA
    GCTTCCATGACAAAAGTACTGACGAATATCGCATTTGCGAAAGTCTCTACAAATGCCTTT
    GAGGCACGTCGTTATGGTTATTTACGTGATACAGATACGATTATTTTCAATACAGCACAA
    CGTGTCGAAGTTGCGCTCAAACGTGCGAAATATGAAGCAGAAACAAACTATATTCCGAAT
    CCTAGACATCAATATATCGCTTTAGGTGAAGACTTCAAAGCATTGATCCAAGGACAATTA
    GATGCGCAAAGACGGGGGCATTTTATTAGCGACCATGATTATCATATCGCCTTAAATATC
    GCCACAATTTTAGCGGGTGGTGATTTACCAAGAAATACATTTATAAATCAACGTTACATT
    CAATCGTTGGAGAAAATTGGCTTTATTGACTTACTAAAATCTAAAAAATCATATGAAAGA
    ATTGCACATATGTTAAAAACTGGTAAGCCATTACGTAATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2160
    2220
    2262

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA_RS01315 [old locus tag: SA0224 ]
  • symbol: SA_RS01315
  • description: 3-hydroxyacyl-CoA dehydrogenase
  • length: 753
  • theoretical pI: 5.83805
  • theoretical MW: 84619.8
  • GRAVY: -0.234396

⊟Function[edit | edit source]

  • reaction:
    EC 1.1.1.35  ExPASy
    3-hydroxyacyl-CoA dehydrogenase (S)-3-hydroxyacyl-CoA + NAD+ = 3-oxoacyl-CoA + NADH?
  • TIGRFAM:
    fatty acid oxidation complex, alpha subunit, mitochondrial (TIGR02441; EC 1.1.1.35,4.2.1.17; HMM-score: 174.9)
    Metabolism Fatty acid and phospholipid metabolism Degradation fatty oxidation complex, alpha subunit FadB (TIGR02437; EC 1.1.1.35,4.2.1.17,5.1.2.3,5.3.3.8; HMM-score: 165.6)
    Metabolism Fatty acid and phospholipid metabolism Degradation fatty oxidation complex, alpha subunit FadJ (TIGR02440; EC 1.1.1.35,4.2.1.17,5.1.2.3; HMM-score: 152.4)
    and 10 more
    3-hydroxyacyl-CoA dehydrogenase PaaC (TIGR02279; EC 1.1.1.-; HMM-score: 134.4)
    phenylacetate degradation probable enoyl-CoA hydratase PaaB (TIGR02280; EC 4.2.1.17; HMM-score: 41.4)
    cyclohexa-1,5-dienecarbonyl-CoA hydratase (TIGR03189; EC 4.2.1.100; HMM-score: 28.9)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone naphthoate synthase (TIGR01929; EC 4.1.3.36; HMM-score: 17.9)
    2-ketocyclohexanecarboxyl-CoA hydrolase (TIGR03210; EC 3.7.-.-; HMM-score: 17.4)
    nucleotide sugar dehydrogenase (TIGR03026; HMM-score: 13.9)
    Metabolism Amino acid biosynthesis Glutamate family pyrroline-5-carboxylate reductase (TIGR00112; EC 1.5.1.2; HMM-score: 13.6)
    Metabolism Energy metabolism Amino acids and amines alanine dehydrogenase (TIGR00518; EC 1.4.1.1; HMM-score: 13.3)
    benzoyl-CoA-dihydrodiol lyase (TIGR03222; EC 4.1.2.44; HMM-score: 12.9)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Chlorophyll and bacteriochlorphyll geranylgeranyl reductase family (TIGR02032; EC 1.3.1.-; HMM-score: 12.1)
  • TheSEED: see SA0224
  • PFAM:
    NADP_Rossmann (CL0063) 3HCDH_N; 3-hydroxyacyl-CoA dehydrogenase, NAD binding domain (PF02737; HMM-score: 173.1)
    and 8 more
    6PGD_C (CL0106) 3HCDH; 3-hydroxyacyl-CoA dehydrogenase, C-terminal domain (PF00725; HMM-score: 93.4)
    ClpP_crotonase (CL0127) ECH_1; Enoyl-CoA hydratase/isomerase (PF00378; HMM-score: 60.3)
    ECH_2; Enoyl-CoA hydratase/isomerase (PF16113; HMM-score: 33.4)
    NADP_Rossmann (CL0063) NAD_binding_2; NAD binding domain of 6-phosphogluconate dehydrogenase (PF03446; HMM-score: 14.9)
    P-loop_NTPase (CL0023) CbiA; CobQ/CobB/MinD/ParA nucleotide binding domain (PF01656; HMM-score: 13.4)
    Zot; Zonular occludens toxin (Zot) (PF05707; HMM-score: 13.4)
    NADP_Rossmann (CL0063) NAD_binding_7; Putative NAD(P)-binding (PF13241; HMM-score: 12.7)
    NAD_Gly3P_dh_N; NAD-dependent glycerol-3-phosphate dehydrogenase N-terminus (PF01210; HMM-score: 12)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.120172
    • TAT(Tat/SPI): 0.008308
    • LIPO(Sec/SPII): 0.024802
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MTINKVTVLGAGTMGAQLAALFVNAGLKVKLLDIVVDKNDPNLIAKKSYDKITDKKRPLLFDLNLVSHLTYGNFDDDLVNDDADLYIEAVKEDIEIKHAVWQQVLQHAKEDALFATNTSGIPINAIAQAFNEKDQERFFGLHFFNPPRIMKLVELIPTSHTKESIILDVKNFAQNVLGKGVIVVNDVPGFVANRVGTQTMNDIMYRAEQHKLSIVDVDALTGQAIGRPKTGTYALSDLVGLDIAVSVIKGMQQVPEETPYFHDVKIVNTLFDNGALGRKTKQGFYKKDKETKARLVYDVEKQDYVPVSQPQLPILNEFNKDLVHNLDVIFNAQDEAGLFLWETLRNNFYYSAINVPKATDDFRDIDRALVWGFNWKLGPFQLWDAMGYERVKTRMEDELGDLPQWISDLDGGFYKQDETIEYATPVSHFVKDELWDKGDAKLSVTHDDQLLLKLQSKNNVITDEFNDALVDAIDLLENDHYTSMVIYADGNNFSVGANLFLMKKAHEDGLVDDVVAQSIDKLHYSFNRLKYSLKPVVTAVQGRALGGGCELVLYSPIVVAASETYIGLVEAGVGLLPSGGGLAEMADRILRTSHKFDDKQASMTKVLTNIAFAKVSTNAFEARRYGYLRDTDTIIFNTAQRVEVALKRAKYEAETNYIPNPRHQYIALGEDFKALIQGQLDAQRRGHFISDHDYHIALNIATILAGGDLPRNTFINQRYIQSLEKIGFIDLLKSKKSYERIAHMLKTGKPLRN

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:
    SA_RS04580cytosol aminopeptidase  [1] (data from MRSA252)
    SA_RS05350pyruvate dehydrogenase E1 component subunit alpha  [1] (data from MRSA252)
    SA_RS0829550S ribosomal protein L21  [1] (data from MRSA252)
    SA_RS08760formate--tetrahydrofolate ligase  [1] (data from MRSA252)
    SA_RS1174050S ribosomal protein L22  [1] (data from MRSA252)

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ 1.0 1.1 1.2 1.3 1.4 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

Retrieved from "http://fungenwikiserver.biologie.uni-greifswald.de/aureowiki/index.php?title=SA_RS01315&oldid=101581"