From AureoWiki
Jump to navigation Jump to search

NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_1700 [new locus tag: SAUSA300_RS09285 ]
  • pan locus tag?: SAUPAN004432000
  • symbol: SAUSA300_1700
  • pan gene symbol?: —
  • synonym:
  • product: polysaccharide biosynthesis protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_1700 [new locus tag: SAUSA300_RS09285 ]
  • symbol: SAUSA300_1700
  • product: polysaccharide biosynthesis protein
  • replicon: chromosome
  • strand: -
  • coordinates: 1874455..1876116
  • length: 1662
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGAGTGAAAGTAAAGAAATGGTGCGTGGAACCTTTTTAATTACCATTAGTATATTAATT
    ACCAAGGTATTGGGCGTACTTTTTATCATTCCATTCAACTATTTAATTGGTGGACAAGAA
    AATATGGCGCCGTTCACATACGCTTATGCACCATATAATATTGCAATTGCAGTTGCGACA
    GCAGGTGTACCATTAGCAGCTTCGAAATATGTTGCGAAATATAATGCTATTGGGGCATAT
    AAAGTAAGTCAGAAATTTTATAAATCGAGTTTTATTGTAATGAGTATTACCGGTGTTTTA
    GGTTTCTTGGTACTGTATTTCTTAGCACCTTATATTTCTGAATTAACATTAGCTAGAAAC
    ATTCATGATAAAAACGGTTGGTCAGTCGATGATATTACTTGGATTATAAGAATCATTAGT
    ATGGTTGTTATCTTTATTCCTGTATTAGCGACATGGAGAGGGATATTCCAAGGTTATAAA
    TCTATGGGGCCAACAGCAGTGTCAGAAGTAACTGAGCAAATTGCACGCGTGATTTTCATA
    TTAATCGGAAGTTATTTAGTACTAAATGTCTTTGATGGCTCTATTTTATTAGCCAATGGT
    ATTGCTACTTTTGCAGCAGCAGTTGGTGCGATTATTGGTATATTCACACTCTGGTATTAC
    TGGAGAAAGCGTAAGCATAATATTGATCGTATGGTCGAATCTGATTACACAGATATAGAT
    GTGTCATATGGCAAGATGTATAAAGAAATTATTGCTTATAGTATACCGTTCGTTATTGTA
    AGTTTGAATTATCCATTATTTAACTTAGTAGATCAATTTACACATAACGGTGCTTTATCT
    TTAGTTGGTATACCTTCACAGTTACAAGATATATTCTTTAATATGCTAAATATGTCAACT
    AATAAAATTGTAATGATTCCGACATCATTAAGTGCTGGTTTTGCAGTGAGTTTAATTCCT
    TATATTACAAAAACATTTGCAGAAGGTCGCTTACATGAAATGCATCATCAAATTAGAACA
    TCTATTGGTGTATTAATGTTTATAACTGTTCCAGCTAGTATTGGAATTATGGCCTTGGCA
    CAACCATTATTCACAGTTTTCTATGGTTACGATCCAATTGTTTTAGGTCATGACCCTAAC
    CATGATGGTAGTCGCTTATTATTCTATTATGCGCCTGTAGCAATACTAATTTCACTATTA
    AGTGTAACGGCATCAATGTTACAAGGTATAGATAAACAGAAATTAACAGTTTATGTTATT
    TTAGCTTCTGTAGTTATCAAGTTAGCGTTAAACTATCCATTAATTATGTTATTCCATACA
    CCTGGTGCAATTTTAAGTACTAGTATCGCATTATTATTTGCAATTGGATGTAATTTCTAT
    ATTCTTAAAAAATATGCGAAGTTTAAATTCAGCTATAGTTGGATTCATTTTGCAAAAATA
    TTCTTGTATTCATTTATTATGATGCTAGGTGTAGAATTAGTATTTTTCCTTGCAAATCTA
    TTCTTAGAACCTACTAAACTTGGTTACTTAATCATTATTATTTTAGGTGTTACAGTAGGT
    ATTCTAATTTACGGTACAATCACAATTAAAACGCGTCTTGCGGACGAATTCTTAGGTGAA
    ATTCCTGAAAAATTAAGACGTAGAGTTAGGTTTTTACGATGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1662

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_1700 [new locus tag: SAUSA300_RS09285 ]
  • symbol: SAUSA300_1700
  • description: polysaccharide biosynthesis protein
  • length: 553
  • theoretical pI: 9.70447
  • theoretical MW: 61945.3
  • GRAVY: 0.712116

⊟Function[edit | edit source]

  • TIGRFAM:
    Cellular processes Cellular processes Sporulation and germination stage V sporulation protein B (TIGR02900; HMM-score: 122.7)
    and 2 more
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan murein biosynthesis integral membrane protein MurJ (TIGR01695; HMM-score: 43.5)
    Metabolism Transport and binding proteins Other MATE efflux family protein (TIGR00797; HMM-score: 21.5)
  • TheSEED  :
    • Probable cell division protein YtgP
    Cell Wall and Capsule Gram-Positive cell wall components Teichoic and lipoteichoic acids biosynthesis  Membrane protein involved in the export of O-antigen, teichoic acid lipoteichoic acids
  • PFAM:
    MviN_MATE (CL0222) Polysacc_synt; Polysaccharide biosynthesis protein (PF01943; HMM-score: 85)
    and 5 more
    MatE; MatE (PF01554; HMM-score: 40.5)
    MurJ; Lipid II flippase MurJ (PF03023; HMM-score: 36.8)
    BPD_transp_1 (CL0404) FtsX; FtsX-like permease C-terminal (PF02687; HMM-score: 14.6)
    no clan defined Phage_holin_10; Bacteriophage holin Pam3 gp28 family (PF23987; HMM-score: 13.7)
    Kinocilin; Kinocilin protein (PF15033; HMM-score: 7.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 14
  • DeepLocPro: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0.9999
    • Cell wall & surface Score: 0
    • Extracellular Score: 0
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.043737
    • TAT(Tat/SPI): 0.000861
    • LIPO(Sec/SPII): 0.002372
  • predicted transmembrane helices (TMHMM): 14

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MSESKEMVRGTFLITISILITKVLGVLFIIPFNYLIGGQENMAPFTYAYAPYNIAIAVATAGVPLAASKYVAKYNAIGAYKVSQKFYKSSFIVMSITGVLGFLVLYFLAPYISELTLARNIHDKNGWSVDDITWIIRIISMVVIFIPVLATWRGIFQGYKSMGPTAVSEVTEQIARVIFILIGSYLVLNVFDGSILLANGIATFAAAVGAIIGIFTLWYYWRKRKHNIDRMVESDYTDIDVSYGKMYKEIIAYSIPFVIVSLNYPLFNLVDQFTHNGALSLVGIPSQLQDIFFNMLNMSTNKIVMIPTSLSAGFAVSLIPYITKTFAEGRLHEMHHQIRTSIGVLMFITVPASIGIMALAQPLFTVFYGYDPIVLGHDPNHDGSRLLFYYAPVAILISLLSVTASMLQGIDKQKLTVYVILASVVIKLALNYPLIMLFHTPGAILSTSIALLFAIGCNFYILKKYAKFKFSYSWIHFAKIFLYSFIMMLGVELVFFLANLFLEPTKLGYLIIIILGVTVGILIYGTITIKTRLADEFLGEIPEKLRRRVRFLR

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • data available for JSNZ

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://fungenwikiserver.biologie.uni-greifswald.de/aureowiki/index.php?title=SAUSA300_1700&oldid=99802"