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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_1699 [new locus tag: SAUSA300_RS09280 ]
  • pan locus tag?: SAUPAN004431000
  • symbol: SAUSA300_1699
  • pan gene symbol?: —
  • synonym:
  • product: pseudouridine synthase family 1 protein

⊟Additional information (user-provided)[edit | edit source]

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_1699 [new locus tag: SAUSA300_RS09280 ]
  • symbol: SAUSA300_1699
  • product: pseudouridine synthase family 1 protein
  • replicon: chromosome
  • strand: -
  • coordinates: 1873763..1874458
  • length: 696
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

⊟Additional information (user-provided)[edit | edit source]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGAGAATAGATAAATTTTTAGCAAATATGGGCGTCGGTACACGTAATGAGGTCAAACAG
    TTACTTAAAAAAGGCCTTGTAAATGTCAACGAACAAGTAATTAAATCACCAAAAACACAT
    ATAGAACCAGAAAACGATAAGATAACAGTTCGTGGAGAATTAATAGAATACATTGAAAAT
    GTCTATATTATGTTAAATAAGCCAAAAGGTTATATATCAGCAACTGAAGATCATCATTCA
    AAAACCGTTATTGATTTAATACCTGAATACCAACATCTAAATATTTTTCCGGTTGGTCGT
    CTTGATAAAGATACAGAAGGGCTTTTATTGATAACGAATGATGGTGATTTTAATCATGAA
    TTAATGAGTCCAAACAAACATGTTTCTAAAAAATATGAAGTTATTTCAGCAAATCCTATC
    ACTGAAGACGACATTCAAGCTTTTAAAGAGGGTGTAACACTGACTGATGGCAAGGTTAAG
    CCTGCAATATTAACTTATATTGATAATCAAACGTCACACGTAACAATTTATGAAGGAAAA
    TATCATCAAGTTAAACGAATGTTTCATAGCATTCAAAATGAGGTATTACATTTAAGACGT
    ATCAAAATTGCTGATTTAGAATTGGACAGCAATTTAGATTCAGGAGAATATCGTTTATTA
    ACTGAAAATGATTTTGACAAATTAAATTATAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    696


⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_1699 [new locus tag: SAUSA300_RS09280 ]
  • symbol: SAUSA300_1699
  • description: pseudouridine synthase family 1 protein
  • length: 231
  • theoretical pI: 6.35246
  • theoretical MW: 26615.1
  • GRAVY: -0.545455

⊟Function[edit | edit source]

  • reaction:
    EC 5.4.99.-?  ExPASy
  • TIGRFAM:
    Genetic information processing Protein synthesis tRNA and rRNA base modification pseudouridine synthase (TIGR00093; EC 5.4.99.-; HMM-score: 152.9)
    and 4 more
    Genetic information processing Protein synthesis tRNA and rRNA base modification pseudouridine synthase, RluA family (TIGR00005; EC 5.4.99.-; HMM-score: 41.5)
    Unknown function General TlyA family rRNA methyltransferase/putative hemolysin (TIGR00478; HMM-score: 23.3)
    Genetic information processing Protein synthesis tRNA and rRNA base modification pseudouridine synthase Rlu family protein, TIGR01621 (TIGR01621; EC 5.4.99.-; HMM-score: 18.1)
    Genetic information processing Protein synthesis Ribosomal proteins: synthesis and modification ribosomal protein uS4 (TIGR01017; HMM-score: 17.2)
  • TheSEED  :
    • SSU rRNA pseudouridine(516) synthase (EC 5.4.99.19)
    Nucleosides and Nucleotides Nucleosides and Nucleotides - no subcategory Pseudouridine Metabolism  Ribosomal small subunit pseudouridine synthase A (EC 4.2.1.70)
  • PFAM:
    PseudoU_synth (CL0649) PseudoU_synth_2; RNA pseudouridylate synthase (PF00849; HMM-score: 80.2)
    and 2 more
    S4 (CL0492) S4; S4 domain (PF01479; HMM-score: 42.6)
    Acylphosphatase (CL0622) BLUF; Sensors of blue-light using FAD (PF04940; HMM-score: 12.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.67
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.15
    • Extracellular Score: 0.17
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.997
    • Cytoplasmic Membrane Score: 0.0022
    • Cell wall & surface Score: 0.0004
    • Extracellular Score: 0.0004
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.004965
    • TAT(Tat/SPI): 0.000675
    • LIPO(Sec/SPII): 0.000892
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Additional information (user-provided)[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MRIDKFLANMGVGTRNEVKQLLKKGLVNVNEQVIKSPKTHIEPENDKITVRGELIEYIENVYIMLNKPKGYISATEDHHSKTVIDLIPEYQHLNIFPVGRLDKDTEGLLLITNDGDFNHELMSPNKHVSKKYEVISANPITEDDIQAFKEGVTLTDGKVKPAILTYIDNQTSHVTIYEGKYHQVKRMFHSIQNEVLHLRRIKIADLELDSNLDSGEYRLLTENDFDKLNYK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • data available for JSNZ

⊟Additional information (user-provided)[edit | edit source]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Additional information (user-provided)[edit | edit source]

⊟Other information (user-provided)[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]


⊟Relevant publications[edit | edit source]

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