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NCBI: 26-AUG-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus N315
- locus tag: SA0144 [new locus tag: SA_RS00890 ]
- pan locus tag?: SAUPAN000974000
- symbol: capA
- pan gene symbol?: capA
- synonym:
- product: capsular polysaccharide synthesis protein Cap5A
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SA0144 [new locus tag: SA_RS00890 ]
- symbol: capA
- product: capsular polysaccharide synthesis protein Cap5A
- replicon: chromosome
- strand: +
- coordinates: 167409..168077
- length: 669
- essential: no DEG other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 1122919 NCBI
- RefSeq: NP_373386 NCBI
- BioCyc: see SA_RS00890
- MicrobesOnline: 102412 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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661ATGGAAAGTACATTAGAATTAACAAAAATTAAAGAAGTATTACAAAAAAACTTGAAGATT
TTAATTATTTTACCGCTATTATTTTTAATTATTAGCGCTATTGTTACATTTTTCGTCTTA
TCACCTAAATATCAAGCTAATACTCAAATCTTAGTGAATCAAACTAAGGGTGACAATCCT
CAGTTTATGGCACAAGAGGTTCAAAGTAATATTCAACTTGTAAATACGTATAAAGAAATT
GTTAAAAGTCCTAGAATTTTAGATGAGGTGTCAAAGGACTTAAATGATAAGTATTCACCA
TCTAAATTGTCGAGTATGTTGACAATTACAAACCAAGAAAATACGCAACTTATCAACATC
CAAGTTAAAAGTGGTCATAAACAAGATTCGGAAAAAATTGCGAATAGCTTCGCTAAAGTT
ACAAGTAAACAAATTCCGAAGATTATGAGTGTGGATAACGTATCAATTTTATCTAAAGCA
GACGGTACAGCAGTTAAAGTCGCACCAAAAACTGTAGTGAATCTAATCGGTGCATTCTTT
TTAGGATTAGTTGTCGCGCTTATATATATCTTCTTCAAAGTAATTTTCGATAAGCGAATT
AAAGATGAAGAAGATGTAGAGAAAGAATTAGGATTGCCTGTATTGGGTTCAATTCAAAAA
TTTAATTAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SA0144 [new locus tag: SA_RS00890 ]
- symbol: CapA
- description: capsular polysaccharide synthesis protein Cap5A
- length: 222
- theoretical pI: 9.86158
- theoretical MW: 24854
- GRAVY: 0.0869369
⊟Function[edit | edit source]
- TIGRFAM: Transport and binding proteins Carbohydrates, organic alcohols, and acids polysaccharide export protein, MPA1 family (TIGR01006; HMM-score: 322.3)and 4 morechain length determinant protein EpsF (TIGR03017; HMM-score: 42)Transport and binding proteins Carbohydrates, organic alcohols, and acids exopolysaccharide transport protein family (TIGR01005; HMM-score: 30.8)Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides polysaccharide chain length determinant protein, PEP-CTERM locus subfamily (TIGR03007; HMM-score: 16.2)HAD hydrolase, TIGR01459 family (TIGR01459; HMM-score: 12.9)
- TheSEED :
- Capsular polysaccharide synthesis enzyme CapA
- Tyrosine-protein kinase transmembrane modulator EpsC
Cell Wall and Capsule Capsular and extracellular polysacchrides Exopolysaccharide Biosynthesis Tyrosine-protein kinase transmembrane modulator EpsCand 1 more - PFAM: no clan defined Wzz; Chain length determinant protein (PF02706; HMM-score: 82.7)and 7 moreGNVR; G-rich domain on putative tyrosine kinase (PF13807; HMM-score: 24.4)LapA_dom; Lipopolysaccharide assembly protein A domain (PF06305; HMM-score: 13.3)DUF1189; Protein of unknown function (DUF1189) (PF06691; HMM-score: 12.5)DUF2663; Protein of unknown function (DUF2663) (PF10864; HMM-score: 11.9)DUF5469; Family of unknown function (DUF5469) (PF17561; HMM-score: 10.4)DUF3671; Protein of unknown function (PF12420; HMM-score: 6.8)DUF3985; Protein of unknown function (DUF3985) (PF13153; HMM-score: 6.5)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic Membrane
- Cytoplasmic Score: 0.32
- Cytoplasmic Membrane Score: 9.55
- Cellwall Score: 0.12
- Extracellular Score: 0.01
- Internal Helices: 2
- LocateP: Multi-transmembrane
- Prediction by SwissProt Classification: Membrane
- Pathway Prediction: Sec-(SPI)
- Intracellular possibility: 0.17
- Signal peptide possibility: -1
- N-terminally Anchored Score: 1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.054736
- TAT(Tat/SPI): 0.000669
- LIPO(Sec/SPII): 0.003427
- predicted transmembrane helices (TMHMM): 2
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MESTLELTKIKEVLQKNLKILIILPLLFLIISAIVTFFVLSPKYQANTQILVNQTKGDNPQFMAQEVQSNIQLVNTYKEIVKSPRILDEVSKDLNDKYSPSKLSSMLTITNQENTQLINIQVKSGHKQDSEKIANSFAKVTSKQIPKIMSVDNVSILSKADGTAVKVAPKTVVNLIGAFFLGLVVALIYIFFKVIFDKRIKDEEDVEKELGLPVLGSIQKFN
⊟Experimental data[edit | edit source]
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator: CodY (repression) regulon
CodY (TF) important in Amino acid metabolism; RegPrecise
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ Daniela Keinhörster, Shilpa Elizabeth George, Christopher Weidenmaier, Christiane Wolz
Function and regulation of Staphylococcus aureus wall teichoic acids and capsular polysaccharides.
Int J Med Microbiol: 2019, 309(6);151333
[PubMed:31362856] [WorldCat.org] [DOI] (I p) - ↑ S Sau, J Sun, C Y Lee
Molecular characterization and transcriptional analysis of type 8 capsule genes in Staphylococcus aureus.
J Bacteriol: 1997, 179(5);1614-21
[PubMed:9045821] [WorldCat.org] [DOI] (P p)