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⊟Summary[edit | edit source]
- pan ID?: SAUPAN001861000
- symbol?: lplA2
- synonym:
- description?: lipoate--protein ligase A
- lipoate--protein ligase A
- lipoate-protein ligase A family protein
- lipoate--protein ligase
- lipoate-protein ligase, putative
- lipoyltransferase and lipoate-ligase family protein
- lipoyltransferase and lipoate-protein ligase
- lipoyltransferase and lipoate-protein ligase family protein
- putative lipoate-protein ligase A
descriptions from strain specific annotations:
- strand?: +
- coordinates?: 2356799..2357836
- synteny block?: BlockID0012350
- occurrence?: in 100% of 34 strains
lplA2 : free lipoic acid:protein ligase A2 [1]
S. aureus uses free lipoic acid protein ligases to salvage lipoate from the host. S. aureus makes two LplA paralogues (LplA1, LplA2) with distinct substrate specificities for their two GcvH paralogues (GcvH, GcvH-L) and three α-keto acid dehydrogenase E2 systems (PdhC, BkdB, SucB). This is further compounded by distinct in vitro/in vivo lipoylation profiles and inter-substrate lipoyltransfer by LipL. In its local genetic context, LplA2 may salvage free lipoic acid and attach it to GcvH-L which, in turn, can regulate the reactive oxygen-scavenging enoate oxidoreductase activity of OfrB.
⊟Orthologs[edit | edit source]
04-02981:
SA2981_0326
08BA02176:
C248_0381
11819-97:
MS7_0317
6850:
RSAU_000273
71193:
ST398NM01_0408
ECT-R 2:
ECTR2_287
ED133:
SAOV_0353
ED98:
SAAV_0295
HO 5096 0412:
SAEMRSA15_02830
JH1:
SaurJH1_0386
JH9:
SaurJH9_0376
JKD6008:
SAA6008_00304 (lplA1)
JKD6159:
SAA6159_00303 (lplA1)
LGA251:
SARLGA251_02890
M013:
M013TW_0308
MRSA252:
SAR0324
MSHR1132:
SAMSHR1132_03010
MSSA476:
SAS0304
Mu3:
SAHV_0324
Mu50:
SAV0327
MW2:
MW0304
RF122:
SAB0277
ST398:
SAPIG0408
T0131:
SAT0131_00332
TCH60:
HMPREF0772_10168 (lplA)
TW20:
SATW20_03950
USA300_TCH1516:
USA300HOU_0348 (lplA1)
VC40:
SAVC_01385
⊟Genome Viewer[edit | edit source]
| COL | |
| N315 | |
| NCTC8325 | |
| Newman | |
| USA300_FPR3757 | |
| JSNZ |
⊟Alignments[edit | edit source]
- alignment of orthologues: CLUSTAL format alignment by MAFFT L-INS-i (v7.307)
COL MYLIEPIRNGEYITDGAIALAMQVYVNQHIFLDEDILFPYYCDPKVEIGRFQNTAIEVNQ
N315 MYLIEPIRNGEYITDGAIALAMQVYVNQHIFLDEDILFPYYCDPKVEIGRFQNTAIEVNQ
NCTC8325 MYLIEPIRNGEYITDGAIALAMQVYVNQHIFLDEDILFPYYCDPKVEIGRFQNTAIEVNQ
Newman MYLIEPIRNGEYITDGAIALAMQVYVNQHIFLDEDILFPYYCDPKVEIGRFQNTAIEVNQ
USA300_FPR3757 MYLIEPIRNGEYITDGAIALAMQVYVNQHIFLDEDILFPYYCDPKVEIGRFQNTAIEVNQ
************************************************************
COL DYIDKHSIQVVRRDTGGGAVYVDKGAVNMCCILEQDTSIYGDFQRFYQPAIKALHTLGAT
N315 DYIDKHSIQVVRRDTGGGAVYVDKGAVNMCCILEQDTSIYGDFQRFYQPAIKALHTLGAT
