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⊟Summary[edit | edit source]
- pan ID?: SAUPAN000993000
- symbol?: capO
- synonym:
- description?: UDP-N-acetyl-D-mannosamine dehydrogenase
- UDP-N-acetyl-D-mannosamine dehydrogenase
- capsular polysaccharide biosynthesis protein Cap5O
- cap5O protein/UDP-N-acetyl-D-mannosaminuronic acid dehydrogenase
- capsular polysaccharide biosynthesis protein CapO
- capsular polysaccharide synthesis protein Cap8O
- capsular polysaccharide synthesis UDP-N-acetyl-D-mannosaminuronic acid dehydrogenase CapO, putative
- nucleotide sugar dehydrogenase
- type 8 capsular polysaccharide synthesis protein Cap8O
- capsular polysaccharide synthesis enzyme Cap8O
- capsular polysaccharide synthesis enzyme CapO
- NDP-N-acetyl-D-galactosaminuronic acid dehydrogenase
- UDP-glucose/GDP-mannose dehydrogenase
- UDP-N-acetyl-D-mannosamine 6-dehydrogenase
descriptions from strain specific annotations:
- strand?: +
- coordinates?: 1261910..1263172
- synteny block?: BlockID0005260
- occurrence?: in 100% of 34 strains
capO : capsule polysaccharide precursor synthesis enzyme UDP-N-acetylmannosamine 6-dehydrogenase [1]
• A crystal structure is available : 3OJL
Staphylococci can produce up to 15 different capsules based on variants of the core trisaccharide N-acetyl-D-mannosaminouronate (ManNAcA) - N-acetyl-L-fucosamine (L-FucNAc) - N-acetyl-D-fucosamine (D-FucNAc). CapO catalyzes the second of two steps in the biosynthesis of UDP-ManNAcA, oxidizing the C-6 hydroxyl group of UDP-N-acetyl-D-mannosamine.
Historically, different capsule types were assigned their own nomenclature (i.e. Cap5O and Cap8O for the two most prevalent capsule types, CP5 and CP8). The CapO protein is 98% identical between Cap5O and Cap8O, provides the same function, and is therefore harmonized as CapO.
⊟Orthologs[edit | edit source]
04-02981:
SA2981_0164 (capO)
08BA02176:
C248_0152 (capO)
11819-97:
MS7_0157 (wecC)
6850:
RSAU_000117 (capO)
71193:
ST398NM01_0177
ECT-R 2:
ECTR2_120
ED133:
SAOV_0110
ED98:
SAAV_0131 (cap5O)
HO 5096 0412:
SAEMRSA15_01290 (capO)
JH1:
SaurJH1_0154
JH9:
SaurJH9_0149
JKD6008:
SAA6008_00141 (cap5O)
JKD6159:
SAA6159_00147 (cap5O)
JSNZ:
JSNZ_000106 (cap8O)
LGA251:
SARLGA251_01370 (capO)
M013:
M013TW_0153
MRSA252:
SAR0165 (capO)
MSHR1132:
SAMSHR1132_01370
MSSA476:
SAS0138
Mu3:
SAHV_0162 (capO)
Mu50:
SAV0163 (capO)
MW2:
MW0138 (cap8O)
RF122:
SAB0104 (capO)
ST398:
SAPIG0177
T0131:
SAT0131_00154 (zur)
TCH60:
HMPREF0772_10335 (epsD)
TW20:
SATW20_01740 (capO)
USA300_TCH1516:
USA300HOU_0177 (cap5O)
VC40:
SAVC_00585
⊟Genome Viewer[edit | edit source]
| COL | |
| N315 | |
| NCTC8325 | |
| Newman | |
| USA300_FPR3757 |
⊟Alignments[edit | edit source]
- alignment of orthologues: CLUSTAL format alignment by MAFFT L-INS-i (v7.307)
COL MKLTVVGLGYIGLPTSIMFAKHGVDVLGVDINQQTIDKLQSGQISIEEPGLQEVYEEVLS
N315 MKLTVVGLGYIGLPTSIMFAKHGVDVLGVDINQQTIDKLQSGQISIEEPGLQEVYEEVLS
