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⊟Summary[edit | edit source]
- pan ID?: SAUPAN006371000
- symbol?: —
- synonym:
- description?: PTS mannose transporter subunit IIA
- PTS mannose transporter subunit IIA
- BglG family transcriptional antiterminator
- BglG family transcription antiterminator
- transcriptional antiterminator, BglG family
- transcriptional antiterminator BglG family protein
- transcription antiterminator
- Activator of the mannose operon (transcriptional antiterminator), BglG family
- transcription antiterminator, BglG family
- antiterminator transcriptional regulator
- deoR-like helix-turn-helix domain protein
- PRD domain-containing protein
- transcriptional antiterminator BglG
- transcriptional regulator
descriptions from strain specific annotations:
- strand?: +
- coordinates?: 6406819..6408693
- synteny block?: BlockID0050800
- occurrence?: in 100% of 34 strains
manR : mannose operon transcriptional activator ManR [1]
• the consensus ManR binding site consists of the inverted repeat sequence : 5'-ATTGCC-ACTAnTnAC-GGCAAT-3'
The transcriptional activator of the mannose phosphotransferase system (ManR) is a member of the BglG family of transcriptional activators which can exhibit complex regulation based on phosphorylation status. Extrapolating from the characterized MtlR system in Bacillus stenothermophilus, ManR can be phosphorylated at HIS-196 or HIS-266 by ManP resulting in decreased DNA binding and reduced mannose operon activation. When mannose is present, ManP preferentially phosphorylates mannose and dephosphorylates ManR resulting in transcriptional activation. ManR activation can be further enhanced by HPr-mediated phosphorylation of HIS-311 and HIS-370 resulting in increased DNA binding. This ability to calibrate transcriptional activator function based on the availability of substrates is a hallmark of the Escherichia coli antiterminator BglG with PTS regulation domains (PRD-I and PRD-II) serving analogous roles to the HIS-196/266 and HIS-311/370 regulation domains in ManR. Thus, BglG-family transcriptional activators are often misannotated as antiterminators due to their shared mechanism of regulatory control.
⊟Orthologs[edit | edit source]
04-02981:
SA2981_2579
08BA02176:
C248_2708
11819-97:
MS7_2645
6850:
RSAU_002483
71193:
ST398NM01_2690
ECT-R 2:
ECTR2_2494
ED133:
SAOV_2689
ED98:
SAAV_2710
HO 5096 0412:
SAEMRSA15_25450
JH1:
SaurJH1_2720
JH9:
SaurJH9_2664
JKD6008:
SAA6008_02699
JKD6159:
SAA6159_02536
JSNZ:
JSNZ_002630
LGA251:
SARLGA251_24120
M013:
M013TW_2621
MRSA252:
SAR2719
MSHR1132:
SAMSHR1132_24640
MSSA476:
SAS2526
Mu3:
SAHV_2624
Mu50:
SAV2640
MW2:
MW2561
RF122:
SAB2515
ST398:
SAPIG2690
T0131:
SAT0131_02906
TCH60:
HMPREF0772_10547
TW20:
SATW20_27780
USA300_TCH1516:
USA300HOU_2640
VC40:
SAVC_12080
⊟Genome Viewer[edit | edit source]
| COL | |
| N315 | |
| NCTC8325 | |
| Newman | |
| USA300_FPR3757 |
⊟Alignments[edit | edit source]
- alignment of orthologues: CLUSTAL format alignment by MAFFT L-INS-i (v7.307)
COL MLDRHLKLLQFFIKNPSKHISSNEIAKHVNVSNRTVRNDIHVINSNFMDDIIVSIKSKGY
N315 MLDRHLKLLQFFIKNPSKHISSNEIAEHVNVSNRTVRNDIHVINSNFMDDIIVSIKSKGY
NCTC8325 MLDRHLKLLQFFIKNPSKHISSNEIAKHVNVSNRTVRNDIHVINSNFMDDIIVSIKSKGY
Newman MLDRHLKLLQFFIKNPSKHISSNEIAKHVNVSNRTVRNDIHVINSNFMDDIIVSIKSKGY
USA300_FPR3757 MLDRHLKLLQFFIKNPSKHISSNEIAKHVNVSNRTVRNDIHVINSNFMDDIIVSIKSKGY
**************************:*********************************
COL QLNTSQYTLETITERYTHIQSYKEKLLLSMAYQLLMHNKSQTLQQLEQDYLLSKTVLNDY
N315 QLNTSQYTLETITERYTHIQSYKEKLLLSMAYQLLMHNKSQTLQQLEQDYLLSKTVLNDY
NCTC8325 QLNTSQYTLETITERYTHIQSYKEKLLLSMAYQLLMHNKSQTLQQLEQDYLLSKTVLNDY
Newman QLNTSQYTLETITERYTHIQSYKEKLLLSMAYQLLMHNKSQTLQQLEQDYLLSKTVLNDY
USA300_FPR3757 QLNTSQYTLETITERYTHIQSYKEKLLLSMAYQLLMHNKSQTLQQLEQDYLLSKTVLNDY
************************************************************
COL FVRIQQWCQKFNIALTIKKKQGIVVDGTDNDITNAIIHLNQLSSGHVHVEDLILNELPDS
N315 FVRIQQWCQKFNIVLTIKKKQGIVVDGTDNDITNAIIHLNQLSSGHVHVEDLILNELPDS
NCTC8325 FVRIQQWCQKFNIALTIKKKQGIVVDGTDNDITNAIIHLNQLSSGHVHVEDLILNELPDS
Newman FVRIQQWCQKFNIALTIKKKQGIVVDGTDNDITNAIIHLNQLSSGHVHVEDLILNELPDS
USA300_FPR3757 FVRIQQWCQKFNIALTIKKKQGIVVDGTDNDITNAIIHLNQLSSGHVHVEDLILNELPDS
*************.**********************************************
COL HQRMISHIIQETLQQHSIETTDIQIQQLLIHLILIIKRKQSLSELDTLNHDSKLISQSCI
N315 HQRMISHIIQETLQQHSIETTDIQIQQLLIHLILIIKRKQSLSELDTLNHDAKLISQSCI
NCTC8325 HQRMISHIIQETLQQHSIETTDIQIQQLLIHLILIIKRKQSLSELDTLNHDSKLISQSCI
Newman HQRMISHIIQETLQQHSIETTDIQIQQLLIHLILIIKRKQSLSELDTLNHDSKLISQSCI
USA300_FPR3757 HQRMISHIIQETLQQHSIETTDIQIQQLLIHLILIIKRKQSLSELDTLNHDSKLISQSCI
***************************************************:********
COL KQINERLGYDLNEKVINMFSFFIAYHFNKLDIGIERIFIQSYIDRLIELMQQRIHVPFNE
N315 KQINERLGYDLNEKVINMFSFFIAYHFNKLDIGIERIFIQSYIDRLIELMQQRIHVPFNE
NCTC8325 KQINERLGYDLNEKVINMFSFFIAYHFNKLDIGIERIFIQSYIDRLIELMQQRIHVPFNE
Newman KQINERLGYDLNEKVINMFSFFIAYHFNKLDIGIERIFIQSYIDRLIELMQQRIHVPFNE
USA300_FPR3757 KQINERLGYDLNEKVINMFSFFIAYHFNKLDIGIERIFIQSYIDRLIELMQQRIHVPFNE
************************************************************
COL DSILQQNLYNHFSKAYLRITQNIYLNNPLVIEIKKLYPFVFNTLFEAIDKLAIDTDIEMS
N315 DSILQQNLYNHFSKAYLRITQNIYLNNPLVIEIKKLYPFVFNTLFEAIDKLAIDTDIEMS
NCTC8325 DSILQQNLYNHFSKAYLRITQNIYLNNPLVIEIKKLYPFVFNTLFEAIDKLAIDTDIEMS
Newman DSILQQNLYNHFSKAYLRITQNIYLNNPLVIEIKKLYPFVFNTLFEAIDKLAIDTDIEMS
USA300_FPR3757 DSILQQNLYNHFSKAYLRITQNIYLNNPLVIEIKKLYPFVFNTLFEAIDKLAIDTDIEMS
************************************************************
COL EDEIAFLTIHFQAAIERRTKTQLNVVIACYYGLGVSNFLETKINNLSEELSVINTIKLEN
N315 EDEIAFLTIHFQAAIERRTKTQLNVVIACYYGLGVSNFLETKINNLSEELSVINTIKLES
NCTC8325 EDEIAFLTIHFQAAIERRTKTQLNVVIACYYGLGVSNFLETKINNLSEELSVINTIKLEN
Newman EDEIAFLTIHFQAAIERRTKTQLNVVIACYYGLGVSNFLETKINNLSEELSVINTIKLEN
USA300_FPR3757 EDEIAFLTIHFQAAIERRTKTQLNVVIACYYGLGVSNFLETKINNLSEELSVINTIKLEN
***********************************************************.
COL ITHYHFDNVDLLITTHDIPKQTLNILPKHLTTIKVAPLFSEDDRHKIRHVVKQKQNPVQA
N315 ITHYHFDNVDLLITTHDIPKQTLNILPKHLTTIKVAPLFSEDDRHKIRHVVKQKQNPVQA
NCTC8325 ITHYHFDNVDLLITTHDIPKQTLNILPKHLTTIKVAPLFSEDDRHKIRHVVKQKQNPVQA
Newman ITHYHFDNVDLLITTHDIPKQTLNILPKHLTTIKVAPLFSEDDRHKIRHVVKQKQNPVQA
USA300_FPR3757 ITHYHFDNVDLLITTHDIPKQTLNILPKHLTTIKVAPLFSEDDRHKIRHVVKQKQNPVQA
************************************************************
COL HHHMDTVNFLVVNTEQKSRHTVQIFEEAQKILQAHHAIVEGYIESALEREKSSSTYIGNF
N315 HHHMDTVNFLVVNTEQKSRHTVQIFEEAQKILQAHHAIVEGYIESALEREKSSSTYIGNF
NCTC8325 HHHMDTVNFLVVNTEQKSRHTVQIFEEAQKILQAHHAIVEGYIESALEREKSSSTYIGNF
Newman HHHMDTVNFLVVNTEQKSRHTVQIFEEAQKILQAHHAIVEGYIESALEREKSSSTYIGNF
USA300_FPR3757 HHHMDTVNFLVVNTEQKSRHTVQIFEEAQKILQAHHAIVEGYIESALEREKSSSTYIGNF
************************************************************
COL MAIPHGDPEKVLQSHVLIFRTKDVFPWRQHDVKLVFFLAISHKDKAFTKQMMQLIANLDD
N315 MAIPHGDPEKVLQSHVLIFRTKDVFPWRQHDVKLVFFLAISHKDKAFTKQMMQLIANLDD
NCTC8325 MAIPHGDPEKVLQSHVLIFRTKDVFPWRQHDVKLVFFLAISHKDKAFTKQMMQLIANLDD
Newman MAIPHGDPEKVLQSHVLIFRTKDVFPWRQHDVKLVFFLAISHKDKAFTKQMMQLIANLDD
USA300_FPR3757 MAIPHGDPEKVLQSHVLIFRTKDVFPWRQHDVKLVFFLAISHKDKAFTKQMMQLIANLDD
************************************************************
COL DSVNHLCSLDDHSLKQQLFEYLQE
N315 DSVNHLCSLDDHSLKQQLFEYLQE
NCTC8325 DSVNHLCSLDDHSLKQQLFEYLQE
Newman DSVNHLCSLDDHSLKQQLFEYLQE
USA300_FPR3757 DSVNHLCSLDDHSLKQQLFEYLQE
************************
- ↑ S A Henstra, M Tuinhof, R H Duurkens, G T Robillard
The Bacillus stearothermophilus mannitol regulator, MtlR, of the phosphotransferase system. A DNA-binding protein, regulated by HPr and iicbmtl-dependent phosphorylation.
J Biol Chem: 1999, 274(8);4754-63
[PubMed:9988713] [WorldCat.org] [DOI] (P p)Nicholas P Vitko, Melinda R Grosser, Dal Khatri, Thurlow R Lance, Anthony R Richardson
Expanded Glucose Import Capability Affords Staphylococcus aureus Optimized Glycolytic Flux during Infection.
mBio: 2016, 7(3);
[PubMed:27329749] [WorldCat.org] [DOI] (I e)