PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_RS07765 [old locus tag: SAUSA300_1423 ]
• pan locus tag^?: SAUPAN001439000
• symbol: SAUSA300_RS07765
• pan gene symbol^?: —
• synonym:
• product: DNA polymerase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_RS07765 [old locus tag: SAUSA300_1423 ]
• symbol: SAUSA300_RS07765
• product: DNA polymerase
• replicon: chromosome
• strand: -
• coordinates: 1581398..1583350
• length: 1953
• essential: unknown other strains

⊟Accession numbers

• Location: NC_007793 (1581398..1583350) NCBI
• BioCyc: SAUSA300_RS07765 BioCyc
• MicrobesOnline: see SAUSA300_1423

⊟Phenotype

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⊟DNA sequence

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  ATGAATATAGATATTGAAACATATAGCAGTAATGATATTTCGAAATGTGGTGCCTATAAA
  TACACAGAAGCTGAAGATTTCGAAATTTTAATTATAGCTTATTCAATAGATGGTGGAGCG
  ATTAGTGCGATTGACATGACTAAAGTAGATAATGAGCCTTTCCACGCTGATTTTGAGACG
  TTTAAAATTGCTCTTTTTGATCCTGCTGTAAAAAAGTATGCATTCAATGCTAATTTCGAA
  AGAACTTGTCTTGCTAAACATTTTAATAAACAGATGCCACCTGAAGAATGGATTTGCACA
  ATGGTTAATTCAATGCGTATTGGCTTACCTGCTTCGCTTGATAAAGTTGGAGAAGTTTTA
  AGACTACAAAACCAAAAAGATAAAGCAGGTAAAAATTTAATTCGTTATTTCTCTATACCT
  TGTAAACCAACAAAAGTTAATGGAGGAAGAACAAGAAACTTGCCTGAACATGATCTTGAA
  AAATGGCAACAATTTATAGATTACTGTATTCGAGATGTAGAAGTAGAAATGACGATTGCT
  CATAAAATTAAAGACTTTCCAGTAACTGCAATTGAACAAGCATATTGGGTTTTTGACCAA
  CATATAAACGACAGAGGTATTAAGCTTTCTAAATCATTGATGTTAGGAGCTAATGTGCTC
  GATAAGCAGAGTAAAGAAGAATTGCTTAATCAAGCTAAACATATAACAGGTTTAGAAAAT
  CCTAATAGTCCTACACAATTATTGGCTTGGTTAAAGGATGACCAAGGATTAGATATACCT
  AATTTACAAAAGAAAACGGTTCAGGAGTACTTAAAAGAAGCAACAGGAAAAGCTAAAAAA
  ATGCTAGAAATTAGATTGCAAATGTCTAAAACCAGTGTGAAAAAATACAACAAAATGCAT
  GACATGATGTGCAGTGATGAACGGGTAAGAGGTCTGTTTCAATTTTACGGTGCCGGTACT
  GGAAGATGGGCAGGTAGAGGTGTACAACTTCAGAATTTAACAAAGCATTATATTTCAGAT
  ACTGAATTAGAAATAGCAAGAGATCTTATTAAAGAACAACGTTTTGACGATTTAGATTTA
  TTACTCAATGTTCATCCTCAAGACTTATTAAGTCAATTAGTTAGGACGACATTTACTGCT
  GAAGAAGGTAATGAACTAGCAGTAAGTGATTTTTCTGCAATAGAGGCAAGAGTCATAGCA
  TGGTATGCAAAAGAACAATGGCGTTTAGATGTGTTCAACACACACGGAAAGATATATGAA
  GCATCGGCTTCTCAAATGTTTAATGTCCCGGTAGAAAGCATAACTAAAGGCGACCCTCTC
  AGACAAAAGGGAAAAGTGTCCGAATTAGCTTTAGGTTATCAAGGTGGCGCTGGAGCTTTA
  AAAGCGATGGGCGCATTGGAAATGGGCATTGAAGAAAATGAATTACAAGGTTTAGTTGAT
  AGTTGGCGTAACGCAAATCCTAACATAGTTAATTTTTGGAAGGCTTGCCAAGAGGCTGCA
  ATTAATACTGTGAAATCCCGAAAGACGCATCATACGCATGGAGTTAGATTTTATATGAAA
  AAAGGTTTTCTAATGATTGAACTGCCTAGTGGAAGAGCTTTAGCTTATCCAAAAGCTTCA
  GTTGGTGAAAATAGTTGGGGTAGTCAAGTTGTTGAATTTATGGGCTTAGATCTTAACCGT
  AAATGGTCAAAGTTAAAAACGTATGGTGGGAAGTTAGTCGAGAATATTGTTCAAGCAACT
  GCAAGGGATTTACTTGCGATTTCTATAGCTAGGCTTGAAGCATCAGGTTTTAAAATAGTT
  GGACATGTCCATGATGAAGTAATTGTAGAAATACCTAGAGGTTCAAATGGACTTAAGGAA
  ATCGAAACTATCATGAATAAGCCTGTCGATTGGGCAAAAGGATTGAATTTGAATAGTGAC
  GGATTTACTTCTCCGTTTTATATGAAGGATTAG
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⊟Protein

