PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_1557 [new locus tag: SAUSA300_RS08485 ]
• pan locus tag^?: SAUPAN004184000
• symbol: SAUSA300_1557
• pan gene symbol^?: —
• synonym:
• product: HAD superfamily hydrolase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_1557 [new locus tag: SAUSA300_RS08485 ]
• symbol: SAUSA300_1557
• product: HAD superfamily hydrolase
• replicon: chromosome
• strand: -
• coordinates: 1706149..1706676
• length: 528
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3915265 NCBI
• RefSeq: YP_494252 NCBI
• BioCyc: see SAUSA300_RS08485
• MicrobesOnline: 1293072 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGGGTTTAGTTCGCAAGTTTTTTATGCCGAATTCATATGTTCAATCAATATTTCAAATT
  GATTTAGACAAGTTAGTGGACAAAGGCGTTAAAGGTATTATTACAGATTTAGATAATACG
  CTAGTAGGTTGGGATGTTAAAGAACCTACAGAACGTGTTAAAGCATGGTTTAAGGAAGCT
  AATGAAAAAGGAATCACTATTACAATCGTGTCTAATAATAATGAGTCTCGTGTTGCTAGT
  TTTAGTCAGCATTTAGACATCGATTTTATTTTTAAAGCGAGAAAGCCAATGGGGAAAGCG
  TTTGATAAAGCAATAACTAAGATGAATATCAGACCAGATCAAACTGTTGTTATAGGTGAC
  CAAATGCTTACTGATGTATTTGGTGGTAATCGTCGAGGTCTATATACAATTATGGTTGTT
  CCAGTTAAACGAACTGATGGCTTTATTACTAAGTTTAATAGATTAATTGAAAGGCGATTA
  TTACGTCATTTCAGTAAAAAAGGTTATATCACATGGGAGGAAAATTGA
  60
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  528
  

⊟Protein

⊟General

• locus tag: SAUSA300_1557 [new locus tag: SAUSA300_RS08485 ]
• symbol: SAUSA300_1557
• description: HAD superfamily hydrolase
• length: 175
• theoretical pI: 10.4084
• theoretical MW: 20219.4
• GRAVY: -0.305714

⊟Function

• reaction:
  EC 3.1.3.-?  ExPASy
• TIGRFAM:
  HAD phosphatase, family IIIA (TIGR01668; EC 3.1.3.-; HMM-score: 166.3)
  and 27 more
  Unknown function Enzymes of unknown specificity HAD hydrolase, family IIIA (TIGR01662; HMM-score: 81.4)
  HAD hydrolase, TIGR02253 family (TIGR02253; HMM-score: 40.4)
  Unknown function Enzymes of unknown specificity HAD hydrolase, family IIA (TIGR01460; HMM-score: 34.5)
  Unknown function Enzymes of unknown specificity Cof-like hydrolase (TIGR00099; HMM-score: 34.2)
  noncanonical pyrimidine nucleotidase, YjjG family (TIGR02254; EC 3.1.3.5; HMM-score: 31.3)
  phosphoglycolate/pyridoxal phosphate phosphatase family (TIGR01452; EC 3.1.3.18; HMM-score: 29.8)
  HAD hydrolase, REG-2-like, family IA (TIGR02252; HMM-score: 29)
  Unknown function Enzymes of unknown specificity HAD hydrolase, family IA, variant 1 (TIGR01549; HMM-score: 26.1)
  Unknown function Enzymes of unknown specificity HAD hydrolase, TIGR01457 family (TIGR01457; HMM-score: 23.6)
  Unknown function Enzymes of unknown specificity HAD hydrolase, family IA, variant 3 (TIGR01509; HMM-score: 23.4)
  histidinol-phosphate phosphatase domain (TIGR01656; HMM-score: 22.9)
  Unknown function Enzymes of unknown specificity HAD hydrolase, family IIB (TIGR01484; HMM-score: 22.2)
  HAD hydrolase, TIGR01459 family (TIGR01459; HMM-score: 22)
  Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase, YrbI family (TIGR01670; EC 3.1.3.45; HMM-score: 21.4)
  Unknown function Enzymes of unknown specificity HAD phosphoserine phosphatase-like hydrolase, family IB (TIGR01488; HMM-score: 21.2)
  Unknown function Enzymes of unknown specificity HAD hydrolase, TIGR01458 family (TIGR01458; HMM-score: 21)
  HAD phosphatase, family IIIC (TIGR01681; HMM-score: 18.8)
  mannosyl-3-phosphoglycerate phosphatase family (TIGR01486; EC 3.1.3.-; HMM-score: 18.1)
  HAD hydrolase, family IA, variant 2 (TIGR01493; HMM-score: 15.9)
  FkbH domain (TIGR01686; HMM-score: 15.9)
  sucrose-phosphate phosphatase subfamily (TIGR01482; EC 3.1.3.-; HMM-score: 15)
  mannosyl-3-phosphoglycerate phosphatase (TIGR02461; EC 3.1.3.70; HMM-score: 14.8)
  beta-phosphoglucomutase family hydrolase (TIGR02009; HMM-score: 14.5)
  haloacid dehalogenase, type II (TIGR01428; EC 3.8.1.2; HMM-score: 13.8)
  pyrimidine 5'-nucleotidase (TIGR01993; EC 3.1.3.5; HMM-score: 13.5)
  Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides beta-phosphoglucomutase (TIGR01990; EC 5.4.2.6; HMM-score: 12.1)
  sucrose phosphatase (TIGR01485; EC 3.1.3.24; HMM-score: 11.7)
• TheSEED  :
  • FIG001553: Hydrolase, HAD subfamily IIIA
• PFAM:
  HAD (CL0137) HAD_2; haloacid dehalogenase-like hydrolase (PF13419; HMM-score: 38.7)
  Hydrolase_like; HAD-hyrolase-like (PF13242; HMM-score: 35.2)
  Hydrolase_3; haloacid dehalogenase-like hydrolase (PF08282; HMM-score: 34)
  PGP_phosphatase; Mitochondrial PGP phosphatase (PF09419; HMM-score: 32.8)
  and 4 more
  Hydrolase; haloacid dehalogenase-like hydrolase (PF00702; HMM-score: 26.1)
  Hydrolase_6; haloacid dehalogenase-like hydrolase (PF13344; HMM-score: 16.7)
  Glyco_hydro_tim (CL0058) Cellulase; Cellulase (glycosyl hydrolase family 5) (PF00150; HMM-score: 13.5)
  HAD (CL0137) S6PP; Sucrose-6F-phosphate phosphohydrolase (PF05116; HMM-score: 13.3)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0.32
  • Cytoplasmic Membrane Score: 9.55
  • Cellwall Score: 0.12
  • Extracellular Score: 0.01
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.003078
  • TAT(Tat/SPI): 0.000147
  • LIPO(Sec/SPII): 0.000352
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87162418 NCBI
• RefSeq: YP_494252 NCBI
• UniProt: A0A0H2XJC7 UniProt

⊟Protein sequence

• MGLVRKFFMPNSYVQSIFQIDLDKLVDKGVKGIITDLDNTLVGWDVKEPTERVKAWFKEANEKGITITIVSNNNESRVASFSQHLDIDFIFKARKPMGKAFDKAITKMNIRPDQTVVIGDQMLTDVFGGNRRGLYTIMVVPVKRTDGFITKFNRLIERRLLRHFSKKGYITWEEN

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SAUSA300_1549 < SAUSA300_1550 < SAUSA300_1551 < SAUSA300_1552 < nadD < SAUSA300_1554 < aroE < SAUSA300_1556 < SAUSA300_1557 < mtnN

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications