PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_2224 [new locus tag: SAUSA300_RS12285 ]
• pan locus tag^?: SAUPAN005734000
• symbol: moeA
• pan gene symbol^?: moeA
• synonym:
• product: molybdopterin biosynthesis protein A

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_2224 [new locus tag: SAUSA300_RS12285 ]
• symbol: moeA
• product: molybdopterin biosynthesis protein A
• replicon: chromosome
• strand: -
• coordinates: 2392087..2393346
• length: 1260
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3913407 NCBI
• RefSeq: YP_494859 NCBI
• BioCyc: see SAUSA300_RS12285
• MicrobesOnline: 1293739 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

• 1
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  ATGGTAGTAGAAAAAAGAAACCCAATCCCAGTTAAAGAAGCAATTCAACGTATCGTTAAT
  CAGCAGAGTTCAATGCCGGCAATTACGGTAGCACTTGAAAAAAGTCTAAATCATATCTTA
  GCAGAAGATATTGTAGCTACTTATGATATACCAAGGTTTGATAAATCACCTTATGATGGT
  TTTGCAATTCGCAGTGTTGATTCACAAGGGGCAAGTGGTCAGAATCGCATTGAGTTTAAA
  GTGATTGATCATATTGGTGCAGGTTCAGTTTCTGATAAATTAGTTGGGGATCACGAAGCG
  GTGCGTATTATGACTGGAGCACAAATACCTAATGGCGCAGATGCTGTTGTTATGTTTGAA
  CAAACGATTGAACTAGAAGATACATTTACAATTCGTAAACCATTTTCAAAAAATGAAAAT
  ATATCTTTAAAAGGTGAAGAAACAAAGACAGGCGATGTTGTTCTAAAAAAAGGACAAGTA
  ATTAATCCAGGGGCTATCGCGGTCCTTGCAACATATGGCTATGCAGAGGTTAAAGTTATT
  AAGCAACCGAGTGTCGCTGTTATTGCAACAGGAAGCGAATTATTAGATGTTAATGATGTA
  TTAGAAGATGGAAAAATTCGTAACTCTAATGGCCCAATGATTCGTGCCTTAGCAGAAAAA
  TTAGGTCTTGAAGTTGGTATTTACAAAACACAAAAAGATGATTTAGATAGTGGCATCCAA
  GTCGTTAAAGAAGCTATGGAAAAACATGATATCGTTATTACAACGGGCGGAGTTTCTGTT
  GGAGATTTTGACTATTTACCTGAGATTTATAAGGCTGTAAAGGCGGAAGTGTTATTTAAT
  AAAGTAGCAATGCGTCCTGGTAGCGTAACAACGGTTGCATTTGTAGATGGAAAGTATTTG
  TTTGGATTATCTGGAAATCCATCAGCTTGTTTTACAGGATTTGAACTATTTGTGAAACCA
  GCTGTTAAACATATGTGTGGCGCACTAGAAGTCTTCCCGCAAATAATTAAAGCAACATTA
  ATGGAAGATTTTACCAAGGCAAACCCATTCACACGATTTATACGTGCTAAAGCAACGTTA
  ACAAGTGCTGGAGCTACTGTAGTACCTTCAGGATTCAATAAATCAGGTGCGGTTGTAGCG
  ATTGCACATGCTAACTGTATGGTCATGTTACCAGGAGGGTCACGTGGTTTTAAAGCGGGG
  CATACAGTAGATATTATATTGACTGAATCTGACGCTGCTGAAGAGGAACTTCTTTTATGA
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⊟Protein

⊟General

• locus tag: SAUSA300_2224 [new locus tag: SAUSA300_RS12285 ]
• symbol: MoeA
• description: molybdopterin biosynthesis protein A
• length: 419
• theoretical pI: 5.50634
• theoretical MW: 45016.5
• GRAVY: 0.0575179

⊟Function

• TIGRFAM:
  molybdenum cofactor synthesis domain (TIGR00177; HMM-score: 113.2)
  and 1 more
  Genetic information processing DNA metabolism DNA replication, recombination, and repair competence/damage-inducible protein CinA N-terminal domain (TIGR00200; HMM-score: 23.5)
• TheSEED  :
  • Molybdopterin biosynthesis protein MoeA
  Cofactors, Vitamins, Prosthetic Groups, Pigments Folate and pterines Molybdenum cofactor biosynthesis  Molybdopterin biosynthesis protein MoeA
• PFAM:
  no clan defined MoeA_N; MoeA N-terminal region (domain I and II) (PF03453; HMM-score: 139.4)
  and 2 more
  MoCF_biosynth; Probable molybdopterin binding domain (PF00994; HMM-score: 94)
  MoeA_C; MoeA C-terminal region (domain IV) (PF03454; HMM-score: 59.6)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.009777
  • TAT(Tat/SPI): 0.00108
  • LIPO(Sec/SPII): 0.00068
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87160744 NCBI
• RefSeq: YP_494859 NCBI
• UniProt: A0A0H2XF86 UniProt

⊟Protein sequence

• MVVEKRNPIPVKEAIQRIVNQQSSMPAITVALEKSLNHILAEDIVATYDIPRFDKSPYDGFAIRSVDSQGASGQNRIEFKVIDHIGAGSVSDKLVGDHEAVRIMTGAQIPNGADAVVMFEQTIELEDTFTIRKPFSKNENISLKGEETKTGDVVLKKGQVINPGAIAVLATYGYAEVKVIKQPSVAVIATGSELLDVNDVLEDGKIRNSNGPMIRALAEKLGLEVGIYKTQKDDLDSGIQVVKEAMEKHDIVITTGGVSVGDFDYLPEIYKAVKAEVLFNKVAMRPGSVTTVAFVDGKYLFGLSGNPSACFTGFELFVKPAVKHMCGALEVFPQIIKATLMEDFTKANPFTRFIRAKATLTSAGATVVPSGFNKSGAVVAIAHANCMVMLPGGSRGFKAGHTVDIILTESDAAEEELLL

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: moaA < mobA < moaD < moaE < mobB < moeA

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications