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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0553 [new locus tag: SACOL_RS02830 ]
  • pan locus tag?: SAUPAN002250000
  • symbol: SACOL0553
  • pan gene symbol?: tilS
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL0553 [new locus tag: SACOL_RS02830 ]
  • symbol: SACOL0553
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 561623..562918
  • length: 1296
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGCAGTTAAATAGTAATGGTTGGCATGTTGATGACCATATTGTTGTCGCTGTTTCTACA
    GGTATTGATAGTATGTGTTTATTGTATCAACTACTAAATGATTATAAAGATAGTTATAGA
    AAACTAACATGCTTACATGTCAATCATGGCGTTAGGTCAGCTTCAATTGAGGAAGCCAGA
    TTTTTAGAAGCATACTGCGAACGTCATCACATCGATTTACATATCAAAAAGTTAGATTTG
    TCGCATAGTCTCGACCGAAATAACAGCATTCAGAATGAAGCTCGAATTAAACGTTACGAA
    TGGTTTGATGAAATGATGAATGTATTAGAAGCGGATGTATTGCTAACGGCGCATCATTTG
    GACGATCAATTAGAAACTATTATGTATCGTATTTTTAATGGGAAATCAACACGTAATAAA
    CTAGGATTTGATGAGTTATCGAAGCGAAAAGGTTATCAGATTTATCGACCACTTTTAGCT
    GTCTCTAAAAAAGAAATAAAACAATTCCAAGAGAGATATCATATTCCATATTTTGAAGAT
    GAATCTAATAAAGATAACAAATATGTTAGAAATGATATTCGTAATAGAATTATTCCAGCT
    ATTGATGAAAATAATCAACTTAAAGTATCGCATTTATTAAAATTAAAACAATGGCATGAT
    GAACAATATGATATTTTGCAATATTCAGCTAAACAATTTATTCAAGAATTTGTGAAGTTT
    GATGAACAGTCAAAATATTTAGAGGTTTCTAGACAAGCTTTTAATAACTTACCAAACTCA
    TTAAAGATGGTTGTGTTGGACTGCCTATTATCAAAGTATTATGAGTTGTTTAATATTAGT
    GCTAAAACATACGAAGAGTGGTTTAAACAATTTAGTAGTAAGAAAGCACAATTCAGTATT
    AATCTCACGGATAAATGGATAATTCAAATCGCATATGGTAAATTAATAATAATGGCTAAA
    AATAATGGCGATACATATTTTAGAGTTCAAACAATTAAAAAGCCAGGTAATTATATTTTT
    AACAAATATCGATTAGAGATACATTCTAATTTACCAAAATGTTTATTTCCGCTTACAGTG
    AGAACACGACAAAGTGGCGATACATTTAAACTGAATGGGCGCGATGGTTATAAGAAAGTG
    AATCGCCTGTTTATAGATTGTAAAGTGCCACAGTGGGTTCGGGATCAAATGCCAATCGTA
    TTGGATAAACAACAGCGCATTATTGCGGTAGGAGATTTATATCAACAACAAACAATAAAA
    AAATGGATTATAATTAGTAAAAATGGAGATGAATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1296

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL0553 [new locus tag: SACOL_RS02830 ]
  • symbol: SACOL0553
  • description: hypothetical protein
  • length: 431
  • theoretical pI: 9.35158
  • theoretical MW: 51291.4
  • GRAVY: -0.588863

⊟Function[edit | edit source]

  • reaction:
    EC 6.3.4.19?  ExPASy
    tRNA(Ile)-lysidine synthetase (tRNA(Ile2))-cytidine34 + L-lysine + ATP = (tRNA(Ile2))-lysidine34 + AMP + diphosphate + H2O
  • TIGRFAM:
    Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA(Ile)-lysidine synthetase (TIGR02432; EC 6.3.4.-; HMM-score: 197.1)
    and 3 more
    Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA(Ile)-lysidine synthetase, C-terminal domain (TIGR02433; EC 6.-.-.-; HMM-score: 71.6)
    Unknown function General queuosine biosynthesis protein QueC (TIGR00364; HMM-score: 13.5)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis GMP synthase (glutamine-hydrolyzing), C-terminal domain (TIGR00884; EC 6.3.5.2; HMM-score: 12.7)
  • TheSEED  :
    • tRNA(Ile)-lysidine synthetase (EC 6.3.4.19)
    Miscellaneous Miscellaneous - no subcategory EC 6.3.4.- Ligases that form carbon-nitrogen bonds  tRNA(Ile)-lysidine synthetase (EC 6.3.4.19)
    and 1 more
    RNA Metabolism RNA processing and modification tRNA processing  tRNA(Ile)-lysidine synthetase (EC 6.3.4.19)
  • PFAM:
    HUP (CL0039) ATP_bind_3; PP-loop family (PF01171; HMM-score: 201.4)
    and 3 more
    PheT-TilS (CL0383) TilS_C; TilS substrate C-terminal domain (PF11734; HMM-score: 50.5)
    HUP (CL0039) QueC; Queuosine biosynthesis protein QueC (PF06508; HMM-score: 22.2)
    PAPS_reduct; Phosphoadenosine phosphosulfate reductase family (PF01507; HMM-score: 21)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002522
    • TAT(Tat/SPI): 0.00021
    • LIPO(Sec/SPII): 0.00101
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MQLNSNGWHVDDHIVVAVSTGIDSMCLLYQLLNDYKDSYRKLTCLHVNHGVRSASIEEARFLEAYCERHHIDLHIKKLDLSHSLDRNNSIQNEARIKRYEWFDEMMNVLEADVLLTAHHLDDQLETIMYRIFNGKSTRNKLGFDELSKRKGYQIYRPLLAVSKKEIKQFQERYHIPYFEDESNKDNKYVRNDIRNRIIPAIDENNQLKVSHLLKLKQWHDEQYDILQYSAKQFIQEFVKFDEQSKYLEVSRQAFNNLPNSLKMVVLDCLLSKYYELFNISAKTYEEWFKQFSSKKAQFSINLTDKWIIQIAYGKLIIMAKNNGDTYFRVQTIKKPGNYIFNKYRLEIHSNLPKCLFPLTVRTRQSGDTFKLNGRDGYKKVNRLFIDCKVPQWVRDQMPIVLDKQQRIIAVGDLYQQQTIKKWIIISKNGDE

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Cytoplasmic [1] [2]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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