From AureoWiki
Jump to navigation Jump to search

NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_1550 [new locus tag: SAUSA300_RS08450 ]
  • pan locus tag?: SAUPAN004177000
  • symbol: SAUSA300_1550
  • pan gene symbol?: —
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_1550 [new locus tag: SAUSA300_RS08450 ]
  • symbol: SAUSA300_1550
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 1701715..1702431
  • length: 717
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGTCGCAATATGCAGAAATGAGCCTAGTGTACGATCAATTGACTCAAGATCAACCATAT
    GAAAAATGGTTTGAAATTGTAAAAAATCACTGCAAAGATGAATCAAATATTTTAGATATT
    GGATGCGGTACTGGTAGTTTAACAGTTCAATTAGAAGCTTTAGGTAATGTTACTGGTATG
    GATTTAAGTGTTGATATGTTGACTGTTGCAGCTCAAAAATCAGCAAATGTAAATTGGCTT
    GAAGGAGATATGACATCTTTTGATTTGCAACAACAATTTGATATCATCACTATTTTTTGT
    GACTCTCTAAATTATTTGCAAGATGAAACTGCAGTGATTGAAACATTCATCAATGTTTAT
    CATCATCTGACTGATTCAGGTGTATTTATTTTTGATGTACATACTGTTTATAAAATGATG
    ACTTTGTTTAATAATCAAAGTTATATTGATGATAAAGGGGACATTTTTTTAGCATGGGAT
    GCTGTGCAAGGAGATTTACCTTTAAGTGTTTATCATGATATGACATTTTTCATTCGACAT
    GAAGATGAAACATATTCAAGATTTGATGAGTCTCATTTTCAGAGAACATTTGATGAAAAG
    ACATATTTGTCTTGGTTAGCTCAAGTTGGTTTTAAACATGTTGAAACATTTACTGATTTT
    AATATAGATGAACATAATGAAGATGCAGAAAGATTGTTTTTCATTGCGAAAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    717

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_1550 [new locus tag: SAUSA300_RS08450 ]
  • symbol: SAUSA300_1550
  • description: hypothetical protein
  • length: 238
  • theoretical pI: 4.09468
  • theoretical MW: 27701.7
  • GRAVY: -0.262605

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Biotin malonyl-acyl carrier protein O-methyltransferase BioC (TIGR02072; EC 2.1.1.-; HMM-score: 48.3)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone ubiquinone/menaquinone biosynthesis methyltransferase (TIGR01934; EC 2.1.1.-; HMM-score: 42.8)
    and 17 more
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Chlorophyll and bacteriochlorphyll magnesium protoporphyrin O-methyltransferase (TIGR02021; EC 2.1.1.11; HMM-score: 34.9)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone 3-demethylubiquinone-9 3-O-methyltransferase (TIGR01983; EC 2.1.1.64; HMM-score: 33.5)
    Metabolism Amino acid biosynthesis Aspartate family methionine biosynthesis protein MetW (TIGR02081; HMM-score: 32.5)
    Genetic information processing Protein fate Protein modification and repair protein-(glutamine-N5) methyltransferase, release factor-specific (TIGR03534; EC 2.1.1.-; HMM-score: 25.5)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone demethylmenaquinone methyltransferase (TIGR02752; EC 2.1.1.163; HMM-score: 24.2)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Glutathione and analogs putative 4-mercaptohistidine N1-methyltranferase (TIGR04345; HMM-score: 22.1)
    Genetic information processing Protein synthesis tRNA and rRNA base modification ribosomal RNA small subunit methyltransferase A (TIGR00755; EC 2.1.1.182; HMM-score: 21.7)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin precorrin-6Y C5,15-methyltransferase (decarboxylating), CbiT subunit (TIGR02469; EC 2.1.1.132; HMM-score: 20.9)
    Unknown function Enzymes of unknown specificity putative methylase (TIGR00537; HMM-score: 17.8)
    Genetic information processing Transcription RNA processing 3' terminal RNA ribose 2'-O-methyltransferase Hen1 (TIGR04074; EC 2.1.1.-; HMM-score: 17.8)
    Genetic information processing Protein synthesis tRNA and rRNA base modification 3' terminal RNA ribose 2'-O-methyltransferase Hen1 (TIGR04074; EC 2.1.1.-; HMM-score: 17.8)
    Genetic information processing Protein fate Protein modification and repair methyltransferase, HemK family (TIGR00536; HMM-score: 17.3)
    methyltransferase, Rta_06860 family (TIGR04290; EC 2.1.1.-; HMM-score: 15.8)
    Genetic information processing Protein synthesis Ribosomal proteins: synthesis and modification ribosomal protein L11 methyltransferase (TIGR00406; EC 2.1.1.-; HMM-score: 15.7)
    Genetic information processing Protein synthesis Ribosomal proteins: synthesis and modification protein-(glutamine-N5) methyltransferase, ribosomal protein L3-specific (TIGR03533; EC 2.1.1.-; HMM-score: 14.8)
    Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides putative sugar O-methyltransferase (TIGR04371; EC 2.1.1.-; HMM-score: 14.3)
    2-ketoarginine methyltransferase (TIGR04543; EC 2.1.1.243; HMM-score: 11.8)
  • TheSEED  :
    • FIG145533: Methyltransferase (EC 2.1.1.-)
  • PFAM:
    NADP_Rossmann (CL0063) Methyltransf_25; Methyltransferase domain (PF13649; HMM-score: 57.2)
    Methyltransf_31; Methyltransferase domain (PF13847; HMM-score: 53.3)
    Methyltransf_11; Methyltransferase domain (PF08241; HMM-score: 46.2)
    and 14 more
    Methyltransf_12; Methyltransferase domain (PF08242; HMM-score: 44)
    NodS; Nodulation protein S (NodS) (PF05401; HMM-score: 42.9)
    Methyltransf_23; Methyltransferase domain (PF13489; HMM-score: 36)
    Ubie_methyltran; ubiE/COQ5 methyltransferase family (PF01209; HMM-score: 28.9)
    MetW; Methionine biosynthesis protein MetW (PF07021; HMM-score: 28.3)
    PrmA; Ribosomal protein L11 methyltransferase (PrmA) (PF06325; HMM-score: 21.4)
    MTS; Methyltransferase small domain (PF05175; HMM-score: 20.6)
    RrnaAD; Ribosomal RNA adenine dimethylase (PF00398; HMM-score: 18.4)
    CMAS; Mycolic acid cyclopropane synthetase (PF02353; HMM-score: 17.1)
    Methyltransf_32; Methyltransferase domain (PF13679; HMM-score: 17)
    HEXAPEP (CL0536) Fucokinase; L-fucokinase (PF07959; HMM-score: 14.4)
    NADP_Rossmann (CL0063) FtsJ; FtsJ-like methyltransferase (PF01728; HMM-score: 14.3)
    PCMT; Protein-L-isoaspartate(D-aspartate) O-methyltransferase (PCMT) (PF01135; HMM-score: 13.7)
    Methyltransf_8; Hypothetical methyltransferase (PF05148; HMM-score: 13.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.016832
    • TAT(Tat/SPI): 0.0002
    • LIPO(Sec/SPII): 0.000493
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MSQYAEMSLVYDQLTQDQPYEKWFEIVKNHCKDESNILDIGCGTGSLTVQLEALGNVTGMDLSVDMLTVAAQKSANVNWLEGDMTSFDLQQQFDIITIFCDSLNYLQDETAVIETFINVYHHLTDSGVFIFDVHTVYKMMTLFNNQSYIDDKGDIFLAWDAVQGDLPLSVYHDMTFFIRHEDETYSRFDESHFQRTFDEKTYLSWLAQVGFKHVETFTDFNIDEHNEDAERLFFIAKK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://fungenwikiserver.biologie.uni-greifswald.de/aureowiki/index.php?title=SAUSA300_1550&oldid=76022"