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PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_1423 [new locus tag: SAUSA300_RS07765 ]
  • pan locus tag?: SAUPAN001439000
  • symbol: polA
  • pan gene symbol?: —
  • synonym:
  • product: phage related DNA polymerase family A

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_1423 [new locus tag: SAUSA300_RS07765 ]
  • symbol: polA
  • product: phage related DNA polymerase family A
  • replicon: chromosome
  • strand: -
  • coordinates: 1581398..1583350
  • length: 1953
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGAATATAGATATTGAAACATATAGCAGTAATGATATTTCGAAATGTGGTGCCTATAAA
    TACACAGAAGCTGAAGATTTCGAAATTTTAATTATAGCTTATTCAATAGATGGTGGAGCG
    ATTAGTGCGATTGACATGACTAAAGTAGATAATGAGCCTTTCCACGCTGATTTTGAGACG
    TTTAAAATTGCTCTTTTTGATCCTGCTGTAAAAAAGTATGCATTCAATGCTAATTTCGAA
    AGAACTTGTCTTGCTAAACATTTTAATAAACAGATGCCACCTGAAGAATGGATTTGCACA
    ATGGTTAATTCAATGCGTATTGGCTTACCTGCTTCGCTTGATAAAGTTGGAGAAGTTTTA
    AGACTACAAAACCAAAAAGATAAAGCAGGTAAAAATTTAATTCGTTATTTCTCTATACCT
    TGTAAACCAACAAAAGTTAATGGAGGAAGAACAAGAAACTTGCCTGAACATGATCTTGAA
    AAATGGCAACAATTTATAGATTACTGTATTCGAGATGTAGAAGTAGAAATGACGATTGCT
    CATAAAATTAAAGACTTTCCAGTAACTGCAATTGAACAAGCATATTGGGTTTTTGACCAA
    CATATAAACGACAGAGGTATTAAGCTTTCTAAATCATTGATGTTAGGAGCTAATGTGCTC
    GATAAGCAGAGTAAAGAAGAATTGCTTAATCAAGCTAAACATATAACAGGTTTAGAAAAT
    CCTAATAGTCCTACACAATTATTGGCTTGGTTAAAGGATGACCAAGGATTAGATATACCT
    AATTTACAAAAGAAAACGGTTCAGGAGTACTTAAAAGAAGCAACAGGAAAAGCTAAAAAA
    ATGCTAGAAATTAGATTGCAAATGTCTAAAACCAGTGTGAAAAAATACAACAAAATGCAT
    GACATGATGTGCAGTGATGAACGGGTAAGAGGTCTGTTTCAATTTTACGGTGCCGGTACT
    GGAAGATGGGCAGGTAGAGGTGTACAACTTCAGAATTTAACAAAGCATTATATTTCAGAT
    ACTGAATTAGAAATAGCAAGAGATCTTATTAAAGAACAACGTTTTGACGATTTAGATTTA
    TTACTCAATGTTCATCCTCAAGACTTATTAAGTCAATTAGTTAGGACGACATTTACTGCT
    GAAGAAGGTAATGAACTAGCAGTAAGTGATTTTTCTGCAATAGAGGCAAGAGTCATAGCA
    TGGTATGCAAAAGAACAATGGCGTTTAGATGTGTTCAACACACACGGAAAGATATATGAA
    GCATCGGCTTCTCAAATGTTTAATGTCCCGGTAGAAAGCATAACTAAAGGCGACCCTCTC
    AGACAAAAGGGAAAAGTGTCCGAATTAGCTTTAGGTTATCAAGGTGGCGCTGGAGCTTTA
    AAAGCGATGGGCGCATTGGAAATGGGCATTGAAGAAAATGAATTACAAGGTTTAGTTGAT
    AGTTGGCGTAACGCAAATCCTAACATAGTTAATTTTTGGAAGGCTTGCCAAGAGGCTGCA
    ATTAATACTGTGAAATCCCGAAAGACGCATCATACGCATGGAGTTAGATTTTATATGAAA
    AAAGGTTTTCTAATGATTGAACTGCCTAGTGGAAGAGCTTTAGCTTATCCAAAAGCTTCA
    GTTGGTGAAAATAGTTGGGGTAGTCAAGTTGTTGAATTTATGGGCTTAGATCTTAACCGT
    AAATGGTCAAAGTTAAAAACGTATGGTGGGAAGTTAGTCGAGAATATTGTTCAAGCAACT
    GCAAGGGATTTACTTGCGATTTCTATAGCTAGGCTTGAAGCATCAGGTTTTAAAATAGTT
    GGACATGTCCATGATGAAGTAATTGTAGAAATACCTAGAGGTTCAAATGGACTTAAGGAA
    ATCGAAACTATCATGAATAAGCCTGTCGATTGGGCAAAAGGATTGAATTTGAATAGTGAC
    GGATTTACTTCTCCGTTTTATATGAAGGATTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1953

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_1423 [new locus tag: SAUSA300_RS07765 ]
  • symbol: PolA
  • description: phage related DNA polymerase family A
  • length: 650
  • theoretical pI: 7.22452
  • theoretical MW: 73508.7
  • GRAVY: -0.390308

⊟Function[edit | edit source]

  • reaction:
    EC 2.7.7.7?  ExPASy
    DNA-directed DNA polymerase Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
  • TIGRFAM:
    Genetic information processing DNA metabolism DNA replication, recombination, and repair DNA polymerase I (TIGR00593; EC 2.7.7.7; HMM-score: 53.1)
  • TheSEED  :
    • DNA polymerase I (EC 2.7.7.7)
    DNA Metabolism DNA repair DNA Repair Base Excision  DNA polymerase I (EC 2.7.7.7)
    and 1 more
    DNA Metabolism DNA replication DNA-replication  DNA polymerase I (EC 2.7.7.7)
  • PFAM:
    no clan defined DNA_pol_A; DNA polymerase family A (PF00476; HMM-score: 87.1)
    and 1 more
    RNase_H (CL0219) DNA_pol_A_exo1; 3'-5' exonuclease (PF01612; HMM-score: 14.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.008616
    • TAT(Tat/SPI): 0.000629
    • LIPO(Sec/SPII): 0.001654
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MNIDIETYSSNDISKCGAYKYTEAEDFEILIIAYSIDGGAISAIDMTKVDNEPFHADFETFKIALFDPAVKKYAFNANFERTCLAKHFNKQMPPEEWICTMVNSMRIGLPASLDKVGEVLRLQNQKDKAGKNLIRYFSIPCKPTKVNGGRTRNLPEHDLEKWQQFIDYCIRDVEVEMTIAHKIKDFPVTAIEQAYWVFDQHINDRGIKLSKSLMLGANVLDKQSKEELLNQAKHITGLENPNSPTQLLAWLKDDQGLDIPNLQKKTVQEYLKEATGKAKKMLEIRLQMSKTSVKKYNKMHDMMCSDERVRGLFQFYGAGTGRWAGRGVQLQNLTKHYISDTELEIARDLIKEQRFDDLDLLLNVHPQDLLSQLVRTTFTAEEGNELAVSDFSAIEARVIAWYAKEQWRLDVFNTHGKIYEASASQMFNVPVESITKGDPLRQKGKVSELALGYQGGAGALKAMGALEMGIEENELQGLVDSWRNANPNIVNFWKACQEAAINTVKSRKTHHTHGVRFYMKKGFLMIELPSGRALAYPKASVGENSWGSQVVEFMGLDLNRKWSKLKTYGGKLVENIVQATARDLLAISIARLEASGFKIVGHVHDEVIVEIPRGSNGLKEIETIMNKPVDWAKGLNLNSDGFTSPFYMKD

⊟Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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