NCTC8325 MKLTVVGLGYIGLPTSIMFAKHGVDVLGVDINQQTIDKLQSGQISIEEPGLQEVYEEVLS
Newman MKLTVVGLGYIGLPTSIMFAKHGVDVLGVDINQQTIDKLQSGQISIEEPGLQEVYEEVLS
USA300_FPR3757 MKLTVVGLGYIGLPTSIMFAKHGVDVLGVDINQQTIDKLQSGQISIEEPGLQEVYEEVLS
************************************************************
COL SGKLKVSTTPDASDVFIIAVPTPNNDDQYRSCDISLVMRALDSILSFLEKGNTIIVESTI
N315 SGKLKVSTTPEASDVFIIAVPTPNNDDQYRSCDISLVMRALDSILPFLEKGNTIIVESTI
NCTC8325 SGKLKVSTTPDASDVFIIAVPTPNNDDQYRSCDISLVMRALDSILSFLEKGNTIIVESTI
Newman SGKLKVSTTPDASDVFIIAVPTPNNDDQYRSCDISLVMRALDSILSFLEKGNTIIVESTI
USA300_FPR3757 SGKLKVSTTPDASDVFIIAVPTPNNDDQYRSCDISLVMRALDSILSFLEKGNTIIVESTI
**********:**********************************.**************
COL APKTMDDFVKPVIENLGFTIGEDIYLVHCPERVLPGKILEELVHNNRIIGGVTEACIEAG
N315 APKTMDDFVKPVIENLGFTIGEDICLVHCPERVLPGKILEELVHNNRIIGGVTKACIEAG
NCTC8325 APKTMDDFVKPVIENLGFTIGEDIYLVHCPERVLPGKILEELVHNNRIIGGVTEACIEAG
Newman APKTMDDFVKPVIENLGFTIGEDIYLVHCPERVLPGKILEELVHNNRIIGGVTEACIEAG
USA300_FPR3757 APKTMDDFVKPVIENLGFTIGEDIYLVHCPERVLPGKILEELVHNNRIIGGVTEACIEAG
************************ ****************************:******
COL KRVYRTFVQGEMIETDARTAEMSKLMENTYRDVNIALANELTKICNNLNINVLDVIEMAN
N315 KYVYRTFVQGEMIETDARTAEMSKLMENTYRDVNIALANELTKICNNLNINVLDVIEMAN
NCTC8325 KRVYRTFVQGEMIETDARTAEMSKLMENTYRDVNIALANELTKICNNLNINVLDVIEMAN
Newman KRVYRTFVQGEMIETDARTAEMSKLMENTYRDVNIALANELTKICNNLNINVLDVIEMAN
USA300_FPR3757 KRVYRTFVQGEMIETDARTAEMSKLMENTYRDVNIALANELTKICNNLNINVLDVIEMAN
* **********************************************************
COL KHPRVNIHQPGPGVGGHCLAVDPYFIIAKDPENAKLIQTGREINNSMPAYVVDTTKQIIK
N315 KHPRVNIHQPGPGVGGHCLAVDPYFIIAKDPENAKLIQTGREINNSMPAYVVDTTKQIIK
NCTC8325 KHPRVNIHQPGPGVGGHCLAVDPYFIIAKDPENAKLIQTGREINNSMPAYVVDTTKQIIK
Newman KHPRVNIHQPGPGVGGHCLAVDPYFIIAKDPENAKLIQTGREINNSMPAYVVDTTKQIIK
USA300_FPR3757 KHPRVNIHQPGPGVGGHCLAVDPYFIIAKDPENAKLIQTGREINNSMPAYVVDTTKQIIK
************************************************************
COL VLSGNKVTVFGLTYKGDVDDIRESPAFDIYELLNQEPDIEVCAYDPHVELDFVEHDMSHA
N315 ALSGNKVTVFGLTYKGDVDDIRESPAFDIYELLNQEPDIEVCAYDPHVELDFVEHDMSHA
NCTC8325 VLSGNKVTVFGLTYKGDVDDIRESPAFDIYELLNQEPDIEVCAYDPHVELDFVEHDMSHA
Newman VLSGNKVTVFGLTYKGDVDDIRESPAFDIYELLNQEPDIEVCAYDPHVELDFVEHDMSHA
USA300_FPR3757 VLSGNKVTVFGLTYKGDVDDIRESPAFDIYELLNQEPDIEVCAYDPHVELDFVEHDMSHA
.***********************************************************
COL VKDASLVLILSDHSEFKNLSDSHFDKMKHKVIFDTKNVVKSSFEDVSYYNYGNIFNFIDK
N315 VKDASLVLILSDHSEFKNLSDSHFDKMKHKVIFDTKNVVKSSFEDVSYYNYGNIFNFIDK
NCTC8325 VKDASLVLILSDHSEFKNLSDSHFDKMKHKVIFDTKNVVKSSFEDVSYYNYGNIFNFIDK
Newman VKDASLVLILSDHSEFKNLSDSHFDKMKHKVIFDTKNVVKSSFEDVSYYNYGNIFNFIDK
USA300_FPR3757 VKDASLVLILSDHSEFKNLSDSHFDKMKHKVIFDTKNVVKSSFEDVSYYNYGNIFNFIDK
************************************************************
- ↑ Satsawat Visansirikul, Stephen A Kolodziej, Alexei V Demchenko
Staphylococcus aureus capsular polysaccharides: a structural and synthetic perspective.
Org Biomol Chem: 2020, 18(5);783-798
[PubMed:31922180] [WorldCat.org] [DOI] (I p)