NCTC8325 DYIDKHSIQVVRRDTGGGAVYVDKGAVNMCCILEQDTSIYGDFQRFYQPAIKALHTLGAT
Newman DYIDKHSIQVVRRDTGGGAVYVDKGAVNMCCILEQDTSIYGDFQRFYQPAIKALHTLGAT
USA300_FPR3757 DYIDKHSIQVVRRDTGGGAVYVDKGAVNMCCILEQDTSIYGDFQRFYQPAIKALHTLGAT
************************************************************
COL DVVQSGRNDLTLNGKKVSGAAMTLMNNRIYGGYSLLLDVNYEAMDKVLKPNRKKIASKGI
N315 DVIQSGRNDLTLNGKKVSGAAMTLMNNRIYGGYSLLLDVNYEAMDKVLKPNRKKIASKGI
NCTC8325 DVVQSGRNDLTLNGKKVSGAAMTLMNNRIYGGYSLLLDVNYEAMDKVLKPNRKKIASKGI
Newman DVVQSGRNDLTLNGKKVSGAAMTLMNNRIYGGYSLLLDVNYEAMDKVLKPNRKKIASKGI
USA300_FPR3757 DVVQSGRNDLTLNGKKVSGAAMTLMNNRIYGGYSLLLDVNYEAMDKVLKPNRKKIASKGI
**:*********************************************************
COL KSVRARVGHLREALDEKYRDITIEEFKNLMVTQILGIDDIKEAKRYELTDADWEAIDELA
N315 KSVRARVGHLREALDEKYRDITIEEFKNLMVTQILGIDDIKEAKRYELTDADWEAIDELA
NCTC8325 KSVRARVGHLREALDEKYRDITIEEFKNLMVTQILGIDDIKEAKRYELTDADWEAIDELA
Newman KSVRARVGHLREALDEKYRDITIEEFKNLMVTQILGIDDIKEAKRYELTDADWEAIDELA
USA300_FPR3757 KSVRARVGHLREALDEKYRDITIEEFKNLMVTQILGIDDIKEAKRYELTDADWEAIDELA
************************************************************
COL DKKYKNWDWNYGKSPKYEYNRSERLSSGTVDITISVEQNRIADCRIYGDFFGQGDIKDVE
N315 DKKYKNWDWNYGKSPKYEYNRSERLSSGTVDITISVEQNRIADCRIYGDFFGQGDIKDVE
NCTC8325 DKKYKNWDWNYGKSPKYEYNRSERLSSGTVDITISVEQNRIADCRIYGDFFGQGDIKDVE
Newman DKKYKNWDWNYGKSPKYEYNRSERLSSGTVDITISVEQNRIADCRIYGDFFGQGDIKDVE
USA300_FPR3757 DKKYKNWDWNYGKSPKYEYNRSERLSSGTVDITISVEQNRIADCRIYGDFFGQGDIKDVE
************************************************************
COL EALQGTKMTREDLTHQLKQLDIVYYFGNVTVEALVDMILS
N315 EALQGTKMTREDLMHQLKQLDIVYYFGNVTVESLVEMILS
NCTC8325 EALQGTKMTREDLTHQLKQLDIVYYFGNVTVEALVDMILS
Newman EALQGTKMTREDLTHQLKQLDIVYYFGNVTVEALVDMILS
USA300_FPR3757 EALQGTKMTREDLTHQLKQLDIVYYFSNVTVEALVDMILS
************* ************.*****:**:****
- ↑ Azul Zorzoli, James P Grayczyk, Francis Alonzo
Staphylococcus aureus Tissue Infection During Sepsis Is Supported by Differential Use of Bacterial or Host-Derived Lipoic Acid.
PLoS Pathog: 2016, 12(10);e1005933
[PubMed:27701474] [WorldCat.org] [DOI] (I e)Irina Laczkovich, Wei Ping Teoh, Sarah Flury, James P Grayczyk, Azul Zorzoli, Francis Alonzo
Increased flexibility in the use of exogenous lipoic acid by Staphylococcus aureus.
Mol Microbiol: 2018, 109(2);150-168
[PubMed:29660187] [WorldCat.org] [DOI] (I p)Albertina Scattolini, Konstantinos Grammatoglou, Anna Nikitjuka, Aigars Jirgensons, María Cecilia Mansilla, Björn Windshügel
Substrate Analogues Entering the Lipoic Acid Salvage Pathway via Lipoate-Protein Ligase 2 Interfere with Staphylococcus aureus Virulence.
ACS Infect Dis: 2024, 10(6);2172-2182
[PubMed:38724014] [WorldCat.org] [DOI] (I p)