PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_RS03825 [old locus tag: SAUSA300_0712 ]
• pan locus tag^?: SAUPAN002623000
• symbol: SAUSA300_RS03825
• pan gene symbol^?: dtpT
• synonym:
• product: MFS transporter

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_RS03825 [old locus tag: SAUSA300_0712 ]
• symbol: SAUSA300_RS03825
• product: MFS transporter
• replicon: chromosome
• strand: -
• coordinates: 789740..791245
• length: 1506
• essential: unknown other strains

⊟Accession numbers

• Location: NC_007793 (789740..791245) NCBI
• BioCyc: SAUSA300_RS03825 BioCyc
• MicrobesOnline: see SAUSA300_0712

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  1141
  1201
  1261
  1321
  1381
  1441
  1501
  
  ATGACACAACAAAACTCCCATGGAAATCAAATTCAAGACATACCTCAAACAGGATTTTTC
  GGGCATCCTCGAGGACTAGGCGTACTCTTCTTTGTAGAGTTCTGGGAAAGGTTTAGTTAT
  TATGGCATGCGTGCCCTACTCATTTTCTACATGTACTTTGCCGTAACAGATAATGGCCTT
  GGAATTGATAAAACAACAGCTATGTCAATTATGTCAGTTTATGGTTCATTAATCTATATG
  ACATCAATACCAGGCGGATGGATTGCTGACAGAATTACAGGCACTAGAGGCGCTACTTTA
  TTAGGTGCAGTCTTTATTATTATCGGACATATTTGTTTAAGCTTACCATTTGCATTAATC
  GGCTTATTCACATCAATGTTCTTCATCATTATTGGTTCAGGTTTAATGAAGCCAAACATT
  TCAAATATCGTTGGCCGTTTATATCCTGAAAATGATAGACGTATGGATGCAGGTTTTGTT
  ATTTTCTATATGTCAGTTAATATGGGTGCATTATTATCACCTATTATTTTGCAACACTTT
  GTTAATGTTAAAAACTTCCACGGCGGATTCTTGATTGCAGCAGTTGGTATGGCATTAGGT
  TTAGTATGGTATGTACTTTTCAACCGCAAAAACTTAGGTAGCGTTGGTATGAAACCTACT
  AACCCATTGACACCAGCTGAAAAGAAAAAGTATGGTCTTATTATCGGAAGTGTTGTTTTA
  GCAATTGTATTAATTATCGTTATTGGTGCATTAACTAACTCATTATCATTTAACTTAGTT
  AGTAATACTGTTTTAGTATTAGGTATTGCATTACCAATCATTTACTTCACTTTAATTATT
  AGAAGTAAAGATGTCACTGATACTGAACGTTCTCGTGTTAAAGCATTTATTCCATTATTT
  ATTCTTGGAATGGTGTTCTGGGCTATTCAAGAACAAGGGTCTAATGTATTAAACATATAT
  GGAATTGAACATTCAGATATGAAATTAAACTTATTTGGTTGGAAAACAAACTTTGGTGAA
  GCGATCTTCCAATCAATTAACCCATTATTTATTTTATTACTAGCTCCAATTATTTCACTT
  TTATGGCAAAAGCTTGGAACTAAACAACCTAGCCTGCCAGTAAAATTTGCAATTGGTACG
  TTCTTAGCAGGTGCGTCATACATACTAATTGGTATTGTAGGTTATGCATCAGGTTCATCA
  AACTTTTCAGTTAACTGGGTTATTTTATCGTACATTATTTGTGTTATCGGTGAGCTATGC
  TTATCACCAACGGGTAATAGTGCTGCTGTTAAGTTAGCACCTAAAGCATTTAATGCCCAA
  ATGATGAGTATTTGGTACTTAACTAACGCTTCTGCACAAGCAATCAATGGTACTTTAGTT
  AAGTTAATAGAACCACTAGGTCAAACAAATTACTTTATTTTCCTAGGTGTTGTTGCAATT
  ATTGTTACAACAATTGTATTAGCAATCTCACCTTTAATCATCAAAGCGATGAAAGGTATA
  CGTTAA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  780
  840
  900
  960
  1020
  1080
  1140
  1200
  1260
  1320
  1380
  1440
  1500
  1506
  

⊟Protein

⊟General

• locus tag: SAUSA300_RS03825 [old locus tag: SAUSA300_0712 ]
• symbol: SAUSA300_RS03825
• description: MFS transporter
• length: 501
• theoretical pI: 9.98893
• theoretical MW: 54793.8
• GRAVY: 0.731337

⊟Function

• TIGRFAM:
  Metabolism Transport and binding proteins Amino acids, peptides and amines amino acid/peptide transporter (Peptide:H+ symporter) (TIGR00924; HMM-score: 364.8)
  and 1 more
  Metabolism Transport and binding proteins Amino acids, peptides and amines oligopeptide transporter, peptide:H+ symporter (TIGR00926; HMM-score: 70.9)
• TheSEED: see SAUSA300_0712
• PFAM:
  MFS (CL0015) PTR2; POT family (PF00854; HMM-score: 210)
  and 2 more
  MFS_1; Major Facilitator Superfamily (PF07690; HMM-score: 70.4)
  no clan defined GP41; Retroviral envelope protein (PF00517; HMM-score: 8.9)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 14
• DeepLocPro: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 0.9997
  • Cell wall & surface Score: 0
  • Extracellular Score: 0.0003
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.001554
  • TAT(Tat/SPI): 0.000134
  • LIPO(Sec/SPII): 0.000376
• predicted transmembrane helices (TMHMM): 13

⊟Accession numbers

• GI: 446115897 NCBI
• RefSeq: WP_000193752 NCBI
• UniProt: see SAUSA300_0712

⊟Protein sequence

• MTQQNSHGNQIQDIPQTGFFGHPRGLGVLFFVEFWERFSYYGMRALLIFYMYFAVTDNGLGIDKTTAMSIMSVYGSLIYMTSIPGGWIADRITGTRGATLLGAVFIIIGHICLSLPFALIGLFTSMFFIIIGSGLMKPNISNIVGRLYPENDRRMDAGFVIFYMSVNMGALLSPIILQHFVNVKNFHGGFLIAAVGMALGLVWYVLFNRKNLGSVGMKPTNPLTPAEKKKYGLIIGSVVLAIVLIIVIGALTNSLSFNLVSNTVLVLGIALPIIYFTLIIRSKDVTDTERSRVKAFIPLFILGMVFWAIQEQGSNVLNIYGIEHSDMKLNLFGWKTNFGEAIFQSINPLFILLLAPIISLLWQKLGTKQPSLPVKFAIGTFLAGASYILIGIVGYASGSSNFSVNWVILSYIICVIGELCLSPTGNSAAVKLAPKAFNAQMMSIWYLTNASAQAINGTLVKLIEPLGQTNYFIFLGVVAIIVTTIVLAISPLIIKAMKGIR

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: CodY see SAUSA300_0712

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You can add further information about the gene and protein here. [edit]

⊟Literature

⊟References

⊟Relevant publications