PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_RS01690 [old locus tag: SAUSA300_0317 ]
• pan locus tag^?: SAUPAN001241000
• symbol: SAUSA300_RS01690
• pan gene symbol^?: nanR
• synonym:
• product: MurR/RpiR family transcriptional regulator

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_RS01690 [old locus tag: SAUSA300_0317 ]
• symbol: SAUSA300_RS01690
• product: MurR/RpiR family transcriptional regulator
• replicon: chromosome
• strand: -
• coordinates: 368949..369749
• length: 801
• essential: unknown other strains

⊟Accession numbers

• Location: NC_007793 (368949..369749) NCBI
• BioCyc: SAUSA300_RS01690 BioCyc
• MicrobesOnline: see SAUSA300_0317

⊟Phenotype

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⊟DNA sequence

• 1
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  ATGAAATTTGAGAATCGTGTTCAACGTTATCAACATTTATTTACAAAAACTGATAAACGC
  ATCGTCAATTATATAAGACAAAATGGTTATAGCGATGCCTTTTCAACGATTAACTCCTTA
  GCCCATGCGATTGGTACATCACCAGCAACGATGACACGCTTTAGTCATAAGCTGGATTAT
  GAGAATTTCCAAGATTTAAAATTTAATATTCAACAAGAAATGACAGAGACAGTTATTGAA
  AATAGCCCAATTATTCAAAGAATTCATAAATATCATCAACAAATCATTCAACAAACTGGT
  GAATTTATTGATAACGACATTATCCAAACCTTTATCGACAAACTTCAATCAAGTCGACAT
  ATACTCTTTGCTGGACTAGGTAGCTCTGGTTTATCAGCGACTGAATTTTATTATCGTATG
  ATTCGTATGGGGCTAAAAGGTAATGTTACTACCGATTCACATTTAATGAAAATATCGGCA
  TCCCTACTATCTCATTCGGATATGTTTATTGCTATGTCAAATAGTGGTAATACTTCGGAA
  TTAATTTCTGCAGCGGAAGTCGCCAAATCTCATGGTGCATATGTCGTTGCCATCACAAAT
  TTTGAAGGTAGTAAACTTACAGATTGTGCAGATTTAGTACTTTTAACAACGGATCAATCG
  CGTAATACCGACCATCAATTTATCAACACACAAATTGCGACACTCTTTTTAATCGATATC
  GTGAGTTATCATTTATTAGAAAATACGAATCTGAGTCAAACTTATCAACATACTAAATCT
  ATTATCCTAGACAACAAATAA
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⊟Protein

⊟General

• locus tag: SAUSA300_RS01690 [old locus tag: SAUSA300_0317 ]
• symbol: SAUSA300_RS01690
• description: MurR/RpiR family transcriptional regulator
• length: 266
• theoretical pI: 6.97071
• theoretical MW: 30258
• GRAVY: -0.282707

⊟Function

• TIGRFAM:
  6-phospho 3-hexuloisomerase (TIGR03127; EC 5.3.-.-; HMM-score: 73.6)
  and 7 more
  Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan glutamine-fructose-6-phosphate transaminase (isomerizing) (TIGR01135; EC 2.6.1.16; HMM-score: 38.1)
  Metabolism Central intermediary metabolism Amino sugars glutamine-fructose-6-phosphate transaminase (isomerizing) (TIGR01135; EC 2.6.1.16; HMM-score: 38.1)
  bifunctional phosphoglucose/phosphomannose isomerase (TIGR02128; EC 5.3.1.9; HMM-score: 33.7)
  Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides phosphoheptose isomerase (TIGR00441; EC 5.3.1.28; HMM-score: 27.7)
  Metabolism Energy metabolism Sugars sugar isomerase, KpsF/GutQ family (TIGR00393; HMM-score: 23.9)
  Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan N-acetylmuramic acid 6-phosphate etherase (TIGR00274; EC 4.2.1.126; HMM-score: 21.1)
  Genetic information processing DNA metabolism DNA replication, recombination, and repair DNA topoisomerase IV, B subunit (TIGR01058; EC 5.99.1.-; HMM-score: 14.3)
• TheSEED: see SAUSA300_0317
• PFAM:
  SIS (CL0067) SIS; SIS domain (PF01380; HMM-score: 57.8)
  HTH (CL0123) HTH_6; Helix-turn-helix domain, rpiR family (PF01418; HMM-score: 50.5)
  and 6 more
  SIS (CL0067) SIS_2; SIS domain (PF13580; HMM-score: 42.8)
  HTH (CL0123) Dimerisation2; Dimerisation domain (PF16864; HMM-score: 19)
  HTH_24; Winged helix-turn-helix DNA-binding (PF13412; HMM-score: 15.3)
  no clan defined TyrRSs_C; Tyrosyl-tRNA synthetase C-terminal domain (PF16714; HMM-score: 14.1)
  DUF1643; Protein of unknown function (DUF1643) (PF07799; HMM-score: 13)
  HTH (CL0123) MarR; MarR family (PF01047; HMM-score: 12.6)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:
• genes regulated by NanR*, TF important in Sialic acid utilization, N-acetylglucosamine utilization: see SAUSA300_0317

⊟Localization

• PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 2.5
  • Cytoplasmic Membrane Score: 2.5
  • Cellwall Score: 2.5
  • Extracellular Score: 2.5
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.023801
  • TAT(Tat/SPI): 0.008751
  • LIPO(Sec/SPII): 0.003808
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446590036 NCBI
• RefSeq: WP_000667382 NCBI
• UniProt: see SAUSA300_0317

⊟Protein sequence

• MKFENRVQRYQHLFTKTDKRIVNYIRQNGYSDAFSTINSLAHAIGTSPATMTRFSHKLDYENFQDLKFNIQQEMTETVIENSPIIQRIHKYHQQIIQQTGEFIDNDIIQTFIDKLQSSRHILFAGLGSSGLSATEFYYRMIRMGLKGNVTTDSHLMKISASLLSHSDMFIAMSNSGNTSELISAAEVAKSHGAYVVAITNFEGSKLTDCADLVLLTTDQSRNTDHQFINTQIATLFLIDIVSYHLLENTNLSQTYQHTKSIILDNK

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: NanR* see SAUSA300_0317

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications