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FunGene: 08-OCT-2024
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus JSNZ
- locus tag: JSNZ_001701
- pan locus tag?: SAUPAN004368000
- symbol: JSNZ_001701
- pan gene symbol?: nifZ
- synonym:
- product: cysteine desulfurase family protein
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: JSNZ_001701
- symbol: JSNZ_001701
- product: cysteine desulfurase family protein
- replicon: chromosome
- strand: -
- coordinates: 1743087..1744235
- length: 1149
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Gene ID:
- RefSeq:
- BioCyc:
- MicrobesOnline:
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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1141GTGTTTATTTTGATATATCTAGATAATGCGGCAACGACGAAAGCATTTGAAGAAGTGTTA
GATACTTATTTAAAAGTAAATCAATCAATGTATTATAATCCGAATAGTCCGCATAAAGCT
GGTTTGCAGGCAAATCAATTACTACAACAAGCAAAAGCCCAAATTAATGCAATGATTAAT
TCAAAAACAAATTATGATGTTGTATTCACTAGTGGTGCAACTGAATCCAATAATCTTGCT
TTAAAAGGTATTGCCTATCGTAAATTTGATACAGCGAAGGAAATAATTACATCCGTGTTA
GAGCATCCGTCCGTATTAGAGGTTGTAAGATATTTGGAAGCACACGAAGGATTTAAAGTT
AAATATGTTGATGTAAAGAAAGATGGCAGTATTAACTTAGAACACTTCAAAGAATTAATG
TCAGACAAAGTCGGTTTAGTAACATGTATGTATGTAAATAATGTAACTGGACAAATACAG
CCTATTCCACAAATGGCTAAAGTTATAAAAAATTATCCTAAGGCACATTTTCATGTAGAT
GCGGTTCAAGCATTCGGCAAAATTTCAATGGATTTCAATAACGTAGATAGTATTAGTTTA
AGTGGACACAAGTTTAATGGTTTAAAAGGCCAAGGCGTCTTACTTGTAAATCACATTCAA
AATGTTGAACCAACTGTCCATGGTGGTGGTCAAGAATATGGTGTTAGAAGTGGAACAGTT
AATTTGCCAAATGATATTGCAATGGTTAAAGCGATGAAGATAGCTAATGAAAACTTTGAA
GCATTGAATGCATTTGTTACTGAGTTAAATAATGACGTCCGTCAATTTTTAAATAAATAT
CATGGAGTTTATATTAATTCTTCAACTTCAGGTTCACCATTCGTTTTAAATATTAGTTTT
CCTGGCGTAAAAGGTGAAGTATTAGTTAATGCTTTTTCAAAATATGACATTATGATATCT
ACGACGAGTGCTTGTTCATCTAAACGTAATAAATTAAATGAAGTATTGGCTGCAATGGGA
TTATCAGACAAATCTATTGAAGGTAGTATAAGATTATCATTTGGGGCTACTACAACTAAA
GAAGATATAGCAAGGTTTAAAGAAATATTTATCATCATTTATGAGGAAATTAAGGAGTTG
CTAAAATAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: JSNZ_001701
- symbol: JSNZ_001701
- description: cysteine desulfurase family protein
- length: 382
- theoretical pI: 8.1916
- theoretical MW: 42355.3
- GRAVY: -0.123037
⊟Function[edit | edit source]
- TIGRFAM: cysteine desulfurase NifS (TIGR03402; EC 2.8.1.7; HMM-score: 309)DNA metabolism Restriction/modification cysteine desulfurase DndA (TIGR03235; EC 2.8.1.7; HMM-score: 295.3)Protein synthesis tRNA and rRNA base modification cysteine desulfurase IscS (TIGR02006; EC 2.8.1.7; HMM-score: 281.6)Biosynthesis of cofactors, prosthetic groups, and carriers Other cysteine desulfurase IscS (TIGR02006; EC 2.8.1.7; HMM-score: 281.6)cysteine desulfurase, NifS family (TIGR03403; EC 2.8.1.7; HMM-score: 249.1)and 8 moreBiosynthesis of cofactors, prosthetic groups, and carriers Other cysteine desulfurase, SufS family (TIGR01979; HMM-score: 139.6)Unknown function Enzymes of unknown specificity cysteine desulfurase family protein (TIGR01977; HMM-score: 130.3)Unknown function Enzymes of unknown specificity cysteine desulfurase family protein (TIGR01976; HMM-score: 84.3)Biosynthesis of cofactors, prosthetic groups, and carriers Other cysteine desulfurase, catalytic subunit CsdA (TIGR03392; EC 2.8.1.7; HMM-score: 74.7)Biosynthesis of cofactors, prosthetic groups, and carriers Glutathione and analogs ergothioneine biosynthesis PLP-dependent enzyme EgtE (TIGR04343; EC 4.4.-.-; HMM-score: 45.2)Biosynthesis of cofactors, prosthetic groups, and carriers Other tyrosine decarboxylase MnfA (TIGR03812; EC 4.1.1.25; HMM-score: 30.7)Central intermediary metabolism Phosphorus compounds 2-aminoethylphosphonate--pyruvate transaminase (TIGR02326; EC 2.6.1.37; HMM-score: 24.7)putative modified peptide (TIGR04509; HMM-score: 12.4)
- TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
- PFAM: PLP_aminotran (CL0061) Aminotran_5; Aminotransferase class-V (PF00266; HMM-score: 238.7)and 6 moreAminotran_1_2; Aminotransferase class I and II (PF00155; HMM-score: 29.9)DegT_DnrJ_EryC1; DegT/DnrJ/EryC1/StrS aminotransferase family (PF01041; HMM-score: 21.9)EF_hand (CL0220) EF-hand_1; EF hand domain (PF00036; HMM-score: 15.9)PLP_aminotran (CL0061) Beta_elim_lyase; Beta-eliminating lyase (PF01212; HMM-score: 15.3)no clan defined DUF7659; Family of unknown function (DUF7659) (PF24692; HMM-score: 11)DUF4473; Domain of unknown function (DUF4473) (PF14747; HMM-score: 8.2)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 9.67
- Cytoplasmic Membrane Score: 0.01
- Cellwall Score: 0.15
- Extracellular Score: 0.17
- Internal Helices: 0
- DeepLocPro: Cytoplasmic
- Cytoplasmic Score: 0.9108
- Cytoplasmic Membrane Score: 0.0004
- Cell wall & surface Score: 0.0006
- Extracellular Score: 0.0881
- LocateP:
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.010223
- TAT(Tat/SPI): 0.000871
- LIPO(Sec/SPII): 0.001494
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
- GI:
- RefSeq:
- UniProt:
⊟Protein sequence[edit | edit source]
- MFILIYLDNAATTKAFEEVLDTYLKVNQSMYYNPNSPHKAGLQANQLLQQAKAQINAMINSKTNYDVVFTSGATESNNLALKGIAYRKFDTAKEIITSVLEHPSVLEVVRYLEAHEGFKVKYVDVKKDGSINLEHFKELMSDKVGLVTCMYVNNVTGQIQPIPQMAKVIKNYPKAHFHVDAVQAFGKISMDFNNVDSISLSGHKFNGLKGQGVLLVNHIQNVEPTVHGGGQEYGVRSGTVNLPNDIAMVKAMKIANENFEALNAFVTELNNDVRQFLNKYHGVYINSSTSGSPFVLNISFPGVKGEVLVNAFSKYDIMISTTSACSSKRNKLNEVLAAMGLSDKSIEGSIRLSFGATTTKEDIARFKEIFIIIYEEIKELLK
⊟Experimental data[edit | edit source]
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
- Operon-mapper [1] : JSNZ_001699 < thiI < JSNZ_001701
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ Blanca Taboada, Karel Estrada, Ricardo Ciria, Enrique Merino
Operon-mapper: a web server for precise operon identification in bacterial and archaeal genomes.
Bioinformatics: 2018, 34(23);4118-4120
[PubMed:29931111] [WorldCat.org] [DOI] (I p)