PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_0320 [new locus tag: NWMN_RS01825 ]
• pan locus tag^?: SAUPAN001861000
• symbol: NWMN_0320
• pan gene symbol^?: lplA2
• synonym:
• product: lipoate-protein ligase A family protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_0320 [new locus tag: NWMN_RS01825 ]
• symbol: NWMN_0320
• product: lipoate-protein ligase A family protein
• replicon: chromosome
• strand: +
• coordinates: 369805..370827
• length: 1023
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5330159 NCBI
• RefSeq: YP_001331354 NCBI
• BioCyc:
• MicrobesOnline: 3705851 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

• 1
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  1021
  
  ATGTACTTAATAGAACCGATTAGAAATGGAGAATATATTACTGATGGTGCGATTGCACTC
  GCTATGCAAGTTTATGTTAACCAGCATATCTTTTTAGATGAAGATATTTTATTCCCTTAT
  TATTGTGATCCAAAAGTGGAAATTGGACGTTTTCAAAATACTGCTATAGAAGTGAATCAA
  GATTATATAGATAAACACAGTATTCAAGTAGTTCGCCGAGATACTGGTGGTGGCGCTGTG
  TATGTTGATAAAGGTGCCGTTAATATGTGTTGTATTTTAGAACAAGACACTTCAATTTAT
  GGTGATTTTCAACGATTTTATCAACCAGCTATAAAGGCGTTGCATACATTAGGTGCAACA
  GATGTGGTACAAAGCGGTAGAAATGATTTAACATTGAATGGTAAAAAAGTGTCAGGCGCC
  GCAATGACATTAATGAATAATCGTATTTATGGCGGTTATTCGCTATTACTTGATGTTAAT
  TATGAAGCAATGGATAAAGTGTTAAAGCCTAATCGCAAAAAGATTGCATCGAAAGGGATT
  AAATCTGTGCGCGCACGTGTTGGTCATCTTAGAGAAGCACTGGATGAAAAGTATCGTGAT
  ATAACCATTGAAGAATTTAAAAATTTAATGGTGACGCAGATTTTGGGAATCGATGACATT
  AAAGAGGCGAAACGATATGAATTAACGGATGCAGATTGGGAAGCGATTGATGAATTAGCT
  GATAAAAAGTATAAAAATTGGGATTGGAATTATGGCAAGTCACCCAAATATGAATACAAT
  CGAAGTGAAAGATTATCTTCAGGTACGGTAGACATAACAATTTCTGTTGAACAAAATCGT
  ATCGCAGATTGTCGTATTTATGGGGATTTCTTTGGACAAGGTGATATAAAAGATGTGGAA
  GAAGCATTACAAGGAACAAAAATGACAAGAGAAGATTTAACGCATCAGTTAAAGCAATTA
  GACATCGTTTATTATTTTGGCAATGTTACGGTAGAAGCATTAGTGGATATGATTTTAAGT
  TAA
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⊟Protein

⊟General

• locus tag: NWMN_0320 [new locus tag: NWMN_RS01825 ]
• symbol: NWMN_0320
• description: lipoate-protein ligase A family protein
• length: 340
• theoretical pI: 4.88005
• theoretical MW: 38846.8
• GRAVY: -0.393529

⊟Function

• reaction:
  EC 6.3.1.20?  ExPASy
  Lipoate--protein ligase ATP + (R)-lipoate + a [lipoyl-carrier protein]-L-lysine = a [lipoyl-carrier protein]-N⁶-(lipoyl)lysine + AMP + diphosphate
• TIGRFAM:
  Genetic information processing Protein fate Protein modification and repair lipoyltransferase and lipoate-protein ligase (TIGR00545; HMM-score: 243.2)
  and 1 more
  Cellular processes Cellular processes Detoxification alkylhydroperoxidase, AhpD family (TIGR00777; EC 1.11.1.15; HMM-score: 11.9)
• TheSEED  :
  • Lipoate-protein ligase A
  Amino Acids and Derivatives Alanine, serine, and glycine Glycine cleavage system  Lipoate-protein ligase A
  and 2 more
  Carbohydrates Central carbohydrate metabolism Dehydrogenase complexes  Lipoate-protein ligase A
  Cofactors, Vitamins, Prosthetic Groups, Pigments Lipoic acid Lipoic acid metabolism  Lipoate-protein ligase A
• PFAM:
  tRNA_synt_II (CL0040) LplA-B_cat; Lipoyl protein ligase A/B catalytic domain (PF21948; HMM-score: 185.7)
  and 2 more
  SufE_NifU (CL0233) Lip_prot_lig_C; Bacterial lipoate protein ligase C-terminus (PF10437; HMM-score: 97.1)
  tRNA_synt_II (CL0040) BPL_LplA_LipB; Biotin/lipoate A/B protein ligase family (PF03099; HMM-score: 34.8)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.9943
  • Cytoplasmic Membrane Score: 0.0014
  • Cell wall & surface Score: 0
  • Extracellular Score: 0.0043
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.002991
  • TAT(Tat/SPI): 0.000131
  • LIPO(Sec/SPII): 0.000507
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151220532 NCBI
• RefSeq: YP_001331354 NCBI
• UniProt: A0A0H3KE86 UniProt

⊟Protein sequence

• MYLIEPIRNGEYITDGAIALAMQVYVNQHIFLDEDILFPYYCDPKVEIGRFQNTAIEVNQDYIDKHSIQVVRRDTGGGAVYVDKGAVNMCCILEQDTSIYGDFQRFYQPAIKALHTLGATDVVQSGRNDLTLNGKKVSGAAMTLMNNRIYGGYSLLLDVNYEAMDKVLKPNRKKIASKGIKSVRARVGHLREALDEKYRDITIEEFKNLMVTQILGIDDIKEAKRYELTDADWEAIDELADKKYKNWDWNYGKSPKYEYNRSERLSSGTVDITISVEQNRIADCRIYGDFFGQGDIKDVEEALQGTKMTREDLTHQLKQLDIVYYFGNVTVEALVDMILS

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: NWMN_0316 > NWMN_0317 > NWMN_0318 > NWMN_0319 > NWMN_0320

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications