PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_2223 [new locus tag: SAUSA300_RS12280 ]
• pan locus tag^?: SAUPAN005733000
• symbol: mobB
• pan gene symbol^?: mobB
• synonym:
• product: molybdopterin-guanine dinucleotide biosynthesis protein B

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_2223 [new locus tag: SAUSA300_RS12280 ]
• symbol: mobB
• product: molybdopterin-guanine dinucleotide biosynthesis protein B
• replicon: chromosome
• strand: -
• coordinates: 2391605..2392090
• length: 486
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3913253 NCBI
• RefSeq: YP_494858 NCBI
• BioCyc: see SAUSA300_RS12280
• MicrobesOnline: 1293738 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGATTTTACAAATTGTAGGTTACAAAAAGTCTGGTAAGACAACATTGATGAGGCATATT
  GTCTCTTTCTTAAAGTCACATGGTTATACAGTTGCTACTATTAAACATCATGGGCATGGT
  AAGGAAGATATTCAATTACAGGATTCAGACGTCGATCACATGAAGCATTTTGAAGCGGGG
  GCAGATCAAAGTATTGTACAAGGTTTTCAATATCAGCAAACTGTAACACGTGTAGATAAT
  CAAAATCTTACTCAAATTATTGAAAAATCTGTTACAATTGACACCAATATCGTATTAGTT
  GAAGGCTTTAAAAATGCTGATTTTGAAAAAGTCGTAGTCTATCGAAATGAAGAAGAGTTG
  CAAGTATTACAACAATTGTCGAATGTTTGTTATAGCATTAATGTAAGGGAGCATGAAGAT
  TTTACAGCATTTGAGCAATGGTTATTAAATAAAATTAAAAATGATTGTGATACACAATTA
  ACATAG
  60
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  486
  

⊟Protein

⊟General

• locus tag: SAUSA300_2223 [new locus tag: SAUSA300_RS12280 ]
• symbol: MobB
• description: molybdopterin-guanine dinucleotide biosynthesis protein B
• length: 161
• theoretical pI: 6.07381
• theoretical MW: 18568.8
• GRAVY: -0.43913

⊟Function

• TIGRFAM:
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Molybdopterin molybdopterin-guanine dinucleotide biosynthesis protein B (TIGR00176; HMM-score: 133.4)
  and 9 more
  Signal transduction Regulatory functions DNA interactions heavy metal response regulator (TIGR01387; HMM-score: 16.5)
  2-aminoethylphosphonate ABC transport system, ATP-binding component PhnT (TIGR03258; HMM-score: 14.4)
  Genetic information processing Protein fate Protein and peptide secretion and trafficking heme ABC exporter, ATP-binding protein CcmA (TIGR01189; EC 3.6.3.41; HMM-score: 13.6)
  Metabolism Transport and binding proteins Other heme ABC exporter, ATP-binding protein CcmA (TIGR01189; EC 3.6.3.41; HMM-score: 13.6)
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Salvage of nucleosides and nucleotides uridine kinase (TIGR00235; EC 2.7.1.48; HMM-score: 12.9)
  Metabolism Amino acid biosynthesis Aromatic amino acid family chorismate mutase (TIGR01807; EC 5.4.99.5; HMM-score: 12.4)
  Unknown function General small GTP-binding protein domain (TIGR00231; HMM-score: 12.1)
  Metabolism Transport and binding proteins Unknown substrate anchored repeat-type ABC transporter, ATP-binding subunit (TIGR03771; HMM-score: 11.8)
  Cellular processes Cellular processes Sporulation and germination stage V sporulation protein B (TIGR02900; HMM-score: 11.4)
• TheSEED  :
  • Molybdopterin-guanine dinucleotide biosynthesis protein MobB
  Cofactors, Vitamins, Prosthetic Groups, Pigments Folate and pterines Molybdenum cofactor biosynthesis  Molybdopterin-guanine dinucleotide biosynthesis protein MobB
• PFAM:
  P-loop_NTPase (CL0023) MobB; Molybdopterin guanine dinucleotide synthesis protein B (PF03205; HMM-score: 98.7)
  and 13 more
  ATPase_2; ATPase domain predominantly from Archaea (PF01637; HMM-score: 20.5)
  cobW; CobW/HypB/UreG, nucleotide-binding domain (PF02492; HMM-score: 17.9)
  AB_hydrolase (CL0028) Hydrolase_4; Serine aminopeptidase, S33 (PF12146; HMM-score: 15.6)
  P-loop_NTPase (CL0023) AAA_26; AAA domain (PF13500; HMM-score: 15.5)
  AAA_30; AAA domain (PF13604; HMM-score: 14.6)
  AAA_14; AAA domain (PF13173; HMM-score: 14)
  NTPase_1; NTPase (PF03266; HMM-score: 13.9)
  AAA_18; AAA domain (PF13238; HMM-score: 13.4)
  ABC_tran; ABC transporter (PF00005; HMM-score: 12.9)
  DUF2478; Protein of unknown function (DUF2478) (PF10649; HMM-score: 12.9)
  AAA_22; AAA domain (PF13401; HMM-score: 12.7)
  AAA_16; AAA ATPase domain (PF13191; HMM-score: 12.3)
  CbiA; CobQ/CobB/MinD/ParA nucleotide binding domain (PF01656; HMM-score: 12.1)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.014661
  • TAT(Tat/SPI): 0.000551
  • LIPO(Sec/SPII): 0.00264
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87160185 NCBI
• RefSeq: YP_494858 NCBI
• UniProt: A0A0H2XE14 UniProt

⊟Protein sequence

• MILQIVGYKKSGKTTLMRHIVSFLKSHGYTVATIKHHGHGKEDIQLQDSDVDHMKHFEAGADQSIVQGFQYQQTVTRVDNQNLTQIIEKSVTIDTNIVLVEGFKNADFEKVVVYRNEEELQVLQQLSNVCYSINVREHEDFTAFEQWLLNKIKNDCDTQLT

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: moaA < mobA < moaD < moaE < mobB < moeA

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications