PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_2062 [new locus tag: SAUSA300_RS11355 ]
• pan locus tag^?: SAUPAN005398000
• symbol: atpF
• pan gene symbol^?: atpF
• synonym:
• product: F0F1 ATP synthase subunit B

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_2062 [new locus tag: SAUSA300_RS11355 ]
• symbol: atpF
• product: F0F1 ATP synthase subunit B
• replicon: chromosome
• strand: -
• coordinates: 2225341..2225862
• length: 522
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3913609 NCBI
• RefSeq: YP_494708 NCBI
• BioCyc: see SAUSA300_RS11355
• MicrobesOnline: 1293577 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

• 1
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  GTGACTGAAACAGCTAACTTATTCGTTCTTGGTGCAGCTGGAGGCGTTGAGTGGGGTACT
  GTGATTGTACAGGTCCTAACTTTCATCGTGTTACTTGCGTTACTTAAAAAGTTCGCATGG
  GGTCCATTGAAAGATGTAATGGATAAACGTGAAAGAGATATTAACAGAGATATCGATGAC
  GCAGAACAAGCTAAGTTAAATGCACAGAAACTTGAAGAAGAAAATAAACAAAAACTTAAA
  GAAACACAAGAAGAAGTTCAAAAGATTTTAGAAGATGCTAAGGTTCAAGCACGTCAACAG
  CAAGAACAAATTATTCATGAAGCAAACGTACGTGCAAACGGTATGATTGAAACAGCACAA
  AGTGAAATCAATAGCCAAAAAGAACGTGCCATTGCAGATATTAATAATCAAGTATCTGAA
  CTATCAGTGTTAATTGCTTCTAAAGTTCTTAGAAAAGAAATTTCTGAACAAGACCAAAAA
  GCATTGGTTGACAAGTATCTAAAAGAGGCAGGCGATAAATAA
  60
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  522
  

⊟Protein

⊟General

• locus tag: SAUSA300_2062 [new locus tag: SAUSA300_RS11355 ]
• symbol: AtpF
• description: F0F1 ATP synthase subunit B
• length: 173
• theoretical pI: 4.75697
• theoretical MW: 19539.1
• GRAVY: -0.514451

⊟Function

• reaction:
  EC 3.6.3.14?  ExPASy
  H⁺-transporting two-sector ATPase ATP + H₂O + H⁺(In) = ADP + phosphate + H⁺(Out)
• TIGRFAM:
  Metabolism Energy metabolism ATP-proton motive force interconversion ATP synthase F0, B subunit (TIGR01144; EC 3.6.3.14; HMM-score: 124.4)
  and 7 more
  alternate F1F0 ATPase, F0 subunit B (TIGR03321; EC 3.6.3.-; HMM-score: 76)
  hydroxylamine oxidation protein HaoB (TIGR04392; HMM-score: 13.5)
  ATP synthase archaeal, H subunit (TIGR02926; EC 3.6.3.14; HMM-score: 12.4)
  Metabolism Transport and binding proteins Cations and iron carrying compounds ferrous iron transport protein B (TIGR00437; HMM-score: 7.3)
  two transmembrane protein (TIGR04527; HMM-score: 7)
  alternate F1F0 ATPase, F1 subunit alpha (TIGR03324; EC 3.6.3.-; HMM-score: 6.2)
  Metabolism Transport and binding proteins Cations and iron carrying compounds sodium/hydrogen antiporter (TIGR00844; HMM-score: 4.8)
• TheSEED  :
  • ATP synthase F0 sector subunit b (EC 7.1.2.2)
  Respiration ATP synthases F0F1-type ATP synthase  ATP synthase F0 sector subunit b
• PFAM:
  ATP_synthase (CL0255) ATP-synt_B; ATP synthase B/B' CF(0) (PF00430; HMM-score: 112.3)
  and 9 more
  Mt_ATP-synt_B; Mitochondrial ATP synthase B chain precursor (ATP-synt_B) (PF05405; HMM-score: 22.1)
  no clan defined LMBR1; LMBR1-like membrane protein (PF04791; HMM-score: 16.8)
  DivIVA; DivIVA protein (PF05103; HMM-score: 14.3)
  Monooxygenase_B; Monooxygenase subunit B protein (PF04744; HMM-score: 11.3)
  HBS1_N; HBS1 N-terminus (PF08938; HMM-score: 11.1)
  RapA_C; RNA polymerase recycling family C-terminal (PF12137; HMM-score: 10.8)
  YfcL; YfcL protein (PF08891; HMM-score: 9.8)
  Fib_alpha; Fibrinogen alpha/beta chain family (PF08702; HMM-score: 7.1)
  CPA_AT (CL0064) Mem_trans; Membrane transport protein (PF03547; HMM-score: 5.4)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 1.05
  • Cytoplasmic Membrane Score: 8.78
  • Cellwall Score: 0.08
  • Extracellular Score: 0.09
  • Internal Helix: 1
• LocateP: N-terminally anchored (No CS)
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 4
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.005976
  • TAT(Tat/SPI): 0.00052
  • LIPO(Sec/SPII): 0.002479
• predicted transmembrane helices (TMHMM): 1

⊟Accession numbers

• GI: 87162300 NCBI
• RefSeq: YP_494708 NCBI
• UniProt: Q2FF20 UniProt

⊟Protein sequence

• MTETANLFVLGAAGGVEWGTVIVQVLTFIVLLALLKKFAWGPLKDVMDKRERDINRDIDDAEQAKLNAQKLEEENKQKLKETQEEVQKILEDAKVQARQQQEQIIHEANVRANGMIETAQSEINSQKERAIADINNQVSELSVLIASKVLRKEISEQDQKALVDKYLKEAGDK

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: atpC < atpD < atpG < atpA < atpH < atpF

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications