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⊟Summary[edit | edit source]
- pan ID?: SAUPAN002250000
- symbol?: tilS
- synonym:
- description?: tRNA(Ile)-lysidine synthetase
- tRNA(Ile)-lysidine synthetase
- tRNA lysidine(34) synthetase TilS
- tRNA(Ile)-lysidine synthetase, putative
- protein TilS
- tRNA(Ile)-lysidine synthase
- tRNA(Ile)-lysidine synthase (tRNA(Ile)-lysidinesynthetase) (tRNA(Ile)-2-lysyl-cytidine synthase)
descriptions from strain specific annotations:
- strand?: +
- coordinates?: 2757107..2758402
- synteny block?: BlockID0015490
- occurrence?: in 100% of 34 strains
tilS : tRNA cytidine(34) O(2')-lysyltransferase [1]
Several transfer RNAs require modifications to provide maximal fidelity during protein synthesis. TilS (alt: tRNA(Ile)-lysidine synthetase) modifies cytidines at tRNA position 34 with lysine generating 2'-lysylcytidine ("lys2'C"). This modification is only required for the atypical tRNAICAU which shares an anticodon sequence with tRNA-Met. Modification of the anticodon cytidine(34) with lysine alongside TilS-mediated proofreading of isoacceptor stem charging with isoleucine allows this modified isoacceptor-anticodon to recognize the isoleucine codon AUA.
⊟Orthologs[edit | edit source]
04-02981:
SA2981_0484
08BA02176:
C248_0565
11819-97:
MS7_0486 (tilS)
6850:
RSAU_000448 (tilS)
71193:
ST398NM01_0575
ECT-R 2:
ECTR2_449 (tilS)
ED133:
SAOV_0531
ED98:
SAAV_0456
HO 5096 0412:
SAEMRSA15_04350
JH1:
SaurJH1_0544
JH9:
SaurJH9_0531
JKD6008:
SAA6008_00514 (tilS)
JKD6159:
SAA6159_00460 (tilS)
JSNZ:
JSNZ_000449 (tilS)
LGA251:
SARLGA251_04430 (tilS)
M013:
M013TW_0481
MRSA252:
SAR0510
MSHR1132:
SAMSHR1132_04530
MSSA476:
SAS0466
Mu3:
SAHV_0506
Mu50:
SAV0509
MW2:
MW0464
RF122:
SAB0458
ST398:
SAPIG0575
T0131:
SAT0131_00555 (menC)
TCH60:
HMPREF0772_10010
TW20:
SATW20_05770 (tilS)
USA300_TCH1516:
USA300HOU_0503
VC40:
SAVC_02130
⊟Genome Viewer[edit | edit source]
| COL | |
| N315 | |
| NCTC8325 | |
| Newman | |
| USA300_FPR3757 |
⊟Alignments[edit | edit source]
- alignment of orthologues: CLUSTAL format alignment by MAFFT L-INS-i (v7.307)
COL MQLNSNGWHVDDHIVVAVSTGIDSMCLLYQLLNDYKDSYRKLTCLHVNHGVRSASIEEAR
N315 MQLNSNGWHVDDHIVVAVSTGIDSMCLLYQLLNDYKDSYRKLTCLHVNHGVRSASIEEAR
NCTC8325 MQLNSNGWHVDDHIVVAVSTGIDSMCLLYQLLNDYKDSYRKLTCLHVNHGVRSASIEEAR
Newman MQLNSNGWHVDDHIVVAVSTGIDSMCLLYQLLNDYKDSYRKLTCLHVNHGVRSASIEEAR
USA300_FPR3757 MQLNSNGWHVDDHIVVAVSTGIDSMCLLYQLLNDYKDSYRKLTCLHVNHGVRSASIEEAR
************************************************************
COL FLEAYCERHHIDLHIKKLDLSHSLDRNNSIQNEARIKRYEWFDEMMNVLEADVLLTAHHL
N315 FLEAYCERHHIDLHIKKLDLSHSLDRNNSIQNEARIKRYEWFDEMMNVLEADVLLTAHHL
NCTC8325 FLEAYCERHHIDLHIKKLDLSHSLDRNNSIQNEARIKRYEWFDEMMNVLEADVLLTAHHL
Newman FLEAYCERHHIDLHIKKLDLSHSLDRNNSIQNEARIKRYEWFDEMMNVLEADVLLTAHHL
USA300_FPR3757 FLEAYCERHHIDLHIKKLDLSHSLDRNNSIQNEARIKRYEWFDEMMNVLEADVLLTAHHL
************************************************************
COL DDQLETIMYRIFNGKSTRNKLGFDELSKRKGYQIYRPLLAVSKKEIKQFQERYHIPYFED
N315 DDQLETIMYRIFNGKSTRNKLGFDELSKRKGYQIYRPLLAVSKKEIKQFQERYHIPYFED
NCTC8325 DDQLETIMYRIFNGKSTRNKLGFDELSKRKGYQIYRPLLAVSKKEIKQFQERYHIPYFED
Newman DDQLETIMYRIFNGKSTRNKLGFDELSKRKGYQIYRPLLAVSKKEIKQFQERYHIPYFED
USA300_FPR3757 DDQLETIMYRIFNGKSTRNKLGFDELSKRKGYQIYRPLLAVSKKEIKQFQERYHIPYFED
************************************************************
COL ESNKDNKYVRNDIRNRIIPAIDENNQLKVSHLLKLKQWHDEQYDILQYSAKQFIQEFVKF
N315 ESNKDNKYIRNDIRNRIIPAIDENNQLKVSHLLKLKQWHDEQYDILQYSAKQFIQEFVKF
NCTC8325 ESNKDNKYVRNDIRNRIIPAIDENNQLKVSHLLKLKQWHDEQYDILQYSAKQFIQEFVKF
Newman ESNKDNKYVRNDIRNRIIPAIDENNQLKVSHLLKLKQWHDEQYDILQYSAKQFIQEFVKF
USA300_FPR3757 ESNKDNKYVRNDIRNRIIPAIDENNQLKVSHLLKLKQWHDEQYDILQYSAKQFIQEFVKF
********:***************************************************
COL DEQSKYLEVSRQAFNNLPNSLKMVVLDCLLSKYYELFNISAKTYEEWFKQFSSKKAQFSI
N315 DEQSKYLEVSRQAFNNLPNSLKMVVLDCLLSKYYELFNISAKTYEEWFKQFSSKKAQFSI
NCTC8325 DEQSKYLEVSRQAFNNLPNSLKMVVLDCLLSKYYELFNISAKTYEEWFKQFSSKKAQFSI
Newman DEQSKYLEVSRQAFNNLPNSLKMVVLDCLLSKYYELFNISAKTYEEWFKQFSSKKAQFSI
USA300_FPR3757 DEQSKYLEVSRQAFNNLPNSLKMVVLDCLLSKYYELFNISAKTYEEWFKQFSSKKAQFSI
************************************************************
COL NLTDKWIIQIAYGKLIIMAKNNGDTYFRVQTIKKPGNYIFNKYRLEIHSNLPKCLFPLTV
N315 NLTDKWIIQIAYGKLIIMAKNNGDTYFRVQTIKKPGNYFFNKYRLEIHSNLPKCLFPLTV
NCTC8325 NLTDKWIIQIAYGKLIIMAKNNGDTYFRVQTIKKPGNYIFNKYRLEIHSNLPKCLFPLTV
Newman NLTDKWIIQIAYGKLIIMAKNNGDTYFRVQTIKKPGNYIFNKYRLEIHSNLPKCLFPLTV
USA300_FPR3757 NLTDKWIIQIAYGKLIIMAKNNGDTYFRVQTIKKPGNYIFNKYRLEIHSNLPKCLFPLTV
**************************************:*********************
COL RTRQSGDTFKLNGRDGYKKVNRLFIDCKVPQWVRDQMPIVLDKQQRIIAVGDLYQQQTIK
N315 RTRQSGDTFKLNGRDGYKKVNRLFIDCKVPQWVRDQMPIVLDKQQRIIAVGDLYQQQTIK
NCTC8325 RTRQSGDTFKLNGRDGYKKVNRLFIDCKVPQWVRDQMPIVLDKQQRIIAVGDLYQQQTIK
Newman RTRQSGDTFKLNGRDGYKKVNRLFIDCKVPQWVRDQMPIVLDKQQRIIAVGDLYQQQTIK
USA300_FPR3757 RTRQSGDTFKLNGRDGYKKVNRLFIDCKVPQWVRDQMPIVLDKQQRIIAVGDLYQQQTIK
************************************************************
COL KWIIISKNGDE
N315 KWIIISKNGDE
NCTC8325 KWIIISKNGDE
Newman KWIIISKNGDE
USA300_FPR3757 KWIIISKNGDE
***********
- ↑ Mitsuo Kuratani, Yuka Yoshikawa, Yoshitaka Bessho, Kyoko Higashijima, Takeshi Ishii, Rie Shibata, Seizo Takahashi, Katsuhide Yutani, Shigeyuki Yokoyama
Structural basis of the initial binding of tRNA(Ile) lysidine synthetase TilS with ATP and L-lysine.
Structure: 2007, 15(12);1642-53
[PubMed:18073113] [WorldCat.org] [DOI] (P p)Tsutomu Suzuki, Kenjyo Miyauchi
Discovery and characterization of tRNAIle lysidine synthetase (TilS).
FEBS Lett: 2010, 584(2);272-7
[PubMed:19944692] [WorldCat.org] [DOI] (I p)Laura Antoine, Philippe Wolff, Eric Westhof, Pascale Romby, Stefano Marzi
Mapping post-transcriptional modifications in Staphylococcus aureus tRNAs by nanoLC/MSMS.
Biochimie: 2019, 164;60-69
[PubMed:31295507] [WorldCat.org] [DOI] (I p)