⊟General

• locus tag: SAUSA300_RS07765 [old locus tag: SAUSA300_1423 ]
• symbol: SAUSA300_RS07765
• description: DNA polymerase
• length: 650
• theoretical pI: 7.22452
• theoretical MW: 73508.7
• GRAVY: -0.390308

⊟Function

• reaction:
  EC 2.7.7.7?  ExPASy
  DNA-directed DNA polymerase Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
• TIGRFAM:
  Genetic information processing DNA metabolism DNA replication, recombination, and repair DNA polymerase I (TIGR00593; EC 2.7.7.7; HMM-score: 53.1)
• TheSEED: see SAUSA300_1423
• PFAM:
  no clan defined DNA_pol_A; DNA polymerase family A (PF00476; HMM-score: 87.1)
  and 1 more
  RNase_H (CL0219) DNA_pol_A_exo1; 3'-5' exonuclease (PF01612; HMM-score: 14.8)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.008616
  • TAT(Tat/SPI): 0.000629
  • LIPO(Sec/SPII): 0.001654
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: see SAUSA300_1423
• RefSeq: WP_001801657 NCBI
• UniProt: see SAUSA300_1423

⊟Protein sequence

• MNIDIETYSSNDISKCGAYKYTEAEDFEILIIAYSIDGGAISAIDMTKVDNEPFHADFETFKIALFDPAVKKYAFNANFERTCLAKHFNKQMPPEEWICTMVNSMRIGLPASLDKVGEVLRLQNQKDKAGKNLIRYFSIPCKPTKVNGGRTRNLPEHDLEKWQQFIDYCIRDVEVEMTIAHKIKDFPVTAIEQAYWVFDQHINDRGIKLSKSLMLGANVLDKQSKEELLNQAKHITGLENPNSPTQLLAWLKDDQGLDIPNLQKKTVQEYLKEATGKAKKMLEIRLQMSKTSVKKYNKMHDMMCSDERVRGLFQFYGAGTGRWAGRGVQLQNLTKHYISDTELEIARDLIKEQRFDDLDLLLNVHPQDLLSQLVRTTFTAEEGNELAVSDFSAIEARVIAWYAKEQWRLDVFNTHGKIYEASASQMFNVPVESITKGDPLRQKGKVSELALGYQGGAGALKAMGALEMGIEENELQGLVDSWRNANPNIVNFWKACQEAAINTVKSRKTHHTHGVRFYMKKGFLMIELPSGRALAYPKASVGENSWGSQVVEFMGLDLNRKWSKLKTYGGKLVENIVQATARDLLAISIARLEASGFKIVGHVHDEVIVEIPRGSNGLKEIETIMNKPVDWAKGLNLNSDGFTSPFYMKD

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications