⊟Summary[edit | edit source]
- pan ID?: SAUPAN000974000
- symbol?: capA
- synonym:
- description?: capsular polysaccharide type 5 biosynthesis protein cap5A
- capsular polysaccharide type 5 biosynthesis protein cap5A
- capsular polysaccharide biosynthesis protein Cap5A
- capsular polysaccharide synthesis enzyme CapA
- capsular polysaccharide type 5/8 biosynthesis protein CapA
- capsular polysaccharide biosynthesis protein
- capsular polysaccharide synthesis protein Cap5A
- capsular polysaccharide synthesis enzyme Cap5A
- capsular polysaccharide biosynthesis protein CapA
- membrane protein CapA1
- MPA1 family polysaccharide export protein
- polysaccharide export protein, MPA1 family protein
- truncated
- truncated capsular polysaccharide synthesis enzyme Cap5A
descriptions from strain specific annotations:
- strand?: +
- coordinates?: 1230623..1231139
- synteny block?: BlockID0005080
- occurrence?: in 100% of 34 strains
capA1 : capsular polysaccharide undecaprenol phosphodiesterase and transmembrane stimulatory protein CapA1 [1]
• A crystal structure is available : 2VED
The capsule regulatory system is not intuitive and has conflicting literature. CapAB forms a bacterial tyrosine kinase (BTY) system that uses a transmembrane stimulatory protein (CapA1) to induce oligomerization of its cognate tyrosine kinase (CapB1) which as an oligomer undergoes autophosphorylation, dissociates from the complex, phosphorylates target enzymes (CapE, CapM, CapO), and activates their catalytic activity. CapA1 also exhibits phosphodiesterase activity and cleaves capsular trisaccharide phosphates from undecaprenol phosphate on the outer leaflet of the membrane. The CapAB membrane oligomerization state appears to regulate CapK flippase activity and/or CapJ polymerase activity. Unlike its paralogue CapA2, CapA1 is not inhibited by capsular polysaccharides or by N-acetylglucosamine polymers.
Historically, different capsule types were assigned their own nomenclature (i.e. Cap5A1 and Cap8A1 for the two most prevalent capsule types, CP5 and CP8). The CapA1 protein is 99% identical between Cap5A1 and Cap8A1, provides the same function, and is therefore harmonized as CapA1.
⊟Orthologs[edit | edit source]
⊟Genome Viewer[edit | edit source]
| COL | |
| N315 | |
| NCTC8325 | |
| Newman | |
| USA300_FPR3757 |
⊟Alignments[edit | edit source]
- alignment of orthologues: CLUSTAL format alignment by MAFFT L-INS-i (v7.307)
COL MESTLELTKIKEVLQKNLKILIILPLLFLIISAIVTFFVLSPKYQANTQILVNQTKGDNP
N315 MESTLELTKIKEVLQKNLKILIILPLLFLIISAIVTFFVLSPKYQANTQILVNQTKGDNP
NCTC8325 MESTLELTKIKEVLQKNLKILIILPLLFLIISAIVTFFVLSPKYQANTQILVNQTKGDNP
Newman MESTLELTKIKEVLQKNLKILIILPLLFLIISAIVTFFVLSPKYQANTQILVNQTKGDNP
USA300_FPR3757 MESTLELTKIKEVLQKNLKILIILPLLFLIISAIVTFFVLSPKYQANTQILVNQTKGDNP
************************************************************
COL QFMAQEVQSNIQLVNTYKEIVKSPRILDEVSKDLNDKYSPSKLSSMLTITNQENTQLINI
N315 QFMAQEVQSNIQLVNTYKEIVKSPRILDEVSKDLNDKYSPSKLSSMLTITNQENTQLINI
NCTC8325 QFMAQEVQSNIQLVNTYKEIVKSPRILDEVSKDLNDKYSPSKLSSMLTITNQENTQLINI
Newman QFMAQEVQSNIQLVNTYKEIVKSPRILDEVSKDLNDKYSPSKLSSMLTITNQENTQLINI
USA300_FPR3757 QFMAQEVQSNIQLVNTYKEIVKSPRILDEVSKDLNDKYSPSKLSSMLTITNQENTQLINI
************************************************************
COL QVKSGHKQDSEKIANSFAKVTSKQIPKIMSVDNVSILSKADGTAVKVAPKTVVNLIGAFF
N315 QVKSGHKQDSEKIANSFAKVTSKQIPKIMSVDNVSILSKADGTAVKVAPKTVVNLIGAFF
NCTC8325 QVKSGHKQDSEKIANSFAKVTSKQIPKIMSVDNVSILSKADGTAVKVAPKTVVNLIGAFF
Newman QVKSGHKQDSEKIANSFAKVTSKQIPKIMSVDNVSILSKADGTAVKVAPKTVVNLIGAFF
USA300_FPR3757 QVKSGHKQDSEKIANSFAKVTSKQIPKIMSVDNVSILSKADGTAVKVAPKTVVNLIGAFF
************************************************************
COL LGLVVALIYIFFKVIFDKRIKDEEDVEKELGLPVLGSIQKFN
N315 LGLVVALIYIFFKVIFDKRIKDEEDVEKELGLPVLGSIQKFN
NCTC8325 LGLVVALIYIFFKVIFDKRIKDEEDVEKELGLPVLGSIQKFN
Newman LGLVVALIYIFFKVIFDKRIKDEEDVEKELGLPVLGSIQKFN
USA300_FPR3757 LGLVVALIYIFFKVIFDKRIKDEEDVEKELGLPVLGSIQKFN
******************************************
- ↑ Vanesa Olivares-Illana, Philippe Meyer, Emmanuelle Bechet, Virginie Gueguen-Chaignon, Didier Soulat, Sylvie Lazereg-Riquier, Ivan Mijakovic, Josef Deutscher, Alain J Cozzone, Olivier Laprévote, Solange Morera, Christophe Grangeasse, Sylvie Nessler
Structural basis for the regulation mechanism of the tyrosine kinase CapB from Staphylococcus aureus.
PLoS Biol: 2008, 6(6);e143
[PubMed:18547145] [WorldCat.org] [DOI] (I p)Jakub Gruszczyk, Vanesa Olivares-Illana, Julien Nourikyan, Aurore Fleurie, Emmanuelle Béchet, Virginie Gueguen-Chaignon, Céline Freton, Magali Aumont-Nicaise, Solange Moréra, Christophe Grangeasse, Sylvie Nessler
Comparative analysis of the Tyr-kinases CapB1 and CapB2 fused to their cognate modulators CapA1 and CapA2 from Staphylococcus aureus.
PLoS One: 2013, 8(10);e75958
[PubMed:24146800] [WorldCat.org] [DOI] (I e)Marvin Rausch, Julia P Deisinger, Hannah Ulm, Anna Müller, Wenjin Li, Patrick Hardt, Xiaogang Wang, Xue Li, Marc Sylvester, Marianne Engeser, Waldemar Vollmer, Christa E Müller, Hans Georg Sahl, Jean Claire Lee, Tanja Schneider
Coordination of capsule assembly and cell wall biosynthesis in Staphylococcus aureus.
Nat Commun: 2019, 10(1);1404
[PubMed:30926919] [WorldCat.org] [DOI] (I e)Satsawat Visansirikul, Stephen A Kolodziej, Alexei V Demchenko
Staphylococcus aureus capsular polysaccharides: a structural and synthetic perspective.
Org Biomol Chem: 2020, 18(5);783-798
[PubMed:31922180] [WorldCat.org] [DOI] (I p)