PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_2539 [new locus tag: NWMN_RS14590 ]
• pan locus tag^?: SAUPAN006371000
• symbol: NWMN_2539
• pan gene symbol^?: —
• synonym:
• product: transcriptional antiterminator BglG family protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_2539 [new locus tag: NWMN_RS14590 ]
• symbol: NWMN_2539
• product: transcriptional antiterminator BglG family protein
• replicon: chromosome
• strand: +
• coordinates: 2793703..2795577
• length: 1875
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5331579 NCBI
• RefSeq: YP_001333573 NCBI
• BioCyc:
• MicrobesOnline: 3708141 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGCTTGATAGACACTTAAAACTACTCCAGTTTTTTATTAAAAATCCATCAAAGCACATT
  AGTTCTAATGAAATTGCTAAACATGTCAATGTTTCAAACCGCACAGTTCGCAATGATATC
  CATGTTATCAATAGCAATTTTATGGATGATATCATCGTAAGTATTAAATCTAAAGGATAT
  CAATTAAATACATCTCAGTATACCTTAGAAACGATAACTGAACGTTACACGCATATTCAA
  TCATATAAAGAAAAACTATTATTATCTATGGCGTATCAACTGCTAATGCACAATAAATCG
  CAGACATTGCAACAACTAGAGCAAGATTATCTATTATCTAAAACTGTGCTCAACGACTAT
  TTCGTTCGAATTCAACAATGGTGTCAAAAATTTAATATCGCTTTAACAATCAAGAAAAAA
  CAAGGTATTGTGGTCGATGGAACAGATAATGATATCACTAATGCCATCATCCATTTAAAT
  CAATTATCATCAGGGCATGTACATGTTGAAGACCTAATTTTAAATGAATTACCTGATTCA
  CACCAAAGGATGATTTCGCATATTATACAAGAGACACTTCAACAACATAGTATTGAAACA
  ACTGATATTCAAATACAACAGCTTTTAATTCATCTCATTTTAATCATTAAACGTAAGCAA
  TCTTTAAGTGAGCTAGACACGTTAAACCACGATTCAAAATTGATATCTCAATCTTGCATT
  AAGCAAATCAACGAAAGATTAGGTTATGACTTGAACGAAAAAGTAATCAATATGTTTTCA
  TTCTTTATTGCTTATCATTTTAATAAACTCGATATTGGCATAGAACGTATATTTATACAA
  AGTTATATTGATCGCTTAATCGAATTGATGCAGCAACGTATTCATGTACCTTTCAATGAA
  GATTCGATTTTACAACAAAATTTATATAATCATTTTAGTAAAGCATATTTACGTATCACT
  CAAAACATTTATTTAAACAATCCTTTAGTTATAGAGATTAAAAAACTCTATCCCTTTGTG
  TTTAACACACTTTTTGAAGCGATTGATAAATTAGCAATTGATACTGATATAGAGATGAGC
  GAAGATGAAATTGCATTTTTAACAATTCATTTTCAAGCTGCCATCGAGCGTCGTACCAAA
  ACACAATTAAATGTAGTCATTGCTTGTTATTATGGTCTAGGTGTGTCGAATTTTTTAGAA
  ACTAAAATAAATAACTTATCCGAAGAGTTGTCAGTGATAAATACGATTAAACTAGAAAAT
  ATAACACACTATCACTTTGACAATGTAGATTTATTAATAACGACCCATGATATTCCTAAA
  CAAACGCTAAACATACTTCCTAAGCACCTGACGACTATCAAGGTTGCACCATTATTTTCT
  GAAGATGATCGTCACAAAATCAGACACGTTGTGAAGCAAAAGCAAAATCCGGTTCAAGCA
  CATCATCATATGGACACTGTCAATTTCCTTGTCGTAAATACTGAACAAAAATCACGGCAT
  ACTGTGCAAATTTTTGAAGAAGCTCAAAAAATATTACAAGCACATCATGCGATTGTAGAA
  GGATATATCGAATCAGCTTTAGAGCGTGAGAAATCATCTTCTACATACATAGGTAATTTT
  ATGGCGATTCCTCATGGAGATCCTGAAAAAGTTTTACAATCACATGTGCTTATTTTTCGC
  ACAAAAGATGTTTTTCCTTGGCGACAACACGATGTCAAACTTGTCTTTTTCTTAGCGATT
  TCGCATAAAGACAAAGCTTTTACAAAACAAATGATGCAATTAATTGCTAACTTGGATGAT
  GATTCAGTGAACCATTTATGTAGTTTAGATGATCATAGTTTAAAGCAACAGTTATTTGAA
  TACTTACAAGAATAA
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⊟Protein

⊟General

• locus tag: NWMN_2539 [new locus tag: NWMN_RS14590 ]
• symbol: NWMN_2539
• description: transcriptional antiterminator BglG family protein
• length: 624
• theoretical pI: 6.9121
• theoretical MW: 72978.3
• GRAVY: -0.255929

⊟Function

• TIGRFAM:
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids PTS system, fructose subfamily, IIA component (TIGR00848; EC 2.7.1.69; HMM-score: 42.4)
  Signal transduction Signal transduction PTS PTS system, fructose subfamily, IIA component (TIGR00848; EC 2.7.1.69; HMM-score: 42.4)
  and 2 more
  Signal transduction Signal transduction PTS PTS IIA-like nitrogen-regulatory protein PtsN (TIGR01419; HMM-score: 23.3)
  Unknown function General PRD domain protein, EF_0829/AHA_3910 family (TIGR03582; HMM-score: 17.2)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  PRD (CL0166) PRD; PRD domain (PF00874; HMM-score: 75.8)
  PTase-anion_tr (CL0340) PTS_EIIA_2; Phosphoenolpyruvate-dependent sugar phosphotransferase system, EIIA 2 (PF00359; HMM-score: 66.4)
  and 10 more
  HTH (CL0123) HTH_11; HTH domain (PF08279; HMM-score: 51.3)
  HTH_Mga; M protein trans-acting positive regulator (MGA) HTH domain (PF08280; HMM-score: 33.1)
  PuR_N; Bacterial purine repressor, N-terminal (PF09182; HMM-score: 24.3)
  Mga; Mga helix-turn-helix domain (PF05043; HMM-score: 22.3)
  HTH_DeoR; DeoR-like helix-turn-helix domain (PF08220; HMM-score: 21.4)
  Phosphatase (CL0031) PTS_IIB; PTS system, Lactose/Cellobiose specific IIB subunit (PF02302; HMM-score: 19.9)
  HTH (CL0123) Put_DNA-bind_N; Putative DNA-binding protein N-terminus (PF06971; HMM-score: 18.3)
  HTH_24; Winged helix-turn-helix DNA-binding (PF13412; HMM-score: 14.7)
  HrcA_DNA-bdg; Winged helix-turn-helix transcription repressor, HrcA DNA-binding (PF03444; HMM-score: 12.4)
  no clan defined DUF745; Protein of unknown function (DUF745) (PF05335; HMM-score: 9.2)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:
• genes regulated by ManR, TF important in Mannose utilization: in N315

⊟Localization

• PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 2.5
  • Cytoplasmic Membrane Score: 2.5
  • Cellwall Score: 2.5
  • Extracellular Score: 2.5
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.004381
  • TAT(Tat/SPI): 0.00039
  • LIPO(Sec/SPII): 0.000603
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151222751 NCBI
• RefSeq: YP_001333573 NCBI
• UniProt: A0A0H3KAU5 UniProt

⊟Protein sequence

• MLDRHLKLLQFFIKNPSKHISSNEIAKHVNVSNRTVRNDIHVINSNFMDDIIVSIKSKGYQLNTSQYTLETITERYTHIQSYKEKLLLSMAYQLLMHNKSQTLQQLEQDYLLSKTVLNDYFVRIQQWCQKFNIALTIKKKQGIVVDGTDNDITNAIIHLNQLSSGHVHVEDLILNELPDSHQRMISHIIQETLQQHSIETTDIQIQQLLIHLILIIKRKQSLSELDTLNHDSKLISQSCIKQINERLGYDLNEKVINMFSFFIAYHFNKLDIGIERIFIQSYIDRLIELMQQRIHVPFNEDSILQQNLYNHFSKAYLRITQNIYLNNPLVIEIKKLYPFVFNTLFEAIDKLAIDTDIEMSEDEIAFLTIHFQAAIERRTKTQLNVVIACYYGLGVSNFLETKINNLSEELSVINTIKLENITHYHFDNVDLLITTHDIPKQTLNILPKHLTTIKVAPLFSEDDRHKIRHVVKQKQNPVQAHHHMDTVNFLVVNTEQKSRHTVQIFEEAQKILQAHHAIVEGYIESALEREKSSSTYIGNFMAIPHGDPEKVLQSHVLIFRTKDVFPWRQHDVKLVFFLAISHKDKAFTKQMMQLIANLDDDSVNHLCSLDDHSLKQQLFEYLQE

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: NWMN_2539 > NWMN_2540 > NWMN_2541

⊟Regulation

• regulator: CcpA regulon

  CcpA

  (TF)

  important in Carbon catabolism; RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications