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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_0348 [new locus tag: SAUSA300_RS01845 ]
  • pan locus tag?: SAUPAN001890000
  • symbol: SAUSA300_0348
  • pan gene symbol?: tatA
  • synonym:
  • product: twin arginine-targeting protein translocase

tatA : twin-arginine translocase oligomerization subunit A [1]

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_0348 [new locus tag: SAUSA300_RS01845 ]
  • symbol: SAUSA300_0348
  • product: twin arginine-targeting protein translocase
  • replicon: chromosome
  • strand: -
  • coordinates: 400607..400831
  • length: 225
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    TTGATTATCATGATAACTAACACTTTTATTTTAGGCATCACAGGCCCAACAAGTCTTGTC
    GTCATTAGCATTATCGCTTTAATTATTTTTGGTCCGAAAAAATTACCACAATTTGGCCGT
    GCCATCGGTTCTACTTTAAAAGAATTTAAATCTGCAACAGAAGATTTAGATAAAGAGTCT
    CACGATACACCCAGTAAGGAATCGAAACAACAGCGAGAGCAATAG
    60
    120
    180
    225

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_0348 [new locus tag: SAUSA300_RS01845 ]
  • symbol: SAUSA300_0348
  • description: twin arginine-targeting protein translocase
  • length: 74
  • theoretical pI: 9.23936
  • theoretical MW: 8159.47
  • GRAVY: -0.0378378

⊟Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing Protein fate Protein and peptide secretion and trafficking twin arginine-targeting protein translocase, TatA/E family (TIGR01411; HMM-score: 67.9)
    and 1 more
    Genetic information processing Protein fate Protein and peptide secretion and trafficking twin arginine-targeting protein translocase TatB (TIGR01410; HMM-score: 34.3)
  • TheSEED  :
    • Twin-arginine translocation protein TatA
    Membrane Transport Protein translocation across cytoplasmic membrane Twin-arginine translocation system  Twin-arginine translocation protein TatA
  • PFAM:
    no clan defined TatA_B_E; mttA/Hcf106 family (PF02416; HMM-score: 70.9)
    and 1 more
    CPP1-like; Protein CHAPERONE-LIKE PROTEIN OF POR1-like (PF11833; HMM-score: 12.6)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.17
    • Cytoplasmic Membrane Score: 9.51
    • Cellwall Score: 0.16
    • Extracellular Score: 0.15
    • Internal Helix: 1
  • DeepLocPro: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0.9941
    • Cell wall & surface Score: 0
    • Extracellular Score: 0.0059
  • LocateP: N-terminally anchored (No CS)
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -0.5
    • N-terminally Anchored Score: 2
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.04799
    • TAT(Tat/SPI): 0.00091
    • LIPO(Sec/SPII): 0.003495
  • predicted transmembrane helices (TMHMM): 1

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MIIMITNTFILGITGPTSLVVISIIALIIFGPKKLPQFGRAIGSTLKEFKSATEDLDKESHDTPSKESKQQREQ

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: Fur* (repression) regulon
    Fur*(TF)important in Iron homeostasis; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Evelyne Turlin, Michel Débarbouillé, Katarzyna Augustyniak, Anne-Marie Gilles, Cécile Wandersman
    Staphylococcus aureus FepA and FepB proteins drive heme iron utilization in Escherichia coli.
    PLoS One: 2013, 8(2);e56529
    [PubMed:23437157] [WorldCat.org] [DOI] (I p)    Lalitha Biswas, Raja Biswas, Christiane Nerz, Knut Ohlsen, Martin Schlag, Tina Schäfer, Tobias Lamkemeyer, Anne-Kathrin Ziebandt, Klaus Hantke, Ralf Rosenstein, Friedrich Götz
    Role of the twin-arginine translocation pathway in Staphylococcus.
    J Bacteriol: 2009, 191(19);5921-9
    [PubMed:19633084] [WorldCat.org] [DOI] (I p)    Felicity Alcock, Ben C Berks
    New insights into the Tat protein transport cycle from characterizing the assembled Tat translocon.
    Mol Microbiol: 2022, 118(6);637-651
    [PubMed:36151601] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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