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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_02974
  • pan locus tag?: SAUPAN006371000
  • symbol: SAOUHSC_02974
  • pan gene symbol?: —
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_02974
  • symbol: SAOUHSC_02974
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 2736110..2737984
  • length: 1875
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGCTTGATAGACACTTAAAACTACTCCAGTTTTTTATTAAAAATCCATCAAAGCACATT
    AGTTCTAATGAAATTGCTAAACATGTCAATGTTTCAAACCGCACAGTTCGCAATGATATC
    CATGTTATCAATAGCAATTTTATGGATGATATCATCGTAAGTATTAAATCTAAAGGATAT
    CAATTAAATACATCTCAGTATACCTTAGAAACGATAACTGAACGTTACACGCATATTCAA
    TCATATAAAGAAAAACTATTATTATCTATGGCGTATCAACTGCTAATGCACAATAAATCG
    CAGACATTGCAACAACTAGAGCAAGATTATCTATTATCTAAAACTGTGCTCAACGACTAT
    TTCGTTCGAATTCAACAATGGTGTCAAAAATTTAATATCGCTTTAACAATCAAGAAAAAA
    CAAGGTATTGTGGTCGATGGAACAGATAATGATATCACTAATGCCATCATCCATTTAAAT
    CAATTATCATCAGGGCATGTACATGTTGAAGACCTAATTTTAAATGAATTACCTGATTCA
    CACCAAAGGATGATTTCGCATATTATACAAGAGACACTTCAACAACATAGTATTGAAACA
    ACTGATATTCAAATACAACAGCTTTTAATTCATCTCATTTTAATCATTAAACGTAAGCAA
    TCTTTAAGTGAGCTAGACACGTTAAACCACGATTCAAAATTGATATCTCAATCTTGCATT
    AAGCAAATCAACGAAAGATTAGGTTATGACTTGAACGAAAAAGTAATCAATATGTTTTCA
    TTCTTTATTGCTTATCATTTTAATAAACTCGATATTGGCATAGAACGTATATTTATACAA
    AGTTATATTGATCGCTTAATCGAATTGATGCAGCAACGTATTCATGTACCTTTCAATGAA
    GATTCGATTTTACAACAAAATTTATATAATCATTTTAGTAAAGCATATTTACGTATCACT
    CAAAACATTTATTTAAACAATCCTTTAGTTATAGAGATTAAAAAACTCTATCCCTTTGTG
    TTTAACACACTTTTTGAAGCGATTGATAAATTAGCAATTGATACTGATATAGAGATGAGC
    GAAGATGAAATTGCATTTTTAACAATTCATTTTCAAGCTGCCATCGAGCGTCGTACCAAA
    ACACAATTAAATGTAGTCATTGCTTGTTATTATGGTCTAGGTGTGTCGAATTTTTTAGAA
    ACTAAAATAAATAACTTATCCGAAGAGTTGTCAGTGATAAATACGATTAAACTAGAAAAT
    ATAACACACTATCACTTTGACAATGTAGATTTATTAATAACGACCCATGATATTCCTAAA
    CAAACGCTAAACATACTTCCTAAGCACCTGACGACTATCAAGGTTGCACCATTATTTTCT
    GAAGATGATCGTCACAAAATCAGACACGTTGTGAAGCAAAAGCAAAATCCGGTTCAAGCA
    CATCATCATATGGACACTGTCAATTTCCTTGTCGTAAATACTGAACAAAAATCACGGCAT
    ACTGTGCAAATTTTTGAAGAAGCTCAAAAAATATTACAAGCACATCATGCGATTGTAGAA
    GGATATATCGAATCAGCTTTAGAGCGTGAGAAATCATCTTCTACATACATAGGTAATTTT
    ATGGCGATTCCTCATGGAGATCCTGAAAAAGTTTTACAATCACATGTGCTTATTTTTCGC
    ACAAAAGATGTTTTTCCTTGGCGACAACACGATGTCAAACTTGTCTTTTTCTTAGCGATT
    TCGCATAAAGACAAAGCTTTTACAAAACAAATGATGCAATTAATTGCTAACTTGGATGAT
    GATTCAGTGAACCATTTATGTAGTTTAGATGATCATAGTTTAAAGCAACAGTTATTTGAA
    TACTTACAAGAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1875

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_02974
  • symbol: SAOUHSC_02974
  • description: hypothetical protein
  • length: 624
  • theoretical pI: 6.9121
  • theoretical MW: 72978.3
  • GRAVY: -0.255929

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids PTS system, fructose subfamily, IIA component (TIGR00848; EC 2.7.1.69; HMM-score: 42.4)
    Signal transduction Signal transduction PTS PTS system, fructose subfamily, IIA component (TIGR00848; EC 2.7.1.69; HMM-score: 42.4)
    and 2 more
    Signal transduction Signal transduction PTS PTS IIA-like nitrogen-regulatory protein PtsN (TIGR01419; HMM-score: 23.3)
    Unknown function General PRD domain protein, EF_0829/AHA_3910 family (TIGR03582; HMM-score: 17.2)
  • TheSEED  :
    • Activator of the mannose operon (transcriptional antiterminator), BglG family
    Carbohydrates Monosaccharides Mannose Metabolism  Activator of the mannose operon (transcriptional antiterminator), BglG family
  • PFAM:
    PRD (CL0166) PRD; PRD domain (PF00874; HMM-score: 75.8)
    PTase-anion_tr (CL0340) PTS_EIIA_2; Phosphoenolpyruvate-dependent sugar phosphotransferase system, EIIA 2 (PF00359; HMM-score: 66.4)
    and 10 more
    HTH (CL0123) HTH_11; HTH domain (PF08279; HMM-score: 51.3)
    HTH_Mga; M protein trans-acting positive regulator (MGA) HTH domain (PF08280; HMM-score: 33.1)
    PuR_N; Bacterial purine repressor, N-terminal (PF09182; HMM-score: 24.3)
    Mga; Mga helix-turn-helix domain (PF05043; HMM-score: 22.3)
    HTH_DeoR; DeoR-like helix-turn-helix domain (PF08220; HMM-score: 21.4)
    Phosphatase (CL0031) PTS_IIB; PTS system, Lactose/Cellobiose specific IIB subunit (PF02302; HMM-score: 19.9)
    HTH (CL0123) Put_DNA-bind_N; Putative DNA-binding protein N-terminus (PF06971; HMM-score: 18.3)
    HTH_24; Winged helix-turn-helix DNA-binding (PF13412; HMM-score: 14.7)
    HrcA_DNA-bdg; Winged helix-turn-helix transcription repressor, HrcA DNA-binding (PF03444; HMM-score: 12.4)
    no clan defined DUF745; Protein of unknown function (DUF745) (PF05335; HMM-score: 9.2)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors: HPr (phosphocarrier protein); ManP (mannose-specific enzyme IIBCA PTS component)
  • genes regulated by ManR, TF important in Mannose utilization [1]
    activation
    manP* > pmi*

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.004381
    • TAT(Tat/SPI): 0.00039
    • LIPO(Sec/SPII): 0.000603
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MLDRHLKLLQFFIKNPSKHISSNEIAKHVNVSNRTVRNDIHVINSNFMDDIIVSIKSKGYQLNTSQYTLETITERYTHIQSYKEKLLLSMAYQLLMHNKSQTLQQLEQDYLLSKTVLNDYFVRIQQWCQKFNIALTIKKKQGIVVDGTDNDITNAIIHLNQLSSGHVHVEDLILNELPDSHQRMISHIIQETLQQHSIETTDIQIQQLLIHLILIIKRKQSLSELDTLNHDSKLISQSCIKQINERLGYDLNEKVINMFSFFIAYHFNKLDIGIERIFIQSYIDRLIELMQQRIHVPFNEDSILQQNLYNHFSKAYLRITQNIYLNNPLVIEIKKLYPFVFNTLFEAIDKLAIDTDIEMSEDEIAFLTIHFQAAIERRTKTQLNVVIACYYGLGVSNFLETKINNLSEELSVINTIKLENITHYHFDNVDLLITTHDIPKQTLNILPKHLTTIKVAPLFSEDDRHKIRHVVKQKQNPVQAHHHMDTVNFLVVNTEQKSRHTVQIFEEAQKILQAHHAIVEGYIESALEREKSSSTYIGNFMAIPHGDPEKVLQSHVLIFRTKDVFPWRQHDVKLVFFLAISHKDKAFTKQMMQLIANLDDDSVNHLCSLDDHSLKQQLFEYLQE

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [2] [3]
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CcpA* regulon
    CcpA*(TF)important in Carbon catabolism; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ 1.0 1.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)
  2. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  3. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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