PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_2220 [new locus tag: SAUSA300_RS12265 ]
• pan locus tag^?: SAUPAN005730000
• symbol: mobA
• pan gene symbol^?: mobA
• synonym:
• product: molybdopterin-guanine dinucleotide biosynthesis protein MobA

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_2220 [new locus tag: SAUSA300_RS12265 ]
• symbol: mobA
• product: molybdopterin-guanine dinucleotide biosynthesis protein MobA
• replicon: chromosome
• strand: -
• coordinates: 2390300..2390899
• length: 600
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3914564 NCBI
• RefSeq: YP_494855 NCBI
• BioCyc: see SAUSA300_RS12265
• MicrobesOnline: 1293735 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

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  ATGAAAGCAATAATTCTTGCAGGTGGTCATTCAGTGCGATTTGGTAAGCCCAAAGCTTTT
  GCGGAAGTGAACGGTGAGACCTTTTATAGTAGAGTAATTAAGACATTAGAATCAACAAAT
  ATGTTCAATGAAATTATTATTAGTACAAATGCGCAATTGGCAACGCAATTTAAATATCCA
  AATGTTGTTATAGATGATGAGAATCATAATGATAAAGGTCCATTAGCAGGAATTTATACA
  ATCATGAAGCAACATCCTGAAGAAGAATTGTTTTTTGTCGTTTCTGTTGATACACCAATG
  ATTACTGGTAAAGCTGTAAGCACGTTGTATCAGTTTTTAGTTTCTCATCTTATTGAAAAT
  CATTTAGATGTCGCAGCTTTTAAAGAAGATGGACGTTTTATTCCAACAATTGCATTTTAT
  AGTCCGAATGCATTAGGCGCTATAACTAAAGCACTACATTCTGATAATTACAGTTTTAAA
  AATGTATATCATGAATTATCAACGGATTATTTGGATGTAAGGGATGTAGATGCGCCCTCA
  TATTGGTACAAAAATATAAATTATCAGCATGATTTGGACGCTTTAATTCAAAAATTGTAA
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⊟Protein

⊟General

• locus tag: SAUSA300_2220 [new locus tag: SAUSA300_RS12265 ]
• symbol: MobA
• description: molybdopterin-guanine dinucleotide biosynthesis protein MobA
• length: 199
• theoretical pI: 5.81455
• theoretical MW: 22541.4
• GRAVY: -0.164824

⊟Function

• reaction:
  EC 2.7.7.77?  ExPASy
  Molybdenum cofactor guanylyltransferase GTP + molybdenum cofactor = diphosphate + guanylyl molybdenum cofactor
• TIGRFAM:
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Molybdopterin molybdenum cofactor guanylyltransferase (TIGR02665; EC 2.7.7.77; HMM-score: 88.2)
  and 9 more
  molybdenum cofactor cytidylyltransferase (TIGR03310; EC 2.7.7.76; HMM-score: 32.4)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (TIGR00453; EC 2.7.7.60; HMM-score: 28.1)
  Hypothetical proteins Conserved TIGR00454 family protein (TIGR00454; HMM-score: 22.5)
  Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase (TIGR01173; EC 2.3.1.157,2.7.7.23; HMM-score: 19.3)
  Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase (TIGR01173; EC 2.3.1.157,2.7.7.23; HMM-score: 19.3)
  Metabolism Central intermediary metabolism Amino sugars UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase (TIGR01173; EC 2.3.1.157,2.7.7.23; HMM-score: 19.3)
  xanthine dehydrogenase accessory protein pucB (TIGR03202; HMM-score: 16.2)
  pseudaminic acid cytidylyltransferase (TIGR03584; EC 2.7.7.81; HMM-score: 12.4)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other 2-phospho-L-lactate guanylyltransferase (TIGR03552; EC 2.7.7.68; HMM-score: 12.1)
• TheSEED  :
  • Molybdenum cofactor guanylyltransferase (EC 2.7.7.77)
  Cofactors, Vitamins, Prosthetic Groups, Pigments Folate and pterines Molybdenum cofactor biosynthesis  Molybdopterin-guanine dinucleotide biosynthesis protein MobA
• PFAM:
  GT-A (CL0110) NTP_transf_3; MobA-like NTP transferase domain (PF12804; HMM-score: 91.5)
  and 4 more
  IspD; 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (PF01128; HMM-score: 16.6)
  CTP_transf_3; Cytidylyltransferase (PF02348; HMM-score: 14.9)
  Metallo-HOrase (CL0381) Lactamase_B_6; Metallo-beta-lactamase superfamily domain (PF16661; HMM-score: 14.6)
  CheY (CL0304) RcsC; RcsC Alpha-Beta-Loop (ABL) (PF09456; HMM-score: 14.3)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors: Mg²⁺
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.051389
  • TAT(Tat/SPI): 0.000857
  • LIPO(Sec/SPII): 0.002827
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87161876 NCBI
• RefSeq: YP_494855 NCBI
• UniProt: Q2FEM3 UniProt

⊟Protein sequence

• MKAIILAGGHSVRFGKPKAFAEVNGETFYSRVIKTLESTNMFNEIIISTNAQLATQFKYPNVVIDDENHNDKGPLAGIYTIMKQHPEEELFFVVSVDTPMITGKAVSTLYQFLVSHLIENHLDVAAFKEDGRFIPTIAFYSPNALGAITKALHSDNYSFKNVYHELSTDYLDVRDVDAPSYWYKNINYQHDLDALIQKL

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: moaA < mobA < moaD < moaE < mobB < moeA

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

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⊟Literature

⊟References

⊟Relevant publications