PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_0712 [new locus tag: SAUSA300_RS03825 ]
• pan locus tag^?: SAUPAN002623000
• symbol: SAUSA300_0712
• pan gene symbol^?: dtpT
• synonym:
• product: amino acid/peptide transporter (peptide:H+ symporter)

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_0712 [new locus tag: SAUSA300_RS03825 ]
• symbol: SAUSA300_0712
• product: amino acid/peptide transporter (peptide:H+ symporter)
• replicon: chromosome
• strand: -
• coordinates: 789740..791245
• length: 1506
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3914236 NCBI
• RefSeq: YP_493415 NCBI
• BioCyc: see SAUSA300_RS03825
• MicrobesOnline: 1292227 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

• 1
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  1501
  
  ATGACACAACAAAACTCCCATGGAAATCAAATTCAAGACATACCTCAAACAGGATTTTTC
  GGGCATCCTCGAGGACTAGGCGTACTCTTCTTTGTAGAGTTCTGGGAAAGGTTTAGTTAT
  TATGGCATGCGTGCCCTACTCATTTTCTACATGTACTTTGCCGTAACAGATAATGGCCTT
  GGAATTGATAAAACAACAGCTATGTCAATTATGTCAGTTTATGGTTCATTAATCTATATG
  ACATCAATACCAGGCGGATGGATTGCTGACAGAATTACAGGCACTAGAGGCGCTACTTTA
  TTAGGTGCAGTCTTTATTATTATCGGACATATTTGTTTAAGCTTACCATTTGCATTAATC
  GGCTTATTCACATCAATGTTCTTCATCATTATTGGTTCAGGTTTAATGAAGCCAAACATT
  TCAAATATCGTTGGCCGTTTATATCCTGAAAATGATAGACGTATGGATGCAGGTTTTGTT
  ATTTTCTATATGTCAGTTAATATGGGTGCATTATTATCACCTATTATTTTGCAACACTTT
  GTTAATGTTAAAAACTTCCACGGCGGATTCTTGATTGCAGCAGTTGGTATGGCATTAGGT
  TTAGTATGGTATGTACTTTTCAACCGCAAAAACTTAGGTAGCGTTGGTATGAAACCTACT
  AACCCATTGACACCAGCTGAAAAGAAAAAGTATGGTCTTATTATCGGAAGTGTTGTTTTA
  GCAATTGTATTAATTATCGTTATTGGTGCATTAACTAACTCATTATCATTTAACTTAGTT
  AGTAATACTGTTTTAGTATTAGGTATTGCATTACCAATCATTTACTTCACTTTAATTATT
  AGAAGTAAAGATGTCACTGATACTGAACGTTCTCGTGTTAAAGCATTTATTCCATTATTT
  ATTCTTGGAATGGTGTTCTGGGCTATTCAAGAACAAGGGTCTAATGTATTAAACATATAT
  GGAATTGAACATTCAGATATGAAATTAAACTTATTTGGTTGGAAAACAAACTTTGGTGAA
  GCGATCTTCCAATCAATTAACCCATTATTTATTTTATTACTAGCTCCAATTATTTCACTT
  TTATGGCAAAAGCTTGGAACTAAACAACCTAGCCTGCCAGTAAAATTTGCAATTGGTACG
  TTCTTAGCAGGTGCGTCATACATACTAATTGGTATTGTAGGTTATGCATCAGGTTCATCA
  AACTTTTCAGTTAACTGGGTTATTTTATCGTACATTATTTGTGTTATCGGTGAGCTATGC
  TTATCACCAACGGGTAATAGTGCTGCTGTTAAGTTAGCACCTAAAGCATTTAATGCCCAA
  ATGATGAGTATTTGGTACTTAACTAACGCTTCTGCACAAGCAATCAATGGTACTTTAGTT
  AAGTTAATAGAACCACTAGGTCAAACAAATTACTTTATTTTCCTAGGTGTTGTTGCAATT
  ATTGTTACAACAATTGTATTAGCAATCTCACCTTTAATCATCAAAGCGATGAAAGGTATA
  CGTTAA
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  1506
  

⊟Protein

⊟General

• locus tag: SAUSA300_0712 [new locus tag: SAUSA300_RS03825 ]
• symbol: SAUSA300_0712
• description: amino acid/peptide transporter (peptide:H+ symporter)
• length: 501
• theoretical pI: 9.98893
• theoretical MW: 54793.8
• GRAVY: 0.731337

⊟Function

• TIGRFAM:
  Metabolism Transport and binding proteins Amino acids, peptides and amines amino acid/peptide transporter (Peptide:H+ symporter) (TIGR00924; HMM-score: 364.8)
  and 1 more
  Metabolism Transport and binding proteins Amino acids, peptides and amines oligopeptide transporter, peptide:H+ symporter (TIGR00926; HMM-score: 70.9)
• TheSEED  :
  • Di/tripeptide permease YjdL
  Membrane Transport Uni- Sym- and Antiporters Proton-dependent Peptide Transporters  Di/tripeptide permease YjdL
• PFAM:
  MFS (CL0015) PTR2; POT family (PF00854; HMM-score: 202.6)
  and 1 more
  MFS_1; Major Facilitator Superfamily (PF07690; HMM-score: 63.7)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 14
• LocateP: Multi-transmembrane
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.001554
  • TAT(Tat/SPI): 0.000134
  • LIPO(Sec/SPII): 0.000376
• predicted transmembrane helices (TMHMM): 13

⊟Accession numbers

• GI: 87161494 NCBI
• RefSeq: YP_493415 NCBI
• UniProt: A0A0H2XIN4 UniProt

⊟Protein sequence

• MTQQNSHGNQIQDIPQTGFFGHPRGLGVLFFVEFWERFSYYGMRALLIFYMYFAVTDNGLGIDKTTAMSIMSVYGSLIYMTSIPGGWIADRITGTRGATLLGAVFIIIGHICLSLPFALIGLFTSMFFIIIGSGLMKPNISNIVGRLYPENDRRMDAGFVIFYMSVNMGALLSPIILQHFVNVKNFHGGFLIAAVGMALGLVWYVLFNRKNLGSVGMKPTNPLTPAEKKKYGLIIGSVVLAIVLIIVIGALTNSLSFNLVSNTVLVLGIALPIIYFTLIIRSKDVTDTERSRVKAFIPLFILGMVFWAIQEQGSNVLNIYGIEHSDMKLNLFGWKTNFGEAIFQSINPLFILLLAPIISLLWQKLGTKQPSLPVKFAIGTFLAGASYILIGIVGYASGSSNFSVNWVILSYIICVIGELCLSPTGNSAAVKLAPKAFNAQMMSIWYLTNASAQAINGTLVKLIEPLGQTNYFIFLGVVAIIVTTIVLAISPLIIKAMKGIR

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator: CodY (repression) regulon

  CodY

  (TF)

  important in Amino acid metabolism; RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications