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NCBI: 26-AUG-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA1538 [new locus tag: SA_RS08655 ]
  • pan locus tag?: SAUPAN004368000
  • symbol: SA1538
  • pan gene symbol?: nifZ
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA1538 [new locus tag: SA_RS08655 ]
  • symbol: SA1538
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 1756869..1758008
  • length: 1140
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    TTGATATATCTAGATAATGCGGCAACGACGAAAGCATTTGAAGAAGTGTTAGATACTTAT
    TTAAAAGTAAATCAATCAATGTATTATAATCCGAATAGTCCGCATAAAGCTGGTTTGCAG
    GCAAATCAATTACTACAACAAGCAAAAGCCCAAATTAATGCAATGATTAATTCAAAAACA
    AATTATGATGTTGTATTCACTAGTGGTGCAACTGAATCCAATAATCTTGCTTTAAAAGGT
    ATTGCCTATCGTAAATTTGATACAGCGAAGGAAATAATTACATCCGTGTTAGAGCATCCG
    TCCGTATTAGAGGTTGTAAGATATTTGGAAGCACACGAAGGATTTAAAGTTAAATATGTT
    GATGTAAAGAAAGATGGCAGTATTAACTTAGAACACTTCAAAGAATTAGTGTCAGACAAA
    GTCGGTTTAGTAACATGTATGTATGTAAATAATGTAACTGGACAAATACAGCCTATTCCA
    CAAATGGCTAAAGTTATAAAAAATTATCCTAAGGCACATTTTCATGTAGATGCGGTTCAA
    GCATTCGGCAAAATTTCAATGGATCTCAATAACATAGATAGTATTAGTTTAAGTGGACAC
    AAGTTTAATGGTTTAAAAGGACAAGGCGTCTTACTTGTAAATCACATTCAAAATGTTGAA
    CCAACTGTCCATGGTGGTGGTCAAGAATATGGCGTTAGAAGTGGAACAGTTAATTTGCCA
    AATGATATTGCAATGGTTAAAGCGATGAAGATAGCTAATGAAAACTTTGAAGCATTGAAT
    GCATTTGTTACTGAGTTAAATAATGACGTCCGTCAATTTTTAAATAAATATCATGGAGTT
    TATATTAATTCTTCAACTTCAGGTTCACCATTCGTTTTAAATATTAGTTTTCCTGGCGTA
    AAAGGTGAAGTATTAGTTAATGCTTTTTCAAAATATGACATTATGATATCTACGACGAGT
    GCTTGTTCATCTAAACGTAATAAATTAAATGAAGTATTGGCTGCAATGGGATTATCAGAC
    AAATCTATTGAAGGTAGTATAAGATTATCATTTGGGGCTACTACAACTAAAGAAGATATA
    GCAAGGTTTAAAGAAATATTTATCATCATTTATGAGGAAATTAAGGAGTTGCTAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA1538 [new locus tag: SA_RS08655 ]
  • symbol: SA1538
  • description: hypothetical protein
  • length: 379
  • theoretical pI: 8.1916
  • theoretical MW: 41929.7
  • GRAVY: -0.143799

⊟Function[edit | edit source]

  • TIGRFAM:
    cysteine desulfurase NifS (TIGR03402; EC 2.8.1.7; HMM-score: 310.8)
    Genetic information processing DNA metabolism Restriction/modification cysteine desulfurase DndA (TIGR03235; EC 2.8.1.7; HMM-score: 298.8)
    Genetic information processing Protein synthesis tRNA and rRNA base modification cysteine desulfurase IscS (TIGR02006; EC 2.8.1.7; HMM-score: 281.3)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other cysteine desulfurase IscS (TIGR02006; EC 2.8.1.7; HMM-score: 281.3)
    cysteine desulfurase, NifS family (TIGR03403; EC 2.8.1.7; HMM-score: 250.3)
    and 8 more
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other cysteine desulfurase, SufS family (TIGR01979; HMM-score: 138)
    Unknown function Enzymes of unknown specificity cysteine desulfurase family protein (TIGR01977; HMM-score: 132.6)
    Unknown function Enzymes of unknown specificity cysteine desulfurase family protein (TIGR01976; HMM-score: 86)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other cysteine desulfurase, catalytic subunit CsdA (TIGR03392; EC 2.8.1.7; HMM-score: 75.9)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Glutathione and analogs ergothioneine biosynthesis PLP-dependent enzyme EgtE (TIGR04343; EC 4.4.-.-; HMM-score: 44.4)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other tyrosine decarboxylase MnfA (TIGR03812; EC 4.1.1.25; HMM-score: 29.3)
    Metabolism Central intermediary metabolism Phosphorus compounds 2-aminoethylphosphonate--pyruvate transaminase (TIGR02326; EC 2.6.1.37; HMM-score: 24.9)
    putative modified peptide (TIGR04509; HMM-score: 12.4)
  • TheSEED  :
    • Putative cysteine desulfurase, associated with tRNA 4-thiouridine synthase
    Cofactors, Vitamins, Prosthetic Groups, Pigments Cofactors, Vitamins, Prosthetic Groups, Pigments - no subcategory Thiamin biosynthesis  Putative cysteine desulfurase, associated with tRNA 4-thiouridine synthase
    and 1 more
    Sulfur Metabolism Organic sulfur assimilation At5g37530 (CsdL protein family)  Putative cysteine desulfurase, associated with tRNA 4-thiouridine synthase
  • PFAM:
    PLP_aminotran (CL0061) Aminotran_5; Aminotransferase class-V (PF00266; HMM-score: 235.6)
    and 3 more
    DegT_DnrJ_EryC1; DegT/DnrJ/EryC1/StrS aminotransferase family (PF01041; HMM-score: 22.4)
    Beta_elim_lyase; Beta-eliminating lyase (PF01212; HMM-score: 16)
    EF_hand (CL0220) EF-hand_1; EF hand (PF00036; HMM-score: 13.9)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: pyridoxal 5'-phosphate
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.67
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.15
    • Extracellular Score: 0.17
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.013085
    • TAT(Tat/SPI): 0.000795
    • LIPO(Sec/SPII): 0.000999
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MIYLDNAATTKAFEEVLDTYLKVNQSMYYNPNSPHKAGLQANQLLQQAKAQINAMINSKTNYDVVFTSGATESNNLALKGIAYRKFDTAKEIITSVLEHPSVLEVVRYLEAHEGFKVKYVDVKKDGSINLEHFKELVSDKVGLVTCMYVNNVTGQIQPIPQMAKVIKNYPKAHFHVDAVQAFGKISMDLNNIDSISLSGHKFNGLKGQGVLLVNHIQNVEPTVHGGGQEYGVRSGTVNLPNDIAMVKAMKIANENFEALNAFVTELNNDVRQFLNKYHGVYINSSTSGSPFVLNISFPGVKGEVLVNAFSKYDIMISTTSACSSKRNKLNEVLAAMGLSDKSIEGSIRLSFGATTTKEDIARFKEIFIIIYEEIKELLK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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