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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_RS09070 [old locus tag: SAUSA300_1662 ]
  • pan locus tag?: SAUPAN004368000
  • symbol: SAUSA300_RS09070
  • pan gene symbol?: nifZ
  • synonym:
  • product: cysteine desulfurase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_RS09070 [old locus tag: SAUSA300_1662 ]
  • symbol: SAUSA300_RS09070
  • product: cysteine desulfurase
  • replicon: chromosome
  • strand: -
  • coordinates: 1827324..1828472
  • length: 1149
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    GTGTTTATTTTGATATATCTAGATAATGCGGCAACGACGAAAGCATTTGAAGAAGTGTTA
    GATACTTATTTAAAAGTAAATCAATCAATGTATTATAATCCGAATAGTCCGCATAAAGCT
    GGTTTGCAGGCAAATCAATTACTACAACAAGCAAAAACCCAAATTAATGCAATGATTAAT
    TCAAAAACAAATTATGATGTTGTATTCACTAGTGGTGCAACTGAATCCAATAATCTTGCT
    TTAAAAGGTATTGCCTATCGTAAATTTGATACAGCGAAGGAAATAATTACATCCGTGTTA
    GAGCATCCGTCCGTATTAGAGGTTGTAAGATATTTGGAAGCACACGAAGGATTTAAAGTT
    AAATATGTTGATGTAAAGAAAGATGGCAGTATTAACTTAGAACACTTCAAAGAATTAATG
    TCAGACAAAGTCGGTTTAGTAACATGTATGTATGTAAATAATGTAACTGGACAAATACAG
    CCTATTCCACAAATGGCTAAAGTTATAAAAAATTATCCTAAGGCACATTTTCATGTAGAT
    GCGGTTCAAGCATTCGGCAAAATTTCAATGGATCTCAATAACATAGATAGTATTAGTTTA
    AGTGGACACAAGTTTAATGGTTTAAAAGGACAAGGCGTCTTACTTGTAAATCACATTCAA
    AATGTTGAACCAACTGTCCATGGTGGTGGTCAAGAATATGGCGTTAGAAGTGGAACAGTT
    AATTTGCCAAATGATATTGCAATGGTTAAAGCGATGAAGATAGCTAATGAAAACTTTGAA
    GCATTGAATGCATTTGTTACTGAGTTAAATAATGACGTCCGTCAATTTTTAAATAAATAT
    CATGGAGTTTATATTAATTCTTCAACTTCAGGTTCACCATTCGTTTTAAATATTAGTTTT
    CCTGGCGTAAAAGGTGAAGTATTAGTTAATGCTTTTTCAAAATATGACATTATGATATCT
    ACGACAAGTGCTTGTTCATCTAAACGTAATAAATTAAATGAAGTATTGGCTGCAATGGGA
    TTATCAGACAAATCTATTGAAGGTAGTATAAGATTATCATTTGGGGCTACTACAACTAAA
    GAAGATATAGCGAGGTTTAAAGAAATATTTATCATCATTTATGAGGAAATTAAGGAGTTG
    CTAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1149

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_RS09070 [old locus tag: SAUSA300_1662 ]
  • symbol: SAUSA300_RS09070
  • description: cysteine desulfurase
  • length: 382
  • theoretical pI: 8.1916
  • theoretical MW: 42365.3
  • GRAVY: -0.126178

⊟Function[edit | edit source]

  • TIGRFAM:
    cysteine desulfurase NifS (TIGR03402; EC 2.8.1.7; HMM-score: 310.8)
    Genetic information processing DNA metabolism Restriction/modification cysteine desulfurase DndA (TIGR03235; EC 2.8.1.7; HMM-score: 297.6)
    Genetic information processing Protein synthesis tRNA and rRNA base modification cysteine desulfurase IscS (TIGR02006; EC 2.8.1.7; HMM-score: 282.5)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other cysteine desulfurase IscS (TIGR02006; EC 2.8.1.7; HMM-score: 282.5)
    cysteine desulfurase, NifS family (TIGR03403; EC 2.8.1.7; HMM-score: 250.1)
    and 7 more
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other cysteine desulfurase, SufS family (TIGR01979; HMM-score: 140)
    Unknown function Enzymes of unknown specificity cysteine desulfurase family protein (TIGR01977; HMM-score: 132.1)
    Unknown function Enzymes of unknown specificity cysteine desulfurase family protein (TIGR01976; HMM-score: 86.2)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other cysteine desulfurase, catalytic subunit CsdA (TIGR03392; EC 2.8.1.7; HMM-score: 76.4)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Glutathione and analogs ergothioneine biosynthesis PLP-dependent enzyme EgtE (TIGR04343; EC 4.4.-.-; HMM-score: 43.9)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other tyrosine decarboxylase MnfA (TIGR03812; EC 4.1.1.25; HMM-score: 28)
    Metabolism Central intermediary metabolism Phosphorus compounds 2-aminoethylphosphonate--pyruvate transaminase (TIGR02326; EC 2.6.1.37; HMM-score: 25.4)
  • TheSEED: see SAUSA300_1662
  • PFAM:
    PLP_aminotran (CL0061) Aminotran_5; Aminotransferase class-V (PF00266; HMM-score: 236)
    and 4 more
    DegT_DnrJ_EryC1; DegT/DnrJ/EryC1/StrS aminotransferase family (PF01041; HMM-score: 20.6)
    EF_hand (CL0220) EF-hand_1; EF hand (PF00036; HMM-score: 15.8)
    PLP_aminotran (CL0061) Beta_elim_lyase; Beta-eliminating lyase (PF01212; HMM-score: 15.5)
    no clan defined DUF4473; Domain of unknown function (DUF4473) (PF14747; HMM-score: 8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: pyridoxal 5'-phosphate
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.67
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.15
    • Extracellular Score: 0.17
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.011201
    • TAT(Tat/SPI): 0.000784
    • LIPO(Sec/SPII): 0.00173
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MFILIYLDNAATTKAFEEVLDTYLKVNQSMYYNPNSPHKAGLQANQLLQQAKTQINAMINSKTNYDVVFTSGATESNNLALKGIAYRKFDTAKEIITSVLEHPSVLEVVRYLEAHEGFKVKYVDVKKDGSINLEHFKELMSDKVGLVTCMYVNNVTGQIQPIPQMAKVIKNYPKAHFHVDAVQAFGKISMDLNNIDSISLSGHKFNGLKGQGVLLVNHIQNVEPTVHGGGQEYGVRSGTVNLPNDIAMVKAMKIANENFEALNAFVTELNNDVRQFLNKYHGVYINSSTSGSPFVLNISFPGVKGEVLVNAFSKYDIMISTTSACSSKRNKLNEVLAAMGLSDKSIEGSIRLSFGATTTKEDIARFKEIFIIIYEEIKELLK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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