PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_1421 [new locus tag: SAUSA300_RS07755 ]
• pan locus tag^?: SAUPAN001452000
• symbol: SAUSA300_1421
• pan gene symbol^?: —
• synonym:
• product: phiSLT ORF122-like protein, DNA polymerase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_1421 [new locus tag: SAUSA300_RS07755 ]
• symbol: SAUSA300_1421
• product: phiSLT ORF122-like protein, DNA polymerase
• replicon: chromosome
• strand: -
• coordinates: 1580799..1581200
• length: 402
• essential: unknown

⊟Accession numbers

• Gene ID: 3913228 NCBI
• RefSeq: YP_494118 NCBI
• BioCyc: see SAUSA300_RS07755
• MicrobesOnline: 1292936 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  
  ATGGCTAGAAGAAAAGTTATAAGAGTGCGTATCAAAGGAAAACTAATGACATTGAGAGAA
  GTTTCAGAAAAATATCATATATCTCCAGAACTTCTTAGATACAGATACAAACATAAAATG
  CGCGGCGATGAATTATTGTGTGGAAGAAAAGACTCAAAATCTAAAGATGAAGTTGAATAT
  ATGAAGAGTCAAATAAAAGATGAAGAAAAAGAGAGAGAAAAAATCAGAAAAAAAGCGATT
  TTGAACCTATACCAACGAAATGTGAGAGCGGAATATGAAGAAGAAAGAAAGAGAAGATTG
  AGACCATGGCTTTATGATGGAACGCCTCAAAAACATTCACGTGATCCGTACTGGTTCGAT
  GTCACTTATAACCAAATGTTCAAGAAATGGAGTGAAGCATAA
  60
  120
  180
  240
  300
  360
  402
  

⊟Protein

⊟General

• locus tag: SAUSA300_1421 [new locus tag: SAUSA300_RS07755 ]
• symbol: SAUSA300_1421
• description: phiSLT ORF122-like protein, DNA polymerase
• length: 133
• theoretical pI: 10.5044
• theoretical MW: 16573.1
• GRAVY: -1.36316

⊟Function

• TIGRFAM:
• TheSEED  :
  • Phage phi 11 orf21 protein homolog
• PFAM:
  no clan defined PVL_ORF50; PVL ORF-50-like family (PF07768; HMM-score: 180.1)
  and 1 more
  HTH (CL0123) Sigma70_r4; Sigma-70, region 4 (PF04545; HMM-score: 14.2)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.001928
  • TAT(Tat/SPI): 0.000442
  • LIPO(Sec/SPII): 0.000371
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87160354 NCBI
• RefSeq: YP_494118 NCBI
• UniProt: A0A0H2XEU7 UniProt

⊟Protein sequence

• MARRKVIRVRIKGKLMTLREVSEKYHISPELLRYRYKHKMRGDELLCGRKDSKSKDEVEYMKSQIKDEEKEREKIRKKAILNLYQRNVRAEYEEERKRRLRPWLYDGTPQKHSRDPYWFDVTYNQMFKKWSEA

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SAUSA300_1420 < SAUSA300_1421 < SAUSA300_1422 < polA < SAUSA300_1424 < SAUSA300_1425 < SAUSA300_1426 < SAUSA300_1427

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications