PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_0234 [new locus tag: SAUSA300_RS01240 ]
• pan locus tag^?: SAUPAN001105000
• symbol: SAUSA300_0234
• pan gene symbol^?: hmp
• synonym:
• product: putative flavohemoprotein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_0234 [new locus tag: SAUSA300_RS01240 ]
• symbol: SAUSA300_0234
• product: putative flavohemoprotein
• replicon: chromosome
• strand: -
• coordinates: 281209..282354
• length: 1146
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3915029 NCBI
• RefSeq: YP_492948 NCBI
• BioCyc: see SAUSA300_RS01240
• MicrobesOnline: 1291749 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

• 1
  61
  121
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  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  1141
  
  ATGCTTACAGAACAAGAGAAAGACATTATCAAACAAACGGTGCCTTTACTTAAAGAGAAA
  GGGACAGAAATTACGTCAATCTTTTATCCAAAAATGTTTAAAGCGCATCCTGAACTTTTA
  AACATGTTTAATCAAACGAACCAAAAACGAGGCATGCAATCTTCAGCATTAGCACAAGCT
  GTAATGGCCGCAGCGGTTAATATCGATAACTTAAGTGTTATTAAACCAGTCATTATGCCA
  GTCGCATATAAACACTGCGCACTACAAGTTTATGCTGAACATTATCCAATTGTGGGGAAA
  AATTTATTAAAAGCCATTCAAGACGTGACAGGATTAGAAGAAAATGACCCTGTCATTCAA
  GCTTGGGCAAAAGCATATGGCGTTATTGCGGATGTGTTCATCCAAATTGAAAAAGAAATT
  TATGATCAAATGATGTGGATTGGTTTTAAACCATTCAAAATTACCAATATTAAACAAGAA
  TCTGAAGACATTAAATCATTTACAGTTGAAACTGAAGAATATGACTTTAGTGAATTTACA
  CCAGGCCAATACATCACAGTTGATGTTTCTAGTGATAAACTTCCATATAGAGCTAAACGT
  CACTATTCTATCGTATCAGGTGAAAAAAACCATTTAACTTTTGGCGTTAAACGTGATGTC
  ACAACAGAACATGAAGGCGAAGTTTCAACAATTTTACACGATGAGATTAAAGAAGGCGAT
  ATGATTAATTTAGCTGCGCCTGTAGGTGGATTCGTATTAGAGAATACGACTGAACCACAA
  CTTTTCTTAGGTTCAGGTATTGGTGTTACACCTTTAGTAGCTATGTATGAAGCTGCCTCT
  GCCAAAGGTTTAGATACACAGATGGTTCAAGTTGCTGAAAATGAACAACATTTACCTTTC
  AAAGACAACTTCAACAGTATCGCAAGCCATCATGACAACGCTAAATTATATACACACTTA
  AAAGATAAACAAGGCTATATTGGTGCTGAAGAATTACAAGTATTTTTAGCAAATAAACCT
  GAAATTTATATCTGTGGTGGTACAAAATTCTTACAATCTATGATCGAAGCACTTAAATCT
  TTAAATTACGATATGGATCGCGTACACTACGAAACATTTATTCCAAGACTAAGCGTTGCA
  GTATAA
  60
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  1140
  1146
  

⊟Protein

⊟General

• locus tag: SAUSA300_0234 [new locus tag: SAUSA300_RS01240 ]
• symbol: SAUSA300_0234
• description: putative flavohemoprotein
• length: 381
• theoretical pI: 5.16615
• theoretical MW: 42906.8
• GRAVY: -0.212861

⊟Function

• TIGRFAM:
  Metabolism Energy metabolism Other phenylacetate-CoA oxygenase/reductase, PaaK subunit (TIGR02160; HMM-score: 56.5)
  and 1 more
  Metabolism Transport and binding proteins Cations and iron carrying compounds NADH:ubiquinone oxidoreductase, Na(+)-translocating, F subunit (TIGR01941; EC 1.6.5.-; HMM-score: 26.5)
• TheSEED  :
  • Flavohemoprotein (Hemoglobin-like protein) (Flavohemoglobin) (Nitric oxide dioxygenase) (EC 1.14.12.17)
  Stress Response Oxidative stress Glutaredoxins  Flavohemoprotein (Hemoglobin-like protein) (Flavohemoglobin) (Nitric oxide dioxygenase) (EC 1.14.12.17)
  and 2 more
  Stress Response Stress Response - no subcategory Bacterial hemoglobins  Flavohemoprotein (Hemoglobin-like protein) (Flavohemoglobin) (Nitric oxide dioxygenase) (EC 1.14.12.17)
  Stress Response Stress Response - no subcategory Flavohaemoglobin  Flavohemoprotein (Hemoglobin-like protein) (Flavohemoglobin) (Nitric oxide dioxygenase) (EC 1.14.12.17)
• PFAM:
  FAD_Lum_binding (CL0076) FAD_binding_6; Oxidoreductase FAD-binding domain (PF00970; HMM-score: 37.3)
  Globin (CL0090) Globin; Globin (PF00042; HMM-score: 32.1)
  and 1 more
  NAD_Ferredoxin (CL0091) NAD_binding_1; Oxidoreductase NAD-binding domain (PF00175; HMM-score: 22.4)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.00738
  • TAT(Tat/SPI): 0.001641
  • LIPO(Sec/SPII): 0.000377
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87162433 NCBI
• RefSeq: YP_492948 NCBI
• UniProt: A0A0H2XKK6 UniProt

⊟Protein sequence

• MLTEQEKDIIKQTVPLLKEKGTEITSIFYPKMFKAHPELLNMFNQTNQKRGMQSSALAQAVMAAAVNIDNLSVIKPVIMPVAYKHCALQVYAEHYPIVGKNLLKAIQDVTGLEENDPVIQAWAKAYGVIADVFIQIEKEIYDQMMWIGFKPFKITNIKQESEDIKSFTVETEEYDFSEFTPGQYITVDVSSDKLPYRAKRHYSIVSGEKNHLTFGVKRDVTTEHEGEVSTILHDEIKEGDMINLAAPVGGFVLENTTEPQLFLGSGIGVTPLVAMYEAASAKGLDTQMVQVAENEQHLPFKDNFNSIASHHDNAKLYTHLKDKQGYIGAEELQVFLANKPEIYICGGTKFLQSMIEALKSLNYDMDRVHYETFIPRLSVAV

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SAUSA300_0233 < SAUSA300_0234

⊟Regulation

• data available for N315